Shi Yan NG

Neurotherapeutics Laboratory

Profile

Shi Yan NG
Lab Location: #5-07   Email: syng@imcb.a-star.edu.sg   Tel: 65869525

Dr. Shi Yan Ng completed her Bachelor in Science with First Class Honours at the National University of Singapore (NUS) and her Ph.D with Dr. Lawrence Stanton at the Genome Institute of Singapore, under the NUS Graduate School (NGSS) scholarship. With a keen interest in stem cells and modeling neurological disease, Shi Yan joined Professor Lee Rubin’s laboratory at Harvard University to study Spinal Muscular Atrophy, an inherited motor neuron disease affecting children. Thereafter, Shi Yan began her independent research at IMCB in 2015, where she continued to study mechanisms of neurological diseases using patient-derived induced pluripotent stem cells. Shi Yan has won several fellowships and awards for her work, including the National Research Foundation (NRF) Fellowship (Class of 2018), the A*STAR International Fellowship (2012), and the Merck Millipore Young Scientist Award (2011). She is currently a Principal Investigator at IMCB, and an Assistant Professor at Department of Physiology, NUS Medicine.

staff

Department: Shi Yan NG

Name: Munirah Md SANTOSA

Designation: Research Officer

Email: munirahs@imcb.a-star.edu.sg


Name: Jin Hui HOR

Designation: PhD Student

Email: jhhor@imcb.a-star.edu.sg


Name: Li Yi TAN

Designation: Research Officer

Email: tanly@imcb.a-star.edu.sg


Name: Winanto NG

Designation: Research Officer

Email: winanto@imcb.a-star.edu.sg


Name: Valerie Jing Wen LIM

Designation: Research Officer

Email: limjwv@imcb.a-star.edu.sg


Name: Sandhya PRAKASH KAMATH

Designation: Research Fellow

Email: sandhyapk@imcb.a-star.edu.sg


Name: Zheng Shan CHONG

Designation: Research Fellow

Email: chongzs@imcb.a-star.edu.sg


Name: Shi Yan NG

Designation: Independent Fellow

Email: syng@imcb.a-star.edu.sg


Name: Yong Hui KOH

Designation: PhD Student

Email: yhkoh@student.imcb.a-star.edu.sg


Research

Neurotherapeutics Laboratory

Our team is interested in dissecting mechanisms of neurodegeneration and neural dysfunctions using human induced pluripotent stem cells.

1. Altered metabolism in neurodegeneration
Neurons rely on oxidative phosphorylation for their energetic requirements, and motor neurons are particularly active cells that synapse with skeletal muscles to control voluntary movement. In Amyotrop


2. Spinal organoid models of motor neuron diseases
Using our unique spinal organoid cultures, we are starting to understand molecular differences between the conventional 2D and 3D organoid cultures. In vivo, spinal cords are patterned from rostral (head) to caudal (tail). Our 2D protocols primary generate motor neurons of a rostral identity while the organoid cultures produce motor neurons of a caudal identity. Using single cell RNA-seq, we aim to understand the molecular basis of organization that defines organoid cultures.


3. Astrocytes in neurodegeneration
Astrocytes are the most abundant cell type in the central nervous system, and support normal neuronal health and function by providing metabolic support, modulating synaptic transmission, and their involvement in uptake and release of factors. There is strong evidence for astrocyte (non-cell autonomous) toxicity in many neurodegenerative diseases including ALS. Our ultimate goal is to be able to decipher the basis of astrocyte toxicity and to discover therapeutics that may counter neuronal death caused by diseased astrocytes.

 

Publications

Recent Publications

Jin-Hui Hor, Eunice Shi-Yi Soh, Li Yi Tan, Valerie Jing-Wen Lim, Munirah Mohamad Santosa, Winanto, Beatrice Xuan Ho, Yong Fan, Boon-Seng Soh, Shi-Yan Ng#. 
Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy. (Under revision). 
#corresponding author

Elizabeth J. Paik*, Alison O’Neil*, Shi-Yan Ng*, Chicheng Sun, Lee L. Rubin (2018). 
Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hiPSC culture. 
Sci Rep. 8: 804 *co-first authors

Natalia Rodriguez-Muela, Nadia K. Litterman, Erika M. Norabuena, Jesse L. Mull, Maria José Galazo, Chicheng Sun, Shi-Yan Ng, Nina R. Makhortova, Andrew White, Maureen M. Lynes, Wendy K. Chung, Lance S. Davidow, Jeffrey D. Macklis, Lee L. Rubin (2017). 
Single-cell analysis of SMN reveals its broader role in neuromuscular disease. 
Cell Rep. 18: 1484-1498.

