Qingfeng CHEN

Humanized Mouse Unit (HMU)

Profile

Qingfeng CHEN
Lab Location: #5-15B   Email: qchen@imcb.a-star.edu.sg   Tel: 65869873


Qingfeng Chen received his B.S. in 2003 and Ph.D in June 2008 from University of Science and Technology of China. In July 2008, he joined in Singapore-MIT Alliance for Research and Technology (SMART) to pursue postdoctoral research on developing humanized mouse models. In 2010, he was promoted as research scientist in SMART. He Joined in Institute of Molecular and Cell Biology, A*STAR as a group leader in January 2012 to establish humanized mouse unit. He is currently a Principal Investigator in IMCB, joint Principal Investigator in National Cancer Centre Singapore and adjunct assistant professor in Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore. Qingfeng Chen is also an awardee of Gilead Sciences International Research Scholar and Singapore National Research Foundation Fellowship. His research interest is developing humanized mouse models for the study of human infectious diseases, cancers, autoimmune diseases and stem cells.

Research


Humanized Mouse Unit (HMU)


Humanized mouse refers to mouse stably reconstituted with human cells and tissues. Our mission is to develop humanized mouse models to closely recapitulate in vivo human biology to study human diseases and drug development.
Currently 
our research areas cover:



Humanized Mouse Unit (HMU)
Humanized Mouse Unit (HMU)

Staff

Department: Qingfeng CHEN

Name: Yue ZHAO

Designation: Senior Research Fellow

Email: yzhao@imcb.a-star.edu.sg


Name: Kylie Su Mei YONG

Designation: Research Fellow

Email: yongsmk@imcb.a-star.edu.sg


Name: Jia Ying CHENG

Designation: Research Officer

Email: chengjy@imcb.a-star.edu.sg


Name: Simon Wai Nam LIU

Designation: Research Fellow

Email: wnliu@imcb.a-star.edu.sg


Name: Sue Yee TAN

Designation: Laboratory Officer

Email: sytan@imcb.a-star.edu.sg


Name: Wilson Wei Sheng TAN

Designation: Research Officer

Email: wstan@imcb.a-star.edu.sg


Name: Shin Yie FONG

Designation: Laboratory Officer

Email: fongsy@imcb.a-star.edu.sg


Name: Fritz Shien Choong LAI

Designation: Collaborator

Email: sclai@imcb.a-star.edu.sg


Name: Ying Ying HEY

Designation: Research Fellow

Email: heyyy@imcb.a-star.edu.sg


Name: Zhisheng HER

Designation: Research Fellow

Email: zsher@imcb.a-star.edu.sg


Publications

Kylie Su Mei Yong, Justin Han Jia Ng, Zhisheng Her, Sue Yee Tan, Wilson Wei Sheng Tan, Ying Ying Hey, Sergio Erdal Irac, Min Liu, Xue Ying Chan, Merry Gunawan, Randy Jee Hiang Foo, Dolyce Hong Wen Low, Ian Hewitt Mendenhall, Yok Teng Chionh, Charles-Antoine Dutertre, Qingfeng Chen#, Lin-Fa Wang#.
Bat-mouse bone marrow chimera: a novel animal model for dissecting the uniqueness of the bat immune system.
Scientific Reports, In press.

Nathaniel H. Park, Wei Cheng, Fritz Lai, Chuan Yang, Paola Florez de Sessions, Balamurugan Periaswamy, Collins Wenhan Chu, Simone Bianco, Shaoqiong Liu, Shrinivas Venkataraman, Qingfeng Chen#, Yi Yan Yang, and James L Hedrick.
Addressing Drug Resistance in Cancer with Macromolecular Chemotherapeutic Agents.
J. Am. Chem. Soc. March 5, 2018. DOI: 10.1021/jacs.7b11468. (IF = 13.858)

Lay Teng Ang, Antson Kiat Yee Tan, Matias Ilmari Autio, Joanne Su Hua Goh, Siew Hua Choo, Kian Leong Lee, Jianmin Tan, Bangfen Pan, Jane Jia Hui Lee, Jen Jen Lum, Christina Ying Yan Lim, Kai Xin Yeo, Jin Yee Wong, Min Liu, Ling Li Oh, Pei Lynn Chia, Chet Hong, Loh, Angela Chen, Qingfeng Chen, Irving L. Weissman, Kyle M. Loh & Bing Lim.
A roadmap for human liver differentiation from pluripotent stem cells.
Cell Reports. Volume 22, Issue 8, p2190–2205, 20 February 2018. (IF = 8.282)

Kylie Su Mei Yong, Zhisheng Her, Qingfeng Chen#.
Humanized mice as unique tools for human-specific studies.
Archivum Immunologiae et Therapiae Experimentalis. 07 February 2018. (IF = 3.176)

Yi Yang, Han Wu, Xiangjin Kang, Yanhui Liang, Ting Lan, Tianjie Li, Tao Tan, Jiangyun Peng, Quanjun Zhang, Geng An, Yali Liu, Qian Yu, Zhenglai Ma, Ying Lian, Boon Seng Soh, Qingfeng Chen, Ping Liu, Yaoyong Chen, Xiaofang Sun, Yang Yu, Xiaoping Li, and Yong Fan.
Targeted elimination of mutant mitochondrial DNA in MELAS iPSCs by mitoTALENs.
Protein & Cell. 09 January 2018. (IF = 5.374)

Merry Gunawan, Zhisheng Her, Min Liu, Sue Yee Tan, Xue Ying Chan, Wilson Wei Sheng Tan, Shubasree Dharmaraaja, Yong Fan, Chee Bing Ong, Eva Loh, Kenneth Tou En Chang, Thiam Chye Tan, Jerry Kok Yen Chan, Qingfeng Chen#.
A Novel Human Systemic Lupus Erythematosus Model in Humanised Mice.
Scientific Reports. Nov 30, 2017. doi:10.1038/s41598-017-16999-7.

