IMB-IMCB Joint Electron Microscopy suite provides a broad range of solutions for qualitative and quantitative 2D and 3D ultrastructural analysis of various types of samples from multiprotein complexes to cells, tissues and bio-composites.

  • Direct ultrastructural analysis of purified macromolecular structures (protein complexes, membrane vesicles, microbes, nanoparticles etc.) using negative staining and TEM
  • Direct ultrastructural cryo-TEM analysis of purified macromolecular structures processed by cryo-fixation (vitrification) using plunge freezing
  • Direct immuno-electron microscopy (DIEM) detecting antigen(s) on purified macromolecular structures using gold-conjugated antibodies, negative staining and TEM
  • TEM analysis of ultra-thin sections from chemically fixed and resin embedded samples of adherent cells (vertical or horizontal sections), suspension cells, tissues, biopsies and other type of samples
  • Cryo-fixation (vitrification) by high-pressure freezing followed by (i) cryo-sectionning and cryo-TEM (CEMOVIS), or (ii) by freeze substitution, low-temperature resin embedding, sectioning and conventional TEM imaging
  • TEM analysis of 3D surface replica from vitrified cells, organelles, membrane layers and emulsions after freeze-fracturing, etching and metal-shadowing
  • Fine-structure immuno-cytochemistry using gold-conjugated antibodies for antigen detection and antigen colocalization on thawed cryo-sections (Tokuyasu method)  or on sections from resin-embedded samples
  • Electron tomography and 3D reconstruction of partial cell volumes from serial sections of resin-embedded samples (Tilt series acquisition, alignment, back-projection and 3D surface rendering)
  • Scanning electron microscopy (SEM) for surface morphology analysis of various type of samples processed by critical-point drying (or HMDS evaporation) and sputter coating
  • Correlative light and electron microscopy (CLEM) for correlation of dynamic features and phenotypes obtained by (time-lapse) fluorescence imaging with ultrastructural resolution of TEM
  • Stereology and quantitative and qualitative analysis of imaging datasets