Bioengineering Systems

A Scalable and Sensitive Steatosis Chip with Long-term Perfusion of in situ Differentiated HepaRG Organoids.

Teng, Y., Zhao, Z., Tasnim, F., Huang, X., & Yu, H. (2021).
Biomaterials, 120904.

Design and Fabrication of the Vertical-Flow Bioreactor for Compaction Hepatocyte Culture in Drug Testing Application

Zhu, L., Wang, Z., Xia, H., & Yu, H. (2021).
Biosensors, 11(5), 160.

Effects of Hydrophobicity on Antimicrobial Activity, Selectivity, and Functional Mechanism of Guanidinium-Functionalized Polymers

Tan, J., Zhao, Y., Hedrick, J. L., & Yang, Y. Y. (2021).
Advanced Healthcare Materials, 2100482.

Digital CRISPR-based Method for the Rapid Detection and Absolute Quantification of Nucleic Acids

Wu, X., Tay, J.K., Goh, C.K., Chan, C., Lee, Y.H., Springs, S.L., Loh, K.S., Lu, T.K. and Yu, H., 2021.
Biomaterials, 274, 120876.

Recent Advances in Models of Immune-Mediated Drug-Induced Liver Injury

Tasnim, F., Huang, X., Lee, C. Z. W., Yu, H., & Ginhoux, F. (2021).
Frontiers in Toxicology, 3, 22.

Fight Bacteria with Bacteria: Bacterial Membrane Vesicles as Vaccines and Delivery Nanocarriers against Bacterial Infections

Gan, Y., Li, C., Peng, X., Wu, S., Li, Y., Tan, J.P., Yang, Y.Y., Yuan, P. and Ding, X. (2021). 
Nanomedicine: Nanotechnology, Biology and Medicine, 35, 102398.

Hyaluronic Acid-Green Tea Catechin Conjugates as a Potential Therapeutic Agent for Rheumatoid Arthritis

Lee, F., Bae, K. H., Ng, S., Yamashita, A., & Kurisawa, M. (2021).  
RSC Advances, 11(24), 14285-14294.

Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Yap, T.L., Hong, S.Y., Soh, J.H., Ravichandraprabhu, L., Lim, V.W., Chan, H.M., Ong, T.Z., Chua, Y.P., Koh, S.E., Wang, H. and Leo, Y.S. (2021). Emerging infectious diseases, 27(5), 1427.

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Ng, I.C., Zhang, L., Shen, N.N.Y.Y., Soong, Y.T., Ng, C.W., Koh, P.K.S., Zhou, Y. and Yu, H. (2021).
Journal of Visualized Experiments: Jove, (170).

pH-Degradable Polymers as Impermanent Antimicrobial Agents for Environmental Sustainability

D. S. W. Lim, Y. Yuan and Y. Zhang
ACS Applied Bio Materials, 4 (2021), 1544–1551; DOI: 10.1021/acsabm.0c01402

Accelerated Antimicrobial Discovery via Deep Generative Models and Molecular Dynamics Simulations

P. Das, T. Sercu, K. Wadhawan, I. Padhi, S. Gehrmann, F. Cipcigan, V. Chenthamarakshan, H. Strobelt, C. dos Santos, P.-Y. Chen, Y. Y. Yang, J. P. K. Tan, J. Hedrick, J. Crain and A. Mojsilovic
Nature Biomedical Engineering (2021); DOI: 10.1038/s41551-021-00689-x

Novel Live Cell Fluorescent Probe for Human-Induced Pluripotent Stem Cells Highlights Early Reprogramming Population

S. Sriram, N.-Y. Kang, S. Subramanian, T. Nandi, S. Sudhagar, Q. Xing, G. J.-L. Tong, A. K.-L. Chen, T. C. Srijaya, P. Tan, Y.-H. Loh, Y.-T. Chang and S. Sugii
Stem Cell Research & Therapy, 12 (2021), 113;DOI: 10.1186/s13287-021-02171-6

CD10 marks non-canonical PPARγ-independent adipocyte maturation and browning potential of adipose-derived stem cells

Smarajit Chakraborty, Wee Kiat Ong, Winifred W. Y. Yau, Zhihong Zhou, K. N. Bhanu Prakash, Sue-Anne Toh, Weiping Han, Paul M. Yen and Shigeki Sugii.
Stem cell Research and Therapy, 12:109.

Can an in vitro Hair Drug Model be Developed Using Dermal Papilla Cells Alone?