Yi Yang, Han Wu, Xiangjin Kang, Yanhui Liang, Ting Lan, Tianjie Li, Tao Tan, Jiangyun Peng, Quanjun Zhang, Geng An, Yali Liu, Qian Yu, Zhenglai Ma, Ying Lian, Boon Seng Soh, Qingfeng Chen, Ping Liu, Yaoyong Chen, Xiaofang Sun, Yang Yu, Xiaoping Li, and Yong Fan.
Targeted elimination of mutant mitochondrial DNA in MELAS iPSCs by mitoTALENs.
Protein & Cell. In press

Her Z, Yong KSM, Paramasivam K, Tan WWS, Chan XY, Tan SY, Liu M, Fan Y, Linn YC, Hui KM, Surana U, Chen Q#.
An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia.
Journal of hematology and Oncology. Oct 6, 2017, 10:162, https://doi.org/10.1186/s13045-017-0532-x.

Zheng Z, Sze CW, Keng CT, Al-Haddawi M, Liu M, Tan SY, Kwek HL, Her Z, Chan XY, Barnwal B, Loh E, Chang KTE, Tan TC, Tan YJ, Chen Q#.
Hepatitis C virus mediated chronic inflammation and tumorigenesis in the humanised immune system and liver mouse model.
PLoS One. 2017 Sep 8;12(9):e0184127. doi: 10.1371/journal.pone.0184127. eCollection 2017.

Qingfeng Chen#.
The niche for hematopoietic stem cell expansion: a collaboration network.
Cellular & Molecular Immunology, 28 August, 2017, doi:10.1038/cmi.2017.74.

Yue Zhao, Min Liu, Xue Ying Chan, Sue Yee Tan, Sharrada Subramaniam, Yong Fan, Eva Loh, Kenneth Tou En Chang, Thiam Chye Tan, Qingfeng Chen#.
Uncovering the mystery of opposite circadian rhythms between mouse and human leukocytes in humanized mice.
Blood 30 August, 2017, blood-2017-04-778779; doi: https://doi.org/10.1182/blood-2017-04-778779.

Lay Teng Ang, Antson Tan, Matias Ilmari Autio, Joanne Su-hua Goh, Siew Hua Choo, Kian Leong Lee, Bangfen Pan, Jianmin Tan, Jane Jia Hui Lee, Isabelle Kai Xin Yeo, Chloe Jin Yee Wong, Jen Jen Lum, Chet Hong Loh, Christina Ying Yan Lim, Jueween Ling Li Oh, Cheryl Pei Lynn Chia, Angela Chen, Qingfeng Chen, Irving Weissman, Kyle M. Loh, Bing Lim.
bioRxiv August 10, 2017. doi: https://doi.org/10.1101/174698.

Vivian Vasconcelos Costa, Weijian Ye, Qingfeng Chen, Mauro Martins Teixeir, Peter Preiser, Eng Eong Ooi, Jianzhu Chen.
Dengue Virus-Infected Dendritic Cells, but Not Monocytes, Activate Natural Killer Cells through a Contact-Dependent Mechanism Involving Adhesion Molecules.
mBio, 1 August, 2017, doi: 10.1128/mBio.00741-17. vol. 8 no. 4 e00741-17.

Tan-Garcia A, Wai LE, Zheng D, Ceccarello E, Jo J, Banu N, Khakpoor A, Chia A, Tham CYL, Tan AT, Hong M, Keng CT, Rivino L, Tan KC, Lee KH, Lim SG, Newell EW, Pavelka N, Chen J, Ginhoux F, Chen Q#, Bertoletti A, Dutertre CA.
Intrahepatic CD206+ macrophages contribute to inflammation in advanced viral-related liver disease.
Journal of Hepatology, May 5. pii: S0168-8278(17)32004-4. doi: 10.1016/j.jhep.2017.04.023.

See P, Dutertre CA, Chen J, Günther P, McGovern N, Irac SE, Gunawan M, Beyer M, Händler K, Duan K, Sumatoh HRB, Ruffin N, Jouve M, Gea-Mallorquí E, Hennekam RCM, Lim T, Yip CC, Wen M, Malleret B, Low I, Shadan NB, Fen CFS, Tay A, Lum J, Zolezzi F, Larbi A, Poidinger M, Chan JKY, Chen Q, Rénia L, Haniffa M, Benaroch P, Schlitzer A, Schultze JL, Newell EW, Ginhoux F.
Mapping the human DC lineage through the integration of high-dimensional techniques.
Science, 2017, 04 May.

Liang Cui, Jue Hou, Jinling Fang, Yie Hou Lee, Vivian Costa, Lan Hiong