Aneuploidy and Genome Instability

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Giulia Rancati

Giulia Rancati is a cell and evolutionary biologist. She is Senior Principal Investigator at the Institute of Medical Biology, A*STAR and Principal Investigator at the Biotransformation Platform for Innovation, A*STAR, Singapore. In 2011 she was awarded the A*STAR Investigatorship and in 2017 she become an EMBO Young Investigator. Among her scientific contributions, she showed how eukaryotic cells adaptively evolve in response to extreme genetic perturbations and the consequences of large-scale genome changes on the cellular phenotype.

Internships available: Does aneuploidy increase cellular evolvability? Read more here.

We are fascinated by evolvability, which is the remarkable capacity of cells to adapt to a variety of hostile conditions. In out lab we combine cell and systems biology approaches to understand cellular evolvability in mechanistic terms using yeast, mammalian and mouse model systems.  We are currently focusing our efforts in dissecting mechanisms of adaptation in response to harsh environmental or genetic perturbations and the role of cellular stresses in promoting genome instability. This research programme has important implications for our understanding of the design principles of cellular systems as well as for both medical and synthetic biology applications.


Internships available: Does aneuploidy increase cellular evolvability? Read more here.

Group Members

Research Fellows Kathy LARRIMORE

Michael MAIER
Postgraduate Students   Altea TARGA 
   Federica NATALI
Research Officers CHAN Yong Jie, Andrew 
  WONG Cheng Kit
 Interns YONG Luo Yan 
  FUNG Wing Hong, Ronald



