Weiping HAN

Metabolism in Human Diseases

Profile

Weiping HAN

Deputy Director, SBIC
Head, Laboratory of Metabolic Medicine, SBIC

Research Director, MHDU, IMCB
Professor, Department of Biochemistry, YLL School of Medicine, National University of Singapore
Professor, Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School

Weiping Han obtained his Ph.D. in Physiology from Cornell University in 1996. He did his postdoctoral work at the University of Pittsburgh and HHMI/UT Southwestern Medical Center in Dallas. In 2003, he was promoted to Research Assistant Professor in the Center for Basic Neuroscience at UT Southwestern Medical Center, where he studied molecular mechanisms of hormone secretion and signaling. In 2005, he moved to Singapore to set up a research program in the Laboratory of Metabolic Medicine (LMM) at Singapore Bioimaging Consortium (SBIC), and was promoted to Research Director / Professor in 2013. Currently he is Deputy Director of SBIC with concurrent appointment as Head of LMM. He is also Research Director at Institute of Molecular and Cell Biology; Professor in the Department of Biochemistry at National University of Singapore and Program in Cardiovascular and Metabolic Disorders, Duke-NUS.

Research

Metabolism in Human Diseases Unit

Research interests
My research aims to understand the molecular basis of diabetes and its complications, to discover and validate targets for drug development, and to develop animal models for evaluation of therapeutic interventions. My lab uses molecular genetics, cell biology, and physiology techniques to analyze genetically modified animals and cell lines. The current major research projects are:

  • Molecular control of regulated exocytosis in diabetes development and treatment
  • Molecular and cellular mechanisms of adipogenesis and its link to diabetes and other metabolic diseases
  • Post-translational modifications and protein functions in hormone secretion and diabetes development
  • Molecular link underlying increased risk of cognitive impairments in people with diabetes and other metabolic diseases

Staff

Department: Weiping HAN

Name: Alice Kin Yui LO

Designation: Research Fellow

Email: kylo@imcb.a-star.edu.sg


Publications

Recent Publications

SNg Y, Tan S, Chia SY, Sun L, Hong W, Han W:
HoxC10 suppresses browning of white adipose tissue.
Experimental & Molecular Medicine 49(2):e292, February 10, 2017.

Ulyanova A, To XV, Asad AB, Han W, Chuang KH:
 MEMRI detects neuronal activity and connectivity in hypothalamic neural circuit responding to leptin.
Neuroimage: S1053-8119(16):30559-6, October 8, 2016.

Mao L, Nie B, Nie T, Hui X, Gao X, Lin X, Liu X, Xu Y, Tang X, Yuan R, Li K, Li P, Ding K, Wang Y, Xu A, Fei J, Han W, Liu P, Madsen L, Kristiansen K, Zhou Z, Ding S, Wu D:
Visualization and quantification of browning using a UCP1-2A-Luciferase knock-in mouse model.
Diabetes66(2):407-417, February, 2017.

Sorrenson B, Cognard E, Lee KL, Dissanayake WC, Fu Y, Han W, Hughes WE, Shepherd PR: A critical role for b-catenin in modulating levels of insulin secretion from b-cells by regulating actin cytoskeleton and insulin vesicle localization.
The Journal of Biological Chemistry 291(50):25888-25900, December 9, 2016.

Liu T, Li H, Hong W, Han W:
BIG3 is localized in lysosomes and regulates inhibitory synaptic transmission in hippocampal neurons.
The Journal of Neurochemistry139(5):748-756, December, 2016.

Li H, Kek HC, Lim J, Gelling RW, Han W:
Green tea (-)-epigallocatechin-3-gallate corrects high-fat diet induced feeding disorders in mice. Molecular Nutrition and Food Research60(12):2565-2575, December, 2016.

Nie T, Hui X, Mao L, Nie B, Li K, Sun W, Gao X, Tang X, Xu Y, Jiang B, Tu Z, Li P, Ding K, Han W, Zhang S, Xu A, Ding S, Liu P, Patterson A, Cooper G, Wu D:
Harmine induces adipocyte thermogenesis through RAC1-MEK-ERK-CHD4 axis.
Scientific Reports6:36382, November 2, 2016.

Chen N, Sugihara H, Kim J, Fu Z, Barak B, Sur M, Feng G*, Han W*:
Direct modulation of GFAP-expressing glia in the Arcuate nucleus bi-directionally regulates feeding.
E-Life 5:e18716, October 18, 2016. (*Co-corresponding authors)

Lim YC, Chia SY, Jin S, Han W, Ding C, Sun L:
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis.
Molecular Metabolism 5(10):1033-1041, August 17, 2016.