T. C. Lim, M. F. Leong, H. Lu, C. Du and A. C. A. Wan
Experimental Dermatology (2021); DOI: 10.1111/exd.14297

CD10 Marks Non-Canonical PPARγ-Independent Adipocyte Maturation and Browning Potential of Adipose-Derived Stem Cells

S. Chakraborty, W. K. Ong, W. W. Y. Yau, Z. Zhou, K. N. B. Prakash, S.-A. Toh, W. Han, P. M. Yen and S. Sugii
Stem Cell Research & Therapy, 12 (2021), 109;DOI: 10.1186/s13287-021-02179-y

Food Security and Nutrition- A Systematic Approach

A. C. A. Wan, B. C. U. Tai and C. Du
Trends in Food Science & Technology (2021); DOI: 10.1016/j.tifs.2020.12.024

Carboxylic acid-functionalized polycarbonates as bone cement additives for enhanced and sustained release of antibiotics

ZC Liang, C Yang, X Ding, JL Hedrick, W Wang, YY Yang
Journal of Controlled Release

Synthetic peptide hydrogels as 3D scaffolds for tissue engineering

X Ding, H Zhao, Y Li, AL Lee, Z Li, M Fu, C Li, YY Yang, P Yuan
Advanced Drug Delivery Reviews

Clinical utility of Epstein‐Barr virus DNA and other liquid biopsy markers in nasopharyngeal carcinoma

Tan R, Phua SKA, Soong YL, Oon LLE, Chan KS, Lucky SS, Mong J, Tan MH, Lim CM
Cancer Commun (Lond), 2020 Sep 28. doi: 10.1002/cac2.12100

Genetic Alphabet Expansion Technology by Creating Unnatural Base Pairs

M. Kimoto, I. Hirao
Chem. Soc. Rev., (2020). in press.

Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA

M. Kimoto, S.H.G. Soh, and I. Hirao
Chembiochem., (2020) doi: 10.1002/cbic.202000057

Combination of guanidinium and quaternary ammonium polymers with distinctive antimicrobial mechanisms achieving a synergistic antimicrobial effect

J. Leong, C. Yang, B. Q. Tan, S. Hor, J. L. Hedrick, Y. Y. Yang*
Biomaterials Science 2020, DOI: 10.1039/D0BM00752H

Branched α-helical peptides enhanced antitumor efficacy and selectivity

X. Wang, Y. Zheng, G. Zhong, C. Bao, S. Liu, N. Wiradharma,  W. Fan, Y. Y. Yang*, X. Wang, Y. Huang
Biomaterials Science 2020 (IF: 6.183)

Bile canaliculi contract autonomously by releasing calcium into hepatocytes via mechanosensitive calcium channel

K. Gupta, I. C. Ng, G. M. Balachander, B. P. Nguyen, L. Tucker-Kellogg, B. C. Low and H. Yu
259 (2020) 120283

Biomimetic niches reveal the minimal cues to trigger apical lumen formation in single hepatocytes

Y. Zhang, R. De Mets, C. Monzel, V. Acharya, P. Toh, J. F. L. Chin, N. Van Hul, I. C. Ng, H. Yu, S. S. Ng, S. T. Rashid and V. Viasnoff
Nature Materials, 19 (2020) 1026-1035

Decellularized liver as a translucent ex vivo model for vascular embolization evaluation

Y. N. Gao, Z. H. Li, Y. Hong, T. T. Li, X. Y. Hu, L. Y. Sun, Z. C. Chen, Z. J. Chen, Z. H. Luo, X. Wang, J. Kong, G. L. Li, H. L. Wang, H. L. Leo, H. Yu, L. Xi and Q. Y. Guo
Biomaterials, 240 (2020) 119855

A vascular-liver chip for sensitive detection of nutraceutical metabolites from human pluripotent stem cell derivatives

F. Yu, Y. T. Goh, H. Li, B. C. Narmada, M. Ni, G. L. Xu, T. –M. Hsieh, Y. –C. Toh, C. Cheung, C. Iliescu and H. Yu
Biomaterials, 240 (2020) 119855

Functional Stem Cell Sorting via Integrative Droplet Synchronization

G. Sun, Y. Teng, Z. Zhao, L. F. Cheow, H. Yu and C. –H. Chen
Analytical Chemistry, 92 (2020) 7915-7923

Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing

F. Tasnim, N. H. Singh, E. K. F. Tan, J. Xing, H. Li, S. Hissette, S. Manesh, J. Fulwood, K. Gupta, C. W. Ng, S. Xu, J. Hill and H. Yu
Scientific Reports, 10 (2020) 4768

IIL-13-driven alterations in hepatic cholesterol handling contributes to hypercholesterolemia in a rat model of minimal change disease

L. D. Low, L. Lu, C. Y. Chan, J. Chen, H. H. Yang, H. Yu, C. G. L. Lee, K. H. Ng and H. K. Yap
Clinical science, 134 (2020) 225-237

Oxidative Stress Mediates Depot-specific Functional Differences of Human Adipose-derived Stem Cells

Sriram, S.; Yuan, C.; Chakraborty, S.; Tay, W.; Park, M.; Shabbir, A.; Toh, S.A.; Han, W.; and Sugii, S.
Chapter 26, Quantitative Magnetic Resonance Imaging. Elsevier.