Tan Z, Chu ZV, Chan YJA, Lu YE, Rancati G. Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Scientific Reports. 2018 Feb 13;8(1):2890. doi:10.1038/s41598-018-20186-7 Link
Targa A, Rancati G. Cancer: a CINful evolution. Curr Opin Cell Biol. 2018 Jun;52:136-144. doi: 10.1016/ Link
He Q, Au B, Kulkarni M, Shen Y, Lim KJ, Maimaiti J, Wong CK, Luijten MNH, Chong HC, Lim EH, Rancati G, Sinha I, Fu Z, Wang X, Connolly JE, Crasta KC. Chromosomal instability-induced senescence potentiates cell non-autonomous tumourigenic effects. Oncogenesis. 2018 Aug 15;7(8):62. doi: 10.1038/s41389-018-0072-4 Link
Tamborrini D, Juanes MA, Ibanes S, Rancati G, Piatti S. Recruitment of the mitotic exit network to yeast centrosomes couples septin displacement to actomyosin constriction. Nat Commun. 2018 Oct 17;9(1):4308. doi: 10.1038/s41467-018-06767-0 Link
Trott, J, Ee KT, Ong S, Titmarsh DM, Denil SLIJ, Giam M, Wong CK, Wang J, Shboul M, Eio M, Cooper-White J, Cool SM, Rancati G, Stanton LW, Reversade R, Dunn NR. Long-Term Culture of Self-Renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells. Stem Cell Reports 2017 8:1675-1688. DOI: 10.1016/j.stemcr.2017.05.019 Link
Rancati G, Moffat J, Typas A, Pavelka N. Emerging and evolving concepts in gene essentiality. Nat Rev Genet. 2018 Jan;19(1):34-49. doi: 10.1038/nrg.2017.74 Link
Liu G, Rancati G. Adaptive Evolution: Don't Fix What's Broken. Curr Biol. 2016 Feb 22;26(4):R169-71. doi: 10.1016/j.cub.2015.12.029 Link
Camattari A, Goh A, Yip LY, Tan AH, Ng SW, Tran A, Liu G, Liachko I, Dunham MJ, Rancati G. Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications. Microb Cell Fact. 2016 Aug 11;15(1):139. doi: 10.1186/s12934-016-0540-5 Link
Potapova TA, Seidel CW, Box AC, Rancati G, Li R. Transcriptome analysis of tetraploid cells identifies Cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53. Mol Biol Cell. 2016 Aug 24. pii: mbc.E16-05-0268. [Epub ahead of print] Link
Giam M, Rancati G. “Aneuploidy and chromosomal instability in cancer: a jackpot to chaos.” Cell Div. 2015 May 20;10:3. doi: 10.1186/s13008-015-0009-7
Liu G, Yong MYJ, Yurieva M, Srinivasan KG, Liu J, Lim JSY, Poidinger M, Wright GD, Zolezzi F, Choi H, *Pavelka N, *Rancati G. Gene essentiality is a quantitative property linked to cellular evolvability. Cell. 2015 Dec 3;163(6):1388-99. doi: 10.1016/j.cell.2015.10.069 Link
Rancati G, Pavelka N. Karyotypic changes as drivers and catalyzers of cellular evolvability: a perspective from non-pathogenic yeasts. Semin Cell Dev Biol. 2013 Apr;24(4):332-8. doi: 10.1016/j.semcdb.2013.01.009. Epub 2013 Feb 10. Review. PubMed PMID: 23403271. Link
Zhu J, Pavelka N, Bradford WD, Rancati G, Li R. Karyotypic determinants of chromosome instability in aneuploid budding yeast. PLoS Genet. 2012;8(5):e1002719. doi: 10.1371/journal.pgen.1002719. Epub 2012 May 17. PubMed PMID: 22615582; PubMed Central PMCID: PMC3355078. Link
Pavelka N, Rancati G, Li R. Dr Jekyll and Mr Hyde: role of aneuploidy in cellular adaptation and cancer. Curr Opin Cell Biol. 2010 Dec;22(6):809-15. doi: 10.1016/ Epub 2010 Jul 23. Review. PubMed PMID: 20655187; PubMed Central PMCID: PMC2974767. Link
Eldakak A, Rancati G, Rubinstein B, Paul P, Conaway V, Li R. Asymmetrically inherited multidrug resistance transporters are recessive determinants in cellular replicative ageing. Nat Cell Biol. 2010 Aug;12(8):799-805. doi: 10.1038/ncb2085. Epub 2010 Jul 25. PubMed PMID: 20657593; PubMed Central PMCID: PMC2917193. Link
Pavelka N, Rancati G, Zhu J, Bradford WD, Saraf A, Florens L, Sanderson BW, Hattem GL, Li R. Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast. Nature. 2010 Nov 11;468(7321):321-5. doi: 10.1038/nature09529. Epub 2010 Oct 20. PubMed PMID: 20962780; PubMed Central PMCID: PMC2978756. Link
Chiroli E, Rancati G, Catusi I, Lucchini G, Piatti S. Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast. Mol Biol Cell. 2009 May;20(10):2626-37. doi: 10.1091/mbc.E08-11-1150. Epub 2009 Apr 1. PubMed PMID: 19339280; PubMed Central PMCID: PMC2682603. Link
Rancati G, Pavelka N, Fleharty B, Noll A, Trimble R, Walton K, Perera A, Staehling-Hampton K, Seidel CW, Li R. Aneuploidy underlies rapid adaptive evolution of yeast cells deprived of a conserved cytokinesis motor. Cell. 2008 Nov 28;135(5):879-93. doi: 10.1016/j.cell.2008.09.039. PubMed PMID: 19041751; PubMed Central PMCID: PMC2776776. Link
Rancati G, Li R. Polarized cell growth: double grip by CDK1. Curr Biol. 2007 Aug 7;17(15):R600-3. PubMed PMID: 17686432. Link
Mapelli M, Filipp FV, Rancati G, Massimiliano L, Nezi L, Stier G, Hagan RS, Confalonieri S, Piatti S, Sattler M, Musacchio A. Determinants of conformational dimerization of Mad2 and its inhibition by p31comet. EMBO J. 2006 Mar 22;25(6):1273-84. Epub 2006 Mar 9. PubMed PMID: 16525508; PubMed Central PMCID: PMC1422169. Link
Nezi L, Rancati G, De Antoni A, Pasqualato S, Piatti S, Musacchio A. Accumulation of Mad2-Cdc20 complex during spindle checkpoint activation requires binding of open and closed conformers of Mad2 in Saccharomyces cerevisiae. J Cell Biol. 2006 Jul 3;174(1):39-51. PubMed PMID: 16818718; PubMed Central PMCID: PMC2064158. Link
Rancati G, Crispo V, Lucchini G, Piatti S. Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast. Cell Cycle. 2005 Jul;4(7):972-80. Epub 2005 Jul 9. PubMed PMID: 15970700. Link