About Us Banner
Engineering CELLS,
   Engineering RESPONSES

Analytics

Biopharmaceutical drugs are mainly recombinant glycoproteins produced in cell culture. These macromolecules are inherently heterogeneous structurally, most notably in terms of glycosylation. Certain structural attributes have detrimental effect on drug safety profile and therapeutic performance, and therefore, should be routinely monitored for safety and quality assurance of therapeutic proteins.

The assessment of product safety and quality through product and process analysis is the major task of the Analytics Group. The Group has anchored technologies in monitoring vital components involved in cell culture, especially amino acids, to assist medium design and optimization of the operating strategy in running bioprocesses.

Extensive biopharmaceutical product characterization is also routinely carried out at Analytics for protein aggregation, conformation and glycosylation analysis utilizing a suite of technologies consisting of MALDI-TOF mass spectrometry (MS), High Performance Liquid Chromatography (HPLC), Capillary Electrophoresis (CE), and Multi-Angle Light Scattering device (MALS) and other established biochemical assays.

In particular, a comprehensive glycan analytical platform has been built up based on the MALDI-TOF and HPLC systems for both N- and O-glycan profiling of recombinant glycoprotein drugs (IgG, EPO, IFN-γ, etc.). Additionally, the glyco-analytics platform has been applied to the study of glycosylation status of host cell lines and tissues. This enabling analytical platform now allows us to monitor many glycosylation-associated critical quality attributes (gCQAs) such as site occupancy, sialic acid types and their levels, as well as relative abundance of glycan species, to ensure the safety and efficacy of biopharmaceutical products.

The group is also developing new methods and platforms for rapid, more detailed glycosylation characterization at higher throughput to strengthen the glycomics and glycoproteomics capabilities for the development of novel drugs and biosimilars in Singapore.

expression engineering1 pic

People

Dr. Terry Nguyen-Khuong

PhD in Proteomics/Peptidomics (2011), University of New South Wales, Australia

Research Focus/Interests
  • High throughput and comprehensive biochemical analytics such as proteomics, glycoproteomics and glycomics from complex Biosystems
  • Glyco-related bioinformatics
  • Recombinant biopharmaceuticals
  • Biomarker discovery

Dr. Ian Walsh

PhD in Bioinformatics (2009), University College Dublin, Ireland

Research Focus/Interests
  • Novel adaptive computational models to annotate and interpret raw biological data
  • Bioinformatics software and methods development
  • Machine learning
  • Data analytics

Dr. Matthew Choo

PhD in Life Sciences (2016), Imperial College London, UK

Research Focus/Interests
  • Characterising glycan epitopes targeted by novel monoclonal antibody therapies
  • Glycomic analysis of human disease and disease-causing organisms using MALDI tandem mass spectrometry
  • Functionalising insights gained from disease glycomes into novel therapies
  • Developing advanced and comprehensive glycopeptide mass spectrometry with the Orbitrap Fusion
  • Characterising and site-mapping the post-translational modifications of disease-associated glycoproteins
  • Fostering cross-disciplinary collaborations to tackle medical and biological questions

Dr. Katherine Wongtrakul-Kish

PhD in Stem Cell Glycobiology (2016), Macquarie University, Sydney, Australia

Research Focus/Interests
  • Systems Glycobiology
  • Detailed glycan structural characterisation by LC-MS/MS
  • Glycomic and glycoproteomic changes in disease states for biomarker discovery
  • Glyco-informatics towards high-throughput glycan and glycopeptide characterisation

Dr. Sophie Zhao

PhD in Computational Biology (2014), National University of Singapore, Singapore

Research Focus/Interests
  • Docking and molecular modeling of molecules
  • Computational analysis of Omics data
  • Bioinformatics tool development

Year 2018

    Dawn Sow Zong Leong, Brian Kah Hui Teo, Janice Gek Ling Tan, Hayati Kamari, Yuan Sheng Yang, Peiqing Zhang and Say Kong Ng (2018) Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody. Scientific Reports 8(1): 4037

Year 2017

    Liau B, Tan B, Teo G, Zhang P, Choo A, Rudd P.M (2017) Shotgun Glycomics Identifies Tumor-Associated Glycan Ligands Bound by an Ovarian Carcinoma-Specific Monoclonal Antibody. Scientific Reports 7(1): 14489

    Jessna H.M. Yeo, Steven C.L. Ho, Susanto Woen, Mariati, Esther Koh, Tay Shi Jie, Peiqing Zhang, Yuansheng Yang (2017) Optimized selection marker and CHO host cell combinations for generating high monoclonal producing cell lines. Biotechnology Journal (Published Online)

