Biography
Yaw Shin received his B.Sc. and M.Sc. degrees from the University of Malaya, Malaysia. He later earned his M.S. and Ph.D. degrees from Albert Einstein College of Medicine in New York under the tutelage of Margaret Kielian, discovering novel host factors that impact alphavirus (e.g., Chikungunya virus, Semliki Forest virus) entry and exit. After that, he pursued his postdoctoral research in the laboratory of Jan Carette at Stanford University School of Medicine, California, focusing on the discovery of host factors of several biomedically important viruses, such as flaviviruses (e.g., Dengue virus, Zika virus) and enteroviruses (e.g., Enterovirus D68, Rhinovirus C). In late 2019, he joined the Emerging Infectious Diseases (EID) Program of Duke-NUS Medical School as a tenure-track assistant professor. Yaw Shin has a long-established interest in the discovery and mechanistic studies of host genetic determinants essential for RNA virus infections using functional genomic approaches. Through collaborative research efforts, he has contributed to the discovery of several key host factors, such as TSPAN9, which facilitates alphavirus membrane fusion, SETD3, which governs enterovirus replication and pathogenesis, and a catalog of the endoplasmic reticulum-associated proteins, e.g., Vigilin, RRBP1, TMEM41B, and VMP1, that are essential for flavivirus infection. Yaw Shin's lab research focus has centered on the molecular interplay between host factors and bat- and mosquito-borne viruses of medical importance, with the hope to reveal Achille’s heels of these viruses that can be targeted for therapeutic interventions.
Publications
**Ooi, Y.S., **Stiles, K., **Liu, C.Y., Taylor, G.M., and Kielian, M. (2013). Genome-wide RNAi screen identifies novel host proteins required for alphavirus entry. PLoS Pathogens 9(12):e1003835. **co-first authors.Ooi, Y.S., Dubé, M., and Kielian, M. (2015). BST2/Tetherin inhibits alphavirus exit. Viruses 7(4):2147-2167.**Marceau, C.D., **Puschnik, A.S., Majzoub, K., Ooi, Y.S., Brewer, S.M., Fuchs, G., Swaminathan, K., Mata, M.A., Elias, J.E, Sarnow, P., and Carette, J.E. (2016). Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535(7610):159-63. **co-first authors.**Li, B., **Ding, S., Feng, N., Mooney, N., Ooi, Y.S., Ren, L., Diep, J., Kelly, M.R., Yasukawa, L.L., Patton, J.T., Yamazaki, H., Shirao, T., Jackson, P.K., and Greenberg, H.B. (2017). Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis. PNAS 114(18):E3642-E3651. **co-first authors.Puschnik, A.S., Marceau, C.D., Ooi, Y.S., Majzoub, K., Rinis, N., Contessa, J.N., and Carette, J.E. (2017). A small molecule oligosaccharyltransferase inhibitor with pan-flaviviral activity. Cell Reports 21(11): 3032–3039.Ding, S., Diep, J., Feng, N., Ren, L., Li, B., Ooi, Y.S., Wang, X., Sanyal, M., Brulois, K.F., Kuo, C.J., Solomon, D.A., Carette, J.E., and Greenberg, H.B. (2018). STAG2 deficiency induces IFN responses via cGAS-STING pathway and restricts virus infection. Nature Communications 9(1):1485.**Wilkinson, A.W., **Diep, J., **Dai, S., Liu, S., Ooi, Y.S., Song, D., Li, T.M., Horton, J.R., Zhang, X., Liu, C., Trivedi, D.V., Ruppel, K.M., Vilches-Moure, J.G., Casey, K.M., Mak, J., Cowan, T., Elias, J.E., Nagamine, C.M., Spudich, J.A., Cheng, X., Carette, J.E., and Gozani, O. (2019). SETD3 is an actin histidine methyltransferase that prevents primary dystocia. Nature 565(7739):372-376. **co-first authors.Wan, J.J., Ooi, Y.S., and Kielian M. (2019). Mechanism of Tetherin Inhibition of Alphavirus Release. Journal of Virology 93(7). pii: e02165-18.**Ooi, Y.S., **Majzoub, K., **Flynn, R.A., Mata, M.A., Diep, J., Li, J.K., Van Buuren, N., Rumachik, N., Johnson, A.G., Puschnik, A.S., Marceau, C.D., Mlera, L., Grabowski, J.M., Kirkegaard, K., Bloom, M.E., Sarnow, P., Bertozzi, C.R., and Carette, J.E. (2019). An RNA-Centric Dissection of Host Complexes Controlling Flavivirus Infection. Nature Microbiology 4(12):2369-2382. **co-first authors.**Diep, J., **Ooi, Y.S., **Wilkinson., A.W., Peters, C.E., Foy, E., Johnson, J.R., Zengel, J., Ding, S., Weng, K.F., Laufman, O., Jang, G., Xu, J., Young, T., Verschüren, E., Kobluk, K., Elias, J.E., Sarnow, P., Greenberg, H.B., Hüttenhain, R., Nagamine, C.M., Andino, R., Krogan, N.J., Gozani, O., and Carette, J.E. (2019). Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3. Nature Microbiology 4(12):2523-2537. **co-first authors.Johnson, A.G., Flynn, R.A., Lapointe, C.P., Ooi, Y.S., Zhao, M.L., Richards, C.M., Qiao, W., Yamada, S.B., Couthouis, J., Gitler, A.D., Carette, J.E., and Puglisi, J.D. (2020). A memory of eS25 loss drives resistance phenotypes. Nucleic Acids Research 48(13):7279-7297. doi: 10.1093/nar/gkaa444.Song, Y., Hou, G., Diep, J., Ooi, Y.S., Akopyants, N.S., Beverley, S.M., Carette, J.E., Greenberg, H.B., and Ding, S. (2021). Inhibitor of growth protein 3 epigenetically silences endogenous retroviral elements and prevents innate immune activation. Nucleic Acids Research. 49(22):12706-12715.Shivaprasad, S., Weng, K.F., Ooi, Y.S., Belk, J., Carette, J.E., Flynn, R.A., Sarnow, P. (2022). Loquacious modulates flaviviral RNA replication in mosquito cells. PLoS Pathog. 18(4):e1010163.Yousefi, M., Lee, W.S., Yan, B., Cui, L,Yong, C.L., Yap, X., Tay, K.S.L., Qiao, W., Tan, D., Nurazmi, N.I., Linster, M., Smith, G.J.D., Lee, Y.H., Carette, J.E., Ooi, E.E., Chan, K.R., and Ooi, Y.S. (2022). TMEM41B and VMP1 modulate cellular lipid and energy metabolism for facilitating dengue virus infection. PLoS Pathog. (in press).png?sfvrsn=b11c2136_5)
