Engineering CELLS,
   Engineering RESPONSES

Transcriptomic Analysis

The profiling of gene expression at the RNA level is a useful tool to study the state of cells in various biological systems, such as cell differentiation and cell stress responses. This snapshot or fingerprinting of cell state is particularly useful as the first study in target identification for cell engineering or process modification.

The Microarray Group has developed expertise in studying genetic circuitry underlying cellular metabolism using microarray and next-generation sequencing (NGS) technologies. We are experienced in sample preparation, transcriptomic analysis* and bioinformatics analysis of samples from cell cultures and primary tissues. Some of the services that we provide include:

  • RNA and DNA extraction
  • cDNA synthesis and library preparation
  • Microarray chip processing*
  • Gene expression analysis using commercial microarray gene chips
  • Validation of differential gene expression by quantitative RT-PCR
  • Whole genome, RNA and ChIP sequencing on Illumina sequencing platform*
  • NGS analysis including differential gene expression analysis, cluster analysis, pathway analysis and visualization, gene set enrichment analysis, de novo / reference-guided genome or transcriptome assembly
  • Metagenomics of bacterial and fungal communities (16S, 18S, ITS amplicon sequencing*)

* Microarray chip processing and Illumina sequencing are performed in partnership with our internal and external associates

 

For enquires regarding Transcriptomic Analysis, please email: tan_tuang_kin@bti.a-star.edu.sg