SINGLE CELL GENOMICS

FACULTY-IN-CHARGE

The GIS Single-Cell Omics Centre (GIS-SCOC) is a laboratory located within the Institute dedicated to accelerating the understanding of how individual cells work, and how diagnosis and treatment might be enhanced through insight derived from single cells. GIS-SCOC allows researchers within and outside A-STAR to collaborate and accelerate the development of breakthrough scientific and medical advancements, applications and therapeutics.

The mission of the SCOC is based on the concept that the cell is the basic building block of life. The human body is composed of billions of individual cells representing thousands of cell-types all intricately communicating with one another. One of the goals of this centre is to define biological systems at this level, whether in diseased states or normal development, by analysing the transcriptomes and genomes of individual cells. By profiling the transcriptome of many individual cells within a population, one gains unparalleled insight into the diversity of cell types and how they communicate between themselves. Such a level of understanding provides mechanistic insights into how cells self-renew, differentiate, evolve (both between species and within cancers), and respond to infection and/or drug treatments. The centre allows access state of the art single-cell genomics so that scientists and clinicians within the Singapore research community remain on the cutting edge of scientific research.

The centre features technologies from 3 major players in the single cell field: 10x Genomics, Fluidigm and Wafergen-Takara. 10X genomics facilitates high-throughput capture of 1K - 10K single cells, and also across multiple samples; the C1 Single-Cell Auto Prep Systems enables capture and imaging of upto 800 cells in one microfluidic chip and high-throuput quantification of nuclei acids through BioMark HD Systems; while the Wafergen platform serves as a mid-range platform to capture, image upto ~1800 cells. GIS-SCOC has experienced personnel to consult, design, and execute the best strategies for your experimental needs, ranging from single cell capture, DNA/ RNA sequencing to single-cell data analysis.

The GIS Single Cell Omics Centre stands as a prime example of a mutually beneficial academic industry partnership model that fully exploits the capabilities of the institute in technology and experimental and computational biology.

Solutions at Single-Cell Omics Centre

Single - Cell Genomics Solution Workflow Applications

10x Chromium controller

10x genomics system
generates thousands of
single cell droplets
per minute for
down stream application

  1. Single cell droplet
    generation in
    10x chromium controller
  2. RT in thermocycler
  3. cDNA Amplification
    and cleanup
  4. Library preparation
    and size selection cleanup
  5. Sequencing
Single cell gene expression
Single cell immune profiling
Genome sequencing
Exome sequencing
Single cell CNV
De novo Assembly

Coming Soon …
Single Cell ATACseq

C1™ Single-Cell
Auto Prep System

Automated single-cell
isolation and
preparation system for
genomic analysis

C1™ Script Builder™
Build custom single-cell
application; invent
new methods to explore
cellular heterogeneity

  1. CAPTURE CELLS
  2. VERIFY
  3. LYSE
  4. EXTRACT AND AMPLIFY
    NUCLEIC ACIDS
  5. HARVEST
Single-Cell Targeted Gene Expression
Single-Cell miRNA Expression Profiling
Single-Cell Whole Transcriptome Analysis
Single-Cell Targeted DNA Sequencing
Single-Cell Whole Exome Sequencing
Single-Cell Whole Genome Sequencing

Biomark HD™ System

Multipurpose real-time
PCR system that performs
genotyping, gene expression
profiling, quantitative real-time
digital PCR (qdPCR),
and single-cell analysis

  1. PRIME
  2. TRANSFER
  3. LOAD
  4. RUN
  5. ANALYSE
Gene Expression
Single-Cell Gene Expression
SNP Genotyping
Protein Expression
Copy Number Variation
Sample Identification

Access Array™ System

Builds amplicon libraries
for all major NGS platforms: 
Illumina® GAllx™,
HiSeq®, MiSeq™
Sequencers; Ion Torrent™
PGM™ and Roche® 454

  1. LOAD
  2. AMPLIFY
  3. HARVEST
Targeted Resequencing
Target Enrichment
Sample Barcoding for
Multiplex Sequencing
Sequencing Library Prep Using
Amplicon Tagging

ICELL8™ Single-Cell System

Icell8 system rapidly
dispense thousands of
single cell and analyze
cells of any size with
unbiased cell isolation

  1. Dispense cells, controls and fiducials
  2. Image wells and select cells for processing
  3. Dispense RT to the selected cells
  4. cDNA preparation, extraction and purification
Single-Cell mRNA sequencing



Single - Cell Genomics Analysis software

Fluidigm® Singular™ Analysis Toolset
An open source solution for visualizing and
interpreting single-cell data, the Singular
Analysis Toolset offers graphics to identify
gene expression and mutation patterns in
populations of cells, zoom in on the most
critical targets and eliminate outliers.

Biomark Real-Time PCR Analysis
Establish standard curves, calculate delta delta Ct;
Automatically determine ideal threshold setting

10x Genomics Analysis Pipeline Software
Cell Ranger, Cell Ranger DNA,
Long Ranger
Supernova

10x Genomics Visualization Software
Loupe Cell Browser
Loupe V(D)J browser
Loupe scDNA Browser
Loupe Genome Browser




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