Since established in 2011, the Animal Gene Editing Laboratory (AGEL) has successfully generated more than 60 genetically-modified rodent models for research or as service. These include gene specific knockout/knockin models by traditional ES targeting and indel mutation & single nucleotide mutagenesis models by more recently developed CRISPR/Cas9 & TALEN methods.
Our comprehensive services include strategy design, synthesis and preparation of molecular reagents, targeting vector construction, microinjection, screening and detailed genotyping analysis of mutant founders, and production of germline transmission F1 heterozygotes carrying desired mutations.
Key Services
1) Gene knockout by CRISPR or TALEN
- Indel reading frame-shift mutation
- Exon or full gene deletion (up to 20 kb)