    Jian'er Lin, Shu Hui Neo, Steven C. L. Ho, Jessna H. M. Yeo, Tianhua Wang, Wei Zhang, Xuezhi Bi, Sheng-Hao Chao and Yuansheng Yang (2017) Impact of Signal Peptides on Furin-2A Mediated Monoclonal Antibody Secretion in CHO Cells. Biotechnology Journal 12: 9

    Sally S. L. Yap, Terry Nguyen-Khuong, Pauline M. Rudd and Sylvie Alonso (2017) Dengue Virus Glycosylation: What Do We Know?. Frontiers in Microbiology 8: 1415

    Damiano Piovesan, Ian Walsh, Giovanni Minervini and Silvio C. E. Tosatto (2017) FELLS: fast estimator of latent local structure. Bioinformatics 33(12): 1889-1891

    Faraaz Noor Khan Yusufi, Meiyappan Lakshmanan, Ying Swan Ho, Bernard Liat Wen Loo, Pramila Ariyaratne, Yuansheng Yang, Say Kong Ng, Tessa Rui Min Tan, Hock Chuan Yeo, Hsueh Lee Lim, Sze Wai Ng, Ai Ping Hiu, Chung Ping Chow, Corrine Wan, Chen Shuwen, Gavin Teo, Gao Song, Ju Xin Chin, Xiaoan Ruan, Ken Wing Kin Sung, Wei-Shou Hu, Miranda Gek Sim Yap, Muriel Bardor, Niranjan Nagarajan and Dong-Yup Lee (2017) Mammalian systems biotechnology reveals global cellular adaptations in a recombinant CHO cell line. Cell Systems 4(5): 530-542.e6

    Matthew S. F. Choo, Vanessa Ding, Heng Liang Tan, Brian Liau, Lauren Hartley-Tassell, Roberto Castangia, Omar Belgacem, Stuart M. Haslam, Anne Dell and Andre B. H. Choo (2017) Characterization of H Type 1 and Type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4. Journal of Biological Chemistry 292(15): 6163-6176

Year 2016

    Ian Walsh, Sophie Zhao, Matthew Campbell, Christopher H Taron, Pauline M Rudd (2016) Quantitative profiling of glycans and glycopeptides: an informatics' perspective. Current Opinion in Structural Biology 40: 70-80

    Steven C.L. Ho, Rui Nian, Susanto Woen, Jake Chng, Peiqing Zhang and Yuansheng Yang (2016) Impact of hydrolysates on monoclonal antibody productivity, purification and quality in Chinese hamster ovary cells. Journal of Bioscience and Bioengineering 122(4): 499–506

    Beate Hintersteiner, Nico Lingg, Peiqing Zhang, Susanto Woen, Kong Meng Hoi, Stefan Stranner, Susanne Wiederkum, Oliver Mutschlechner, Manfred Schuster, Hans Loibner and Alois Jungbauer (2016) Charge heterogeneity: basic antibody charge variants with increased binding to Fc receptors. mAbs 8(8): 1548-1560

    Chen Chen, Graziella El Khoury, Peiqing Zhang, Pauline M. Rudd, Christopher R. Lowe (2016) A carbohydrate-binding affinity ligand for the specific enrichment of glycoproteins. Journal of Chromatography A 1444: 8-20

    Tessa Rui Min Tan, Kong Meng Hoi, Peiqing Zhang, Say Kong Ng (2016) Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy. PLoS One 11(3): e0152112

    Peiqing Zhang, Susanto Woen, Tianhua Wang, Brian Liau, Sophie Zhao, Chen Chen, Yuansheng Yang, Zhiwei Song, Mark R. Wormald, Chuanfei Yu and Pauline M. Rudd (2016) Challenges of glycosylation analysis and control: an integrated approach to producing optimal and consistent therapeutic drugs. Drug Discovery Today 21(5): 740-765

Year 2015

    Kah Fai Chan, Wahyu Shahreel, Corrine Wan, Gavin Teo, Noor Hayati, Shi Jie Tay, Wen Han Tong, Yuansheng Yang, Pauline M. Rudd, Peiqing Zhang and Zhiwei Song (2015) Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies. Biotechnology Journal 11(3): 399-414

    Steven C. L. Ho, Tianhua Wang, Zhiwei Song, Yuansheng Yang (2015) IgG aggregation mechanism for CHO cell lines expressing excess heavy chains and strategies to reduce the aggregates. Molecular Biotechnology 57(7): 625-634