Kim S-Y, Sim CK, Zhang Q, Tang H, Brunmeir R, Pan H, Karnani N, Han W, Zhang K, Xu F:
An alternative strategy for pan-acetyl-lysine antibody generation.
PLoS One 11(9):e0162528, September 8, 2016.

Bai B, Man AW, Yang K, Guo Y, Xu C, Tse HF, Han W, Bloksgaard M, De Mey JG, Vanhoutte PM, Xu A, Wang Y:
Endothelial SIRT1 prevents adverse arterial remodeling by facilitating HERC2-mediated degradation of acetylated LKB1.
Oncotarget 7(26):39065-39081, June 28, 2016.

Kim S-Y, Sim KC, Tang H, Han W, Zhang K, Xu F:
Acetylome study in mouse adipocytes identifies targets of SIRT1 deacetylation in chromatin organization and RNA processing.
Archives of Biochemistry and Biophysics 598:1-10, 2016.

Takeda K, Sriram S, Chan DXH, Ong WK, Yeo CR, Tan B, Lee SA, Kong KV, Hoon S, Jiang H, Yuen JJ, Perumal J, Agrawal M, Vaz C, So J, Shabbir A, Blaner WS, Olivo M, Han W, Tanavde V, Toh SA, Sugii S:
Retinoic acid mediates visceral-specific adipogenic defects of human adipose-derived stem cells.
Diabetes65(5):1164-1178, 2016.

Wang C, Wang Y, Hu M, Han W, Zhang CX, Zhou Z:
Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis.
EMBO Reports 17(1):47-63, 2016.

Yuan Q, Yang F, Xiao Y, Tan S, Husain N, Ren M, Hu Z, Martinowich K, Ng JS, Kim PJ, Han W, Nagata KI, Weinberger DR, Je HS:
Regulation of Brian-Derived Neurotrophic Factor exocytosis and Gamma-Aminobutyric Acidergic interneuron synapse by the schizophrenia susceptibility gene dysbindin-1.
Biological Psychology 80(4):312-322, 2016.

Kim ES, Samanta A, Cheng HS, Ding Z, Han W, Toschi L, Chang YT:
Effect of oncogene activating mutations and kinase inhibitors on amino acid metabolism of human isogenic breast cancer cells.
Molecular BioSystems11(12):3378-3386, 2015.

Lim CY, Han W:
Tropomodulin 3 as the link between insulin-activated AKT2 and cortical actin remodeling in preparation of GLUT4 exocytosis.
BioArchitecture 4(6):210-214, 2015.

Kim S-Y, Sim CK, Tang H, Han W, Zhang K, Xu F:
Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver.
PLoS ONE 10(10):e0140619, October 15, 2015.

Lim CY, Hong W, Han W:
Adiponectin is stored and released through a regulated mechanism in adipocytes.
Biochemical Journal471(3):381-389, 2015.

Wu B, Wei S, Petersen N, Ali Y, Wang X, Rorsman P, Hong W, Südhof TC*, Han W*:
Synaptotagmin-7 is a GLP-1 effector in its potentiation of glucose-stimulated insulin secretion.
Proceedings of the National Academy of Sciences of the United States of America
112(32):9996-10001, 2015. (*co-corresponding authors)

Liu Y*, Li H*, Sugiura Y*, Han W*, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W:
Ubiquitin-synaptobrevin fusion protein causes degeneration of presynaptic motor terminals in mice.
The Journal of Neuroscience35(33):11514-11531, 2015. (*co-first author)

Qiu B, Shi X, Zhou Q, Chen H, Lim J, Han W*, Tergaonkar V*:
Hypothalamic NUCKS regulates peripheral glucose homeostasis.
Biochemical Journal469(3):391-398, 2015. (*co-corresponding authors)

Kim S-Y, Zhang Q, Brunmeir R, Han W, Xu F:
SIRT1 interacts with and deacetylates ATP6V1B2 in mature adipocytes.
PLoS ONE 10(7):e0133448, 2015.

Kee AJ, Yang L, Lucas CA, Greenberg MJ, Martel N, Leong GM, Hughes WE, Cooney GJ, James DE, Ostap EM, Han W, Gunning PW, Hardeman EC:
An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake.
Traffic
16(7):691-711, 2015.

Nie T, Hui X, Gao X, Nie B, Mao L, Tang X, Yuan R, Li K, Li P, Xu A, Liu P, Ding S, Han W, Cooper G, Wu D:
Direct conversion of non-adipogenic fibroblasts into adipocytes by a defined hormone mixture.
Biochemical Journal467(3):487-494, 2015.

Kim JI, Huh JY, Sohn JH, Choe SS, Lee YS, Lim CY, Jo A, Park SB, Han W, Kim JB:
Lipid-overloaded enlarged adipocytes provoke insulin resistance independent of inflammation.
Molecular and Cell Biology 35(10):1686-1699, 2015.

