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Engineering CELLS,
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Research Highlights

Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paperbased matrix

Published in “Shu Hui Neo, Ka Yan Chung, Jia Min Quek and Heng-Phon Too, Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paperbased matrix, Scientific Reports 2017 7(1): 16686.”

Shotgun Glycomics Identifies Tumor-Associated Glycan Ligands Bound by an Ovarian Carcinoma-Specific Monoclonal Antibody

Published in “B. Liau, B. Tan, G. Teo, P. Zhang, A. Choo & P. M. Rudd, Shotgun Glycomics Identifies Tumor-Associated Glycan Ligands Bound by an Ovarian Carcinoma-Specific Monoclonal Antibody, Scientific Reports 2017 7(1): 14489.”

Evaluation and use of disaccharides as energy source in protein-free mammalian cell cultures

Published in “Dawn Sow Zong Leong, Janice Gek Ling Tan, Christine Lin Chin, Shi Ya Mak, Ying Swan Ho, Say Kong Ng, Evaluation and use of disaccharides as energy source in protein-free mammalian cell cultures, Scientific Reports 2017 7: 45216.”

Lipidomic Profiling of Lung Pleural Effusion Identifies Unique Metabotype for EGFR-Mutants in Non-Small Cell Lung Cancer

Published in “Ying Swan Ho, Lian Yee Yip, Nurhidayah Basri, Vivian Su Hui Chong, Chin Chye Teo, Eddy Tan, Kah Ling Lim, Gek San Tan, Xulei Yang, Si Yong Yeo, Mariko Si Yue Koh, Anantham Devenand, Angela Takano, Daniel Shao Weng Tan, Tony Kiat Hon Lim, Lipidomic Profiling of Lung Pleural Effusion Identifies Unique Metabotype for EGFR-Mutants in Non-Small Cell Lung Cancer, Scientific Reports 2017 6: 35110.”

Exploiting the Protein Corona from Cell Lysate on DNA Functionalized Gold Nanoparticles for Enhanced mRNA Translation

Published in “Kian Ping Chan, Yang Gao, Jeremy Xianwei Goh, Dewi Susanti, Eugenia Li Ling Yeo, Sheng-Hao Chao, James Chen Yong Kah, Exploiting the Protein Corona on DNA Functionalized Gold Nanoparticles for Enhanced mRNA Translation, ACS Applied Materials & Interfaces 2017 9(12): 10408-10417.”

Tyrosine kinase c-Abl regulates the survival of plasma cells

Published in “Yan-Feng Li, Shengli Xu, Yuhan Huang, Xijun Ou & Kong-Peng Lam, Tyrosine kinase c-Abl regulates the survival of plasma cells, Scientific Reports 2017 7: 40133.”

Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis

Published in “Jiyun Zheng, Tan Heng Liang, Paul Matsudaira, Andre Choo, Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis, Cell Death and Differentiation 2017 24(3):546-558.”

Superior red blood cell generation from human pluripotent stem cells via a novel microcarrier based embryoid body platform

Published in “Jaichandran Sivalingam, Alan Tin-Lun Lam, Hong Yu Chen, Bin Xia Yang, Allen Kuan-Liang Chen, Shaul Reuveny, Yuin-Han Loh and Steve Kah-Weng Oh, Superior red blood cell generation from human pluripotent stem cells via a novel microcarrier based embryoid body platform, Tissue Engineering Part C: Methods 2016 22(8): 765-780.”

Small colony variants and single nucleotide variations in Pf1 region of PB1 phage-resistant Pseudomonas aeruginosa

Published in “Wee Siang Lim, Kevin Kai Siang Phang, Andy Hee-Meng Tan, Sam Fong-Yau Li and Dave Siak-Wei Ow, Small colony variants and single nucleotide variations in Pf1 region of PB1 phage-resistant Pseudomonas aeruginosa, Frontiers in Microbiology 2016 7: 282.”

Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures

Published in “Hock Chuan Yeo, Sherwin Ting, Romulo Martin Brena, Geoffrey Koh, Allen Chen, Siew Qi Toh, Yu Ming Lim, Steve Kah Weng Oh & Dong-Yup Lee, Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Culturess, Scientific Reports 2016 6: 31068.”

Expansion in microcarrier-spinner cultures improves the chondrogenic potential of human early mesenchymal stem cells

Published in “Youshan Melissa Lin, Jessica Fang Yan Lim, Jialing Lee, Mahesh Choolani, Jerry Kok Yen Chan, Shaul Reuveny, Steve Kah Weng Oh, Expansion in microcarrier-spinner cultures improves the chondrogenic potential of human early mesenchymal stem cells, Cytotherapy 2016 18: 740-753.”