Constantin d’Ydewalle, Daniel Ramos, Noah J. Pyles, Shi-Yan Ng, Marius Gorz, Celeste M. Pilato, Lingling Kong, Lee L. Rubin, Frank Rigo, C. Frank Bennett, Charlotte J. Sumner (2017). 
The antisense transcript SMN-AS1 regulates SMN expression and is a novel therapeutic target for Spinal Muscular Atrophy. 
Neuron 93: 66-79. 

Mary H. Wertz, Kellen Winden, Pierre Neveu, Shi-Yan Ng, Ebru Ercan, Mustafa Sahin (2016). 
Cell type specific miR-431 dysregulation in a motor neuron model of Spinal Muscular Atrophy. 
Human Mol. Genet. 25: 2168-2181. 

Boon Seng Soh*, Shi-Yan Ng*, Hao Wu, Kristina Buac, Joo-Hye C. Park, Xiaojun Lian, Jiejia Xu, Kylie S. Foo, Ulrika Felldin, Xiaobing He, Massimo Nichane, Henry Yang, Bu Lei, Ronald A. Li, Bing Lim, Kenneth R. Chien (2016). 
Clonal human embryonic stem cell derived bi-potent cardiovascular progenitor cells generate an in vivo coronary arterial system. 
Nat. Commun. 8: 10774 *co-first authors

Samir Ounzain, Rudi Micheletti, Carme Arnan, Dario Cecchi, Isabelle Plaisance, Blanche Schroen, Michael Alexanian, Christine Gonzales, Shi-Yan Ng, Giovanni Bussoti, Iole Pezzuto, Cedric Notredame, Stephane Hetmans, Roderic Guigo, Rory Johnson, Thierry Pedrazzini (2015). 
CARMEN, a human super enhancer-associated long non-coding RNA controlling cardiac specification, differentiation and homeostasis. 
J Mol. Cell Cardiol. 89: 98-112.

Shi-Yan Ng, Boon Seng Soh, Natalia Rodriguez-Muela, David G. Hendrickson, Feodor Price, John L. Rinn, Lee L. Rubin (2015). 
Genome-wide RNA-seq of human motor neurons implicates selective ER stress activation in Spinal Muscular Atrophy. 
Cell Stem Cell 17: 569-84. 

Boon Seng Soh, Kristina Buac, Huansheng Xu, Edward Li, Shi-Yan Ng, Hao Wu, Jolanta Chmielowiec, Xin Jiang, Lei Bu, Ronald Li, Chad Cowan, Kenneth R. Chien (2014). 
N-cadherin prevents the premature differentiation of second heart field progenitors in the pharyngeal mesoderm microenvironment. 
Cell Res. 24: 1420-32.

Shi-Yan Ng, Gireesh K. Bogu, Boon Seng Soh, Lawrence W. Stanton (2013). 
The long non-coding RNA RMST interacts with SOX2 to regulate neurogenesis. 
Mol. Cell 51: 349-59.

Shi-Yan Ng, Lin Lin, Boon Seng Soh, Lawrence W. Stanton (2013). 
Long non-coding RNAs in development and disease of the central nervous system. 
Trends in Genetics 29:461-8.

Shi-Yan Ng, Lawrence W Stanton (2013). 
Long non-coding RNAs in stem cell pluripotency. 
Wiley Interdiscip.Rev RNA 4: 121-8.

Boon Seng Soh, Dahai Zheng, Julie Su Li Yeo, Henry He Yang, Shi-Yan Ng, Lan Hiong Wong, Wencai Zhang, Pin Li, Massimo Nichane, Atasha Asmat et al. (2012). 
CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury. 
Mol. Ther. 20: 2335-46.

Shi-Yan Ng, Rory Johnson, Lawrence W. Stanton (2012). 
Human long non-coding RNAs promote pluripotency and neuronal differentiation by association with chromatin modifiers and transcription factors. 
EMBO J.  31: 522-533. 

Xu Jiang, Hao Qing Cao, Li Ya Shi, Shi-Yan Ng, Lawrence W. Stanton, Sing Yian Chew (2012). 
Nanofiber topography and sustained biochemical signaling enhance human mesenchymal stem cell neural commitment. 
Acta Biomater. 8: 1290-1302.


BOOK CHAPTERS
________________________________________________________________
Shi-Yan Ng and Lee L. Rubin (2016). 
Cell Culture Models of Spinal Muscular Atrophy. In C. J. Sumner, S. Paushkin, and C.-P. Ko (Eds), 
Spinal Muscular Atrophy: Disease Mechanisms and Therapy