Li H, Liu T, Lim J, Gounko NV, Hong W, Han W:
Increased biogenesis of glucagon-containing secretory granules and glucagon secretion in BIG3-knockout mice.
Molecular Metabolism4(3):246-252, 2015.

Xu P, Wu J, Brunmeir R, Kim S, Zhang Q, Ding C, Han W, Xie W, Xu F:
TELP: a sensitive and versatile library construction method for next-generation sequencing.
Nucleic Acid Research43(6):e35, 2015.

Ma W, Fuentes G, Verma C, Radda GK, Han W:
FoxO1 negatively regulates leptin-induced POMC transcription through direct interaction with STAT3.
Biochemical Journal466(2):291-298, 2015.

Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W:
Tropomodulin 3 is a novel Akt2 effector regulating GLUT4 exocytosis.
Nature Communications6:5951, 2015.

Ramlee MK, Zhang Q, Idris M, Peng X, Sim CK, Han W, Xu F:
Histone H3 K27 acetylation marks a potent enhancer for the adipogenic master regulator gene Pparg2.
Cell Cycle13(21):3414-3422, 2014.

Yang W, Thein S, Lim C-Y, Ericksen RE, Sugii S, Xu F, Robinson RC, Kim JB, Han W:
Arp2/3 complex regulates adipogenesis by controlling cortical actin remodeling.
Biochemical Journal464(2):179-192, 2014.

Liu T, Li H, Gounko NV, Zhou Z, Xu A, Hong W, Han W:
Increased insulin granule biogenesis and enlarged releasable pool of insulin granules support enhanced insulin secretion in BIG3 knockout mice.
The American Journal of Physiology Endocrinology and Metabolism 307(7):E611-618, 2014.

Hornyak I, Madacsi E, Kalugyer P, Vacz G, Horvathy DB, Szendroi M, Han W, Lacza Z: Increasing release time of antibiotics from bone allografts through a novel biodegradable coating.
Biomed Research International 2014:459867/8 pages, 2014.

Smith GC, Zhang ZY, Mulvey T, Lach S, Xiu P, Petersen N, Han W, Phillips A, Wang MW, Shepherd PR:
Clozapine directly increases insulin and glucagon secretion from islets: Implications for impairment of glucose tolerance.
Schizophrenia Research S0920-9964(14)00230-8, June 3, 2014.

Qiu B, Shi X, Wong ET, Lim J, Bezzi M, Low D, Zhou Q, Akincilar SC, Lakshmanan M, Swa HL, Tham JM, Gunaratne J, Cheng KK, Hong W, Lam KS, Ikawa M, Guccione E, Xu A, Han W*, Tergaonkar V*:
NUCKS is a positive transcriptional regulator of insulin signaling.
Cell Reports 7(6):1876-1886, 2014. (*co-corresponding authors)

Li H, Wei S, Cheng K, Gounko NV, Ericksen RE, Xu A, Hong W*, Han W*:
BIG3 inhibits insulin granule biogenesis and insulin secretion.
The EMBO Reports 15(6):714-722, 2014. (*co-corresponding authors)

Ericksen RE, Rose S, Westphalen CB, Shibata W, Muthupalani S, Tailor Y, Han W, Fox JG, Ferrante AW, Wang TC:
Obesity accelerates Helicobacter felis-induced gastric carcinogenesis by enhancing immature myeloid cell trafficking and TH17 response.
GUT 63(3):385-394, 2014.

Wang Y, Ali Y, Lim CY, Hong W, Pang Z*, Han W*:
Insulin-stimulated leptin secretion requires calcium and PI3K-Akt activation.
Biochemical Journal458(3):491-498, 2014. (*co-corresponding authors)

Ong WK, Tan CS, Chan KL, Goesantoso GG, Chan XHD, Chan E, Yin J, Yeo CR, Khoo CM, So J, Shabbir A, Toh S-A, Han W, Sugii S:
Identification of specific surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots.
Stem Cell Reports 2(2):171-179, 2014.

Wei S, Soh S, Qiu W, Yang W, Guo J, Ong W-Y, Pang Z*, Han W*:
Seipin with motor neuropathy-associated mutations impairs neurotransmission in cortical neurons.
Journal of Neurochemistry 129(2):328-338, 2014. (*co-corresponding authors)

Wang X, Lacza Z, Sun YE, Han W:
Leptin resistance and obesity in mice with deletion of MeCP2 in hypothalamic POMC neurons.
Diabetologia57(1):236-245, 2014.

Yang W, Thein S, Wang X, Bi X, Ericksen RE, Xu F, Han W:
BSCL2/Seipin regulates adipogenesis through actin cytoskeleton remodeling.
Human Molecular Genetics 23(2):502-513, 2014.