Loss of miR-182 affects B-cell extrafollicular antibody response

Published in “Yan-Feng Li, Xijun Ou, Shengli Xu, Zi-Bing Jin, Naoharu Iwai, and Kong-Peng Lam, Loss of miR-182 affects B-cell extrafollicular antibody response, Immunology 2016 148(2): 140-149.”

Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy

Published in “Tessa Rui Min Tan, Kong Meng Hoi, Peiqing Zhang, Say Kong Ng, Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy, PLoS One 2016 11(3): e0152112.”

Use of a novel cytotoxic HEXIM1 peptide in the directed breast cancer therapy

Published in “Shu Hui Neo, Qiao Jing Lew, Ser Mei Koh, Lu Zheng, Xuezhi Bi, and Sheng-Hao Chao, Use of a novel cytotoxic HEXIM1 peptide in the directed breast cancer therapy, Oncotargets 2015 7(5): 5483-5494.”

Loss of Fas Apoptosis Inhibitory Molecule Leads to Spontaneous Obesity and Hepatosteatosis

Published in “Jianxin Huo, Yi Ma, Jian-Jun Liu, Ying Swan Ho, Sylvia Liu, Li Ying Soh, Shuwen Chen, Shengli Xu, Weiping Han, An Hong, Su Chi Lim, Kong-Peng Lam, Loss of Fas Apoptosis Inhibitory Molecule Leads to Spontaneous Obesity and Hepatosteatosis, Cell Death and Disease 2016 7: e2091”

A carbohydrate-binding affinity ligand for the specific enrichment of glycoproteins

Published in “Chen Chen, Graziella El Khoury, Peiqing Zhang, Pauline M. Rudd, Christopher R. Lowe, A carbohydrate-binding affinity ligand for the specific enrichment of glycoproteins, Journal of Chromatography A 2016 1444: 8-20”

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

Published in “Kah Fai Chan, Wahyu Shahreel, Corrine Wan, Gavin Teo, Noor Hayati, Shi Jie Tay, Wen Han Tong, Yuansheng Yang, Pauline M. Rudd, Peiqing Zhang, and Zhiwei Song, Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies, Biotechnology Journal 2015 11(3): 399-414”

Non-immunospecific association of immunoglobulin G with chromatin during elution from protein A inflates host contamination, aggregate content, and antibody loss

Published in “Pete Gagnon, Rui Nian, Yuansheng Yang, Qiaohui Yang, Chiew Ling Lim, Non-immunospecific association of immunoglobulin G with chromatin during elution from protein A inflates host contamination, aggregate content, and antibody loss, Journal of Chromatography A 2015 1408: 151-160”

A genetic algorithm-based approach for pre-processing metabolomics and lipidomics LC-MS data

Published in “Hock Chuan Yeo, Bevan Kai-Sheng Chung, William Chong, Ju Xin Chin, Kok Siong Ang, Meiyappan Lakshmanan, Ying Swan Ho, Dong-Yup Lee, A genetic algorithm-based approach for pre-processing metabolomics and lipidomics LC-MS data, Metabolomics 2015 12: 5”

Mir-17~92 regulates bone marrow homing of plasma cells and production of immunoglobulin G 2c

Published in “Shengli Xu, Xijun Ou, Jianxin Huo, Kristen Lim, Yuhan Huang, Sheena Chee & Kong-Peng Lam, Mir-17~92 regulates bone marrow homing of plasma cells and production of immunoglobulin G 2c, Nature Communications 2015 6: 6764”

Heat shock protein 27 overexpression in CHO cells delays activation of caspases to improve recombinant monoclonal antibody titer

Published in “Janice G.L. Tan, Yih Yean Lee, Tianhua Wang, Miranda G. S. Yap, Tin Wee Tan, Say Kong Ng, Heat shock protein 27 overexpression in CHO cells delays activation of caspases to improve recombinant monoclonal antibody titer, Biotechnology Journal 2015 10(5): 790-800”

Serum-free media formulations are cell line–specific and require optimization for microcarrier culture

Published in “Kah Yong Tan, Kim Leng Teo, Jessica Fang Yan Lim, Allen Kuan-Liang Chen, Shaul Reuveny, Steve Kah Weng Oh, Serum-free media formulations are cell line–specific and require optimization for microcarrier culture, Cytotherapy 2015 17(8): 1152–1165”

Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography

Published in “Pete Gagnon, Rui Nian, Denise Leong and Aina Hoi, Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography, Journal of Chromatography A 2015 1395: 136–142”

Bruton's tyrosine kinase phosphorylates DDX41 and activates its binding of dsDNA and STING to initiate type 1 interferon response