Physical and Physiological Properties of Fat.

Sugii, S.; and Velan, S.S.
Chapter 26, Quantitative Magnetic Resonance Imaging. Elsevier.

Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing

Xing, Q.R.; El Farran, C.A.; Gautam, P.; Chuah, Y.S.; Warrier, T.; Toh, C.X.D.; Kang, N.Y.; Sugii, S., Chang, Y.T.; Xu, J.; Collins, J.J.; Daley, G.Q.; Li, H.; Zhang, L.F.; and Loh, Y.H.
Science Adv.

Metal Catalyst-Free Oxidative C-C Bond Cleavage of a Lignin Model Compound by H2O2 in Formic acid

X Li, YG Zhang
ChemSusChem, 2020(13)1740-1745

Antibiotic resistance mitigation: the development of alternative general strategies

SM Riduan, A Armugan, YG Zhang
J. Mater. Chem. B, 2020(8)6317-6321

Highly efficient imidazolium-containing oligomers for preventing MRSA biofilm and postoperative spinal infection

JB Li, YJ Wang, MX Che, X Ji, J Liu, Y Sun, CS Xiao, YG Zhang, SK Zhang
Eur. Polymer J., 2020(137)109910

Rational Design of Gram-Specific Antimicrobial Imidazolium Tetramers To Combat MRSA

Y Yuan, W Shi, R Li, D S W Lim, A Armugam, YG Zhang
ACS Biomater. Sci. Eng, 2020

Injectable gelatin hydroxyphenyl propionic acid hydrogel protects human retinal progenitor cells (hRPCs) from shear stress applied during small-bore needle injection

Pierre Colombe Dromel, Deepti Singh, Alfredo Alexander-Katz, Motoichi Kurisawa, Myron Spector, Michael Young
Applied Materials Today, 19, 100602 (2020)

A Macromolecule Reversing Antibiotic Resistance Phenotype and Repurposing Drugs as Potent Antibiotics

Ding X, Yang C, Moreira W, Yuan P, Periaswamy B, de Sessions PF, Zhao H, Tan J, Lee A, Ong KX, Park N, Liang ZC, Hedrick JL, Yang YY
Advanced Science 7 (2020) 2001374 (IF: 15.840)

Functional Stem Cell Sorting via Integrative Droplet Synchronization

Sun G, Teng Y, Zhao Z, Cheow LF, Yu H, and Chen C
Anal. Chem. 2020;. [PMID: 32378402]

Biomimetic niches reveal the minimal cues to trigger apical lumen formation in single hepatocytes

Zhang Y, De Mets R, Monzel C, Acharya V, Toh P, Chin JFL, Van Hul N, Ng IC, Yu H, Ng SS, Tamir Rashid S, and Viasnoff V
Nat Mater 2020;. [PMID: 32341512]

Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing

Tasnim F, Singh NH, Tan EKF, Xing J, Li H, Hissette S, Manesh S, Fulwood J, Gupta K, Ng CW, Xu S, Hill J, and Yu H
Sci Rep 2020; 10(1):4768. [PMID: 32179810]

Decellularized liver as a translucent ex vivo model for vascular embolization evaluation

Gao Y, Li Z, Hong Y, Li T, Hu X, Sun L, Chen Z, Chen Z, Luo Z, Wang X, Kong J, Li G, Wang H, Leo HL, Yu H, Xi L, and Guo Q
Biomaterials 2020; 240:119855. [PMID: 32070824]

IL-13-driven alterations in hepatic cholesterol handling contributes to hypercholesterolemia in a rat model of minimal change disease

Low LD, Lu L, Chan C, Chen J, Yang HH, Yu H, Lee CGL, Ng K, and Yap H
Clin. Sci. 2020; 134(2):225-237. [PMID: 31934720]

A vascular-liver chip for sensitive detection of nutraceutical metabolites from human pluripotent stem cell derivatives

Fang Yu, Yeek Teck Goh, Huan Li, Narmada Balakrishnan Chakrapani, Ming Ni, Guo Lin Xu, Tseng-Ming Hsieh, Yi-Chin Toh, Christine Cheung, Ciprian Iliescu, and Hanry Yu
Biomicrofluidics 14, 034108 (2020)

Cost-effective robust synthesis of methacrylic cellulosic sponge for organoid culture

Z Liu, F Tasnim, S Ong, S Shen, XZ Huang, ELS Fong, H Yu
Cellulose 27 (1), 171-184 (2020)