    Janice G.L. Tan, Yih Yean Lee, Tianhua Wang, Miranda G. S. Yap, Tin Wee Tan and Say Kong Ng (2015) Heat shock protein 27 overexpression in CHO cells delays activation of caspases to improve recombinant monoclonal antibody titer. Biotechnology Journal 10(5): 790-800

    Chin Chye Teo, William Pooi Kat Chong, Eddy Tan, Nurhidayah Binte Basri, ZhenJie Low, Ying Swan Ho (2015) Advances in sample preparation and analytical techniques for lipidomics study of clinical samples. Trends in Analytical Chemistry 66: 1-18

    Jake Chng, Tianhua Wang, Rui Nian, Ally Lau, Kong Meng Hoi, Steven C.L. Ho, Peter Gagnon, Xuezhi Bi, Yuansheng Yang (2015) Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells. mAbs 7(2): 403-412

Year 2014

    John SY Goh, Yingwei Liu, Kah Fai Chan, Corinne Wan, Gavin Teo, Peiqing Zhang, Yuanxing Zhang and Zhiwei Song (2014) Producing recombinant therapeutic glycoproteins with enhanced sialylation using CHO-gmt4 glycosylation mutant cells. Bioengineered 5(4): 269–273

    Wan Ping Loh, Bernard Loo, Lihan Zhou, Peiqing Zhang, Dong-Yup Lee, Yuansheng Yang and Kong Peng Lam (2014) Overexpression of microRNAs enhances recombinant protein production in Chinese hamster ovary cells. Biotechnology Journal (Published Online)

Year 2013

    Peiqing Zhang, Tianhua Wang, Muriel Bardor, Zhiwei Song (2013) Deciphering the O-glycomics for the development and production of biopharmaceuticals. Pharmaceutical Bioprocessing 1(1): 89–104

    Nico Lingg, Eddy Tan, Beate Hintersteiner, Muriel Bardor and Alois Jungbauer (2013) Highly linear pH gradients for analyzing monoclonal antibody charge heterogeneity in the alkaline range. Journal of Chromatography A (Published Online)

    John S.Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang, Yuanxing Zhang and Zhiwei Song (2013) Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnology Journal (Published Online)

    Elodie Mathieu-Rivet, Martin Scholz, Carolina Arias, Flavien Dardelle, Stefan Schulze, François Le Mauff, Gavin Teo, Ana Karina Hochma, Amaya Blanco-Rivero, Corinne Loutelier-Bourhis, Marie-Christine Kiefer-Meyer, Christian Fufezan, Carole Burel, Patrice Lerouge, Flor Martinez, Muriel Bardor and Michael Hippler (2013) Exploring the N-Glycosylation Pathway in Chlamydomonas Reinhardtii Unravels Novel Complex Structures. Molecular and Cellular Proteomics (Published Online)

    Riccardo Torosantucci, Victor S. Sharov, Miranda van Beers, Vera Brinks, Christian Scho¨neich and Wim Jiskoot (2013) Identification of Oxidation Sites and Covalent Cross-Links in Metal Catalyzed Oxidized Interferon Beta-1a: Potential Implications for Protein Aggregation and Immunogenicity. Molecular Pharmaceutics 10: 2311-2322

    Steven C.L. Ho, Esther Y.C. Koh, Miranda van Beers, Monika Mueller, Corrine Wan, Gavin Teo, Zhiwei Song, Yen Wah Tongb, Muriel Bardor, Yuansheng Yang (2013) Control of IgG LC:HC ratio in stably transfected CHO cells and study of the impact on expression, aggregation, glycosylation and conformational stability. Journal of Biotechnology 165: 157-166

Year 2012

    Nico Lingg, Peiqing Zhang, Zhiwei Song and Muriel Bardor (2012) The sweet tooth of biopharmaceuticals: Importance of recombinant protein glycosylation analysis. Biotechnology Journal 7: 1 - 11

    Peiqing Zhang, Kah Fai Chan, Ryan Haryadi, Muriel Bardor and Zhiwei Song (2012) CHO glycosylation mutants as potential host cells to produce therapeutic proteins with enhanced efficacy. Advances in Biochemical Engineering/Biotechnology Volume: Future Trends in Biotechnology. Springer (Published Online)

    Peiqing Zhang, Ryan Haryadi, Kah Fai Chan, Gavin Teo, John Goh, Natasha Ann Pereira, Huatao Feng, and Zhiwei Song (2012) Identification of functional elements of the GDP-Fucose transporter SLC35C1 using a novel CHO mutant. Glycobiology 22 (7): 897-911

Page 1 of 2   |    Total 54 Record(s)
Start Prev 12 Next End