Published in “Koon-Guan Lee, Susana Soo-Yeon Kim, Lin Kui, Dominic Chih-Cheng Voon, Marjorie Mauduit, Pradeep Bist, Xuezhi Bi, Natasha Ann Pereira, Chengcheng Liu, Bindu Sukumaran, Laurent Rénia, Yoshiaki Ito and Kong-Peng Lam, Cell Reports 10(7):1055-65”

Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells

Published in “Jake Chng, Tianhua Wang, Rui Nian, Ally Lau, Kong Meng Hoi, Steven C.L. Ho, Peter Gagnon, Xuezhi Bi, Yuansheng Yang, Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells, mAbs 2015 7(2): 403-412”

mAb C19 targets a novel surface marker for the isolation of human cardiac progenitor cells from human heart tissue and differentiated hESCs

Published in “Hau Wan Leung, Asja T. Moerkamp, Jayanthi Padmanabhan, Sze-Wai Ng, Marie-José Goumans, Andre Choo, mAb C19 targets a novel surface marker for the isolation of human cardiac progenitor cells from human heart tissue and differentiated hESCs, Journal of Molecular and Cellular Cardiology 2015 82: 228-237”

Cardiomyocyte differentiation of pluripotent stem cells with SB203580 analogues correlates with Wnt pathway CK1 inhibition independent of p38 MAPK signaling

Published in “Filip Laco, Joo-Leng Low, Jasmin Seow, Tsung Liang Woo, Qixing Zhong, Jayasree Seayad, Shaul Reuveny, David A. Elliott, Christina L. L. Chai and Steve Oh, Journal of Molecular and Cellular Cardiology 2014 80: 56-70”

Adaptor protein DOK3 promotes plasma cell differentiation by regulating the expression of programmed cell death 1 ligands

Published in “Xijun Ou, Shengli Xu, Yan-Feng Li, and Kong-Peng Lam, Adaptor protein DOK3 promotes plasma cell differentiation by regulating the expression of programmed cell death 1 ligands, Proceedings of the National Academy of Sciences (PNAS) 2014, 111(31): 11431-11436”

Generation of novel monoclonal antibodies for the enrichment and characterization of human corneal endothelial cells (hCENC) necessary for the treatment of corneal endothelial blindness

Published in “Vanessa Ding, Angela Chin, Gary Peh, Jodhbir S Mehta and Andre Choo, Generation of novel monoclonal antibodies for the enrichment and characterization of human corneal endothelial cells (hCENC) necessary for the treatment of corneal endothelial blindness, mAbs 2014 6(6): 1439-1452”.

Chromatin-Mediated Depression of Fractionation Performance on Electronegative Multimodal Chromatography Media, its Prevention, and Ramifications for Purification of Immunoglobulin G

Published in “Pete Gagnon, Rui Nian, Lihan Tan, Jason Cheong, Veronica Yeo, Yuansheng Yang, and Hui Theng Gan, Journal of Chromatography A 2014 1374: 145–155”

Aberrant presentation of self-lipids by autoimmune B cells depletes peripheral iNKT cells

Published in “Andy Hee-Meng Tan, William Pooi-Kat Chong, Sze-Wai Ng, Nurhidayah Basri, Shengli Xu, and Kong-Peng Lam, Aberrant presentation of self-lipids by autoimmune B cells depletes peripheral iNKT cells, Cell Reports 2014 9(1): 24-31”.

GCN5 inhibits XBP-1S-mediated transcription by antagonizing PCAF action

Published in “Qiao Jing Lew, Kai Ling Chu, Yi Ling Chia, Benjamin Soo, Jia Pei Ho, Chew Har Ng, Hui Si Kwok, Cheng-Ming Chiang, Yao Chang, and Sheng-Hao Chao, GCN5 inhibits XBP-1S-mediated transcription by antagonizing PCAF action, Oncotarget 2015 6(1): 271-287”.

Time-resolved Video Analysis and Management System for Monitoring Cardiomyocyte Differentiation Processes and Toxicology Assays

Published in “Sherwin Ting, Seaw Jia Liew, Francis Japson, Fuchun Shang, Wee Keat Chong, Shaul Reuveny, Jo Yew Tham, Xiang Li and Steve Oh, Time-resolved Video Analysis and Management System for Monitoring Cardiomyocyte Differentiation Processes and Toxicology Assays, Biotechnology Journal 2014 9(5): 675-683”

DOK3 is required for IFN-β production by enabling TRAF3/TBK1 complex formation and IRF3 activation