Cell membrane-engineered hybrid soft nanocomposites for biomedical applications

Y. Li, Y. Gan, C. Li, Y. Y. Yang, P. Yuan, X. Ding
J. Mater. Chem. B, 2020 DOI:10.1039/D0TB00472C (IF: 5.344)

The effect of solvent quality on pathway-dependent solution-state self-assembly of an amphiphilic diblock copolymer

S. Venkataraman, G. Wei, K. P. Mineart, J. L Hedrick, V. M. Prabhu, Y. Y. Yang
Journal of Applied Physics 127 (2020) 125104 (IF: 2.367)

BCL-xL/BCL2L1 is a Critical Anti-Apoptotic Protein that Promotes the Survival of Differentiating Pancreatic Cells from Human Pluripotent Stem Cells

L. S. W. Loo, A. A. P. Soetedjo, H. H. Lau, N. H. J. Ng, S. Ghosh, L. Nguyen, V. G. Krishnan, H. Choi, X. Roca, S. Hoon and A. K. K. Teo
Cell Death & Disease, 11 (2020), 378

Synthetic Macromolecules as Therapeutics that Overcome Resistance in Cancer and Microbial Infection

J. Tan, J. Tay, J. Hedrick and Y. Y. Yang
Biomaterials, 2020; DOI: 10.1016/j.biomaterials.2020.120078 (IF: 10.317)

Surface Tethering of InflammationModulatory Nanostimulators to Stem Cells for Ischemic Muscle Repair

J. Leong, Y.-T. Hong, Y.-F. Wu, E. Ko, S. Dvoretskiy, J. Y. Teo, B. S. Kim, K. Kim, H. Jeon, M. Boppart, Y. Y. Yang and H. Kong
ACS Nano 14 (2020) 5298-5313; DOI: 10.1021/acsnano.9b04926  (IF: 14.588)

Identification of Malassezia furfur Secreted Aspartyl Protease 1 (MfSAP1) and Its Role in Extracellular Matrix Degradation

S. E. Poh, J. P. Z. Goh, C. Fan, W. Chua, S. Q. Gan, P. L. K. Lim, B. Sharma, D. I. Leavesley, T. L. Dawson Jr. and H. Li
Frontiers in Cellular and Infection Microbiology (2020), DOI: 10.3389/fcimb.2020.00148

Early Fate Defines Microglia and Non-parenchymal Brain Macrophage Development

S. G. Utz, P. See, W. Mildenberger, M. S. Thion, A. Silvin, M. Lutz, F. Ingelfinger, N. A. Rayan, I. Lelios, A. Buttgereit, K. Asano, S. Prabhakar, S. Garel, B. Becher, F. Ginhoux and M. Greter
Cell, 181 (2020),1-17

A Two-Pronged Anti-Leukemic Agent Based on a Hyaluronic Acid–Green Tea Catechin Conjugate for Inducing Targeted Cell Death and Terminal Differentiation

K. Liang, K. H. Bae, A. Nambu, B. Dutta, J. E. Chung, M. Osato and M. Kurisawa
Biomaterials Science, 8 (2020), 497-505

Glycine Enhances Satellite Cell Proliferation, Cell Transplantation, and Oligonucleotide Efficacy in Dystrophic Muscle

Lin C, Han G, Ning H, Song J, Ran N, Yi X, Seow Y, Yin H.
Mol Ther. 2020 May 6;28(5):1339-1358. doi: 10.1016/j.ymthe.2020.03.003. Epub 2020 Mar 10.

Identification and engineering of 32 membered antifungal macrolactone notonesomycins

Goh F, Zhang MM, Lim TR, Low KN, Nge CE, Heng E, Yeo WL, Sirota FL, Crasta S, Tan Z, Ng V, Leong CY, Zhang H, Lezhava A, Chen SL, Hoon SS, Eisenhaber F, Eisenhaber B, Kanagasundaram Y, Wong FT, Ng SB.
Microb Cell Fact. 2020 Jan 6;19(1):3. doi: 10.1186/s12934-019-1274-y.

Biosynthetic engineering of the antifungal, anti-MRSA auroramycin

Yeo WL, Heng E, Tan LL, Lim YW, Ching KC, Tsai DJ, Jhang YW, Lauderdale TL, Shia KS, Zhao H, Ang EL, Zhang MM, Lim YH, Wong FT.
Microb Cell Fact. 2020 Jan 6;19(1):3. doi: 10.1186/s12934-019-1274-y.

Trxlp, a thioredoxin-like effector from Edwardsiella piscicida inhibits cellular redox signaling and nuclear translocation of NF-κB

Sayed A*, Chakraborty S*, Leung KY, Sugii S, Mok YK
Int J Biol Macromol, (2020) DOI: 10.1016/j.ijbiomac.2020.01.114.