• Dermatotoxicology • Models for Organ Specific, Developmental & Genetic Toxicity • PBTK & Exposure Modelling • Allergen Prediction • Toxicity Mode-of-Action Discovery (ToxMAD) Platform
This project deploys co culture of donor-matched, human iPSC-derived liver-resident macrophages - Kupffer cells (iKCs) and iPSC-derived hepatocytes (iHep) for development of inflammation-sensitive in vitro liver model for testing hepatotoxicity of a wide range of compounds drugs, food and consumer care products, cosmetics and environmental toxins.
Prof. Hanry Yu
Dr. Florent GinhouxDr. Farah TASNIM
This project aims to combine our method for generating induced pluripotent stem cell (iPSC)-derived human renal cells with our award-winning high-content screening (HCS) platform for predicting nephrotoxicity in humans with high accuracy. The resulting iPSC-based HCS platform for nephrotoxicity prediction will be validated with a robust number of compounds, and the predictive performance metrics will be determined. In-depth characterization of the modes-of-action of selected compounds will be performed in collaboration with other IFCS groups.
Dr. Daniele ZinkDr. Lit-Hsin Loo
Dr. Sreetama BasuDr. Riga Tawo
Although significant understanding about the mechanisms of nanotoxicity has been made over the last decade, limited attention has been given to making nanotoxicology testing fit for regulatory purposes. Collaborating with the Singapore Food Agency (SFA), we aim to develop reliable and robust methods for genotoxicity testing using commercially available 3D-Reconstituted Human Intestinal Epithelia and in-house co-culture models, and to validate these for regulatory use.
Dr. Benjamin SmithAssoc. Prof. David Ian LeavesleyDr. Hui Kheng Lim
Dr. Zheng Ming WangDr. Jasmine LiDr. Calvin YeoDr. Man Ling Chau
Assoc. Prof. Kee Woei NgDr. Mustafa Hussain Kathawal
μP-hPST can recapitulate morphological disruption of spatiotemporally controlled mesoendoderm development upon treatment with known teratogens. The model has been validated using 34 pharmaceutical compounds and classified compounds with 97% accuracy, 100% specificity and 93% sensitivity and successfully identified misclassified drugs. This project focuses on testing the teratogenicity of environmental, industrial and agro chemicals using this model with the bigger aim of improving the overall predictivity of developmental toxicity models.
Prof. Hanry YuDr. Samuel WongDr. Farah Tasnim