Published in “Susana Soo-Yeon Kim, Koon-Guan Lee, Ching-Siang Chin, Say-Kong Ng, Natasha Ann Pereira, Shengli Xu, and Kong-Peng Lam, DOK3 is required for IFN-β production by enabling TRAF3/TBK1 complex formation and IRF3 activation, Journal of Immunology 2014; 193(2): 840-848”

Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture

Published in “Alan Tin-Lun Lam, Allen Kuan-Liang Chen, Jian Li, William R. Birch, Shaul Reuveny and Steve Kah-Weng Oh, Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture, Stem Cell Research & Therapy 5(5): 110”

Shp1 signaling is required to establish the long-lived bone marrow plasma cell pool

Published in “Yan-Feng Li, Shengli Xu, Xijun Ou & Kong-Peng Lam, Shp1 signaling is required to establish the long-lived bone marrow plasma cell pool, Nature Communications 2014; 5: 4273”.

Nonspecific Interactions of Chromatin with Immunoglobulin G and Protein A, and Their Impact on Purification Performance

Published in “Pete Gagnon, Rui Nian, Jeremy Lee, Lihan Tan, Sarah Maria Abdul Latiff, Chiew Ling Lim, Cindy Chuah, Xuezhi Bi, Yuansheng Yang, Wei Zhang and Hui Theng Gan, Nonspecific Interactions of Chromatin with Immunoglobulin G and Protein A, and Their Impact on Purification Performance, Journal of Chromatography A 2014; 1340: 68-78”.

Codon Optimization OnLine (COOL): a web-based multi-objective optimization platform for synthetic gene design

Published in “Ju Xin Chin, Bevan Kai-Sheng Chung and Dong-Yup Lee, Codon Optimization OnLine (COOL): a web-based multi-objective optimization platform for synthetic gene design, Bioinformatics 2014; 30(15): 2210-2212”.

Microbubble-mediated sonoporation for highly efficient transfection of recalcitrant human B- cell lines

Published in “Charlene Li Ling Yong, Dave Siak-Wei Ow, Tandiono Tandiono, Lisa Li Mei Heng, Ken Kwok-Keung Chan, Claus-Dieter Ohl, Evert Klaseboer, Siew-Wan Ohl, and Andre Boon-Hwa Choo, Microbubble-mediated sonoporation for highly efficient transfection of recalcitrant human B- cell lines, Biotechnology Journal 2014; 9(8):1081-1087”.

Golgi phosphoprotein 3 mediates the Golgi localization and function of protein O-linked mannose β-1,2-Nacetlyglucosaminyltransferase 1

Published in “Natasha A. Pereira, Helen X. Pu, Hazel Goh and Zhiwei Song, Golgi phosphoprotein 3 mediates the Golgi localization and function of protein O-linked mannose β-1,2-Nacetlyglucosaminyltransferase 1, Journal of Biological Chemistry 2014; 289(21): 14762-14770”.

Considerations in designing systems for large scale production of human cardiomyocytes from pluripotent stem cells

Published in “Allen Chen, Sherwin Ting, Jasmin Seow, Shaul Reuveny and Steve Oh, Considerations in designing systems for large scale production of human cardiomyocytes from pluripotent stem cells, Stem Cell Research & Therapy 2014; 5(12): 1-13”.

An Internal Ribosome Entry Site (IRES) Mutant Library for Tuning Expression Level of Multiple Genes in Mammalian Cells

Published in “Esther Y. C. Koh, Steven C. L. Ho, Mariati, Zhiwei Song, Xuezhi Bi, Muriel Bardor, Yuansheng Yang, An Internal Ribosome Entry Site (IRES) Mutant Library for Tuning Expression Level of Multiple Genes in Mammalian Cells, PLOS One 2014; 8(12): e82100”

Multiple Reaction Monitoring Mass Spectrometry for the Discovery and Quantification of O-Glcnac Modified Proteins

Published in “Julien Jean Pierre Maury, Daniel Ng, Xuezhi Bi, Muriel Bardor and Andre Boon-Hwa Choo, Multiple Reaction Monitoring Mass Spectrometry for the Discovery and Quantification of O-Glcnac Modified Proteins, Analytical Chemistry 2013; 86(1):395-402”.

Inhibition of human cytomegalovirus replication by overexpression of CREB1

Published in “Yi Ling Chia, Chew Har Ng, Philip Lashmit, Kai Ling Chu, Qiao Jing Lew, Jia Pei Ho, Hsueh Lee Lim, Peter Morin Nissom, Mark F. Stinski, and Sheng-Hao Chao, Inhibition of human cytomegalovirus replication by overexpression of CREB1, Antiviral Research 2014; 102: 11–22”.

Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function

Published in “John S.Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang, Yuanxing Zhang, Zhiwei Song, Biotechnology Journal 2013; 9(1):100-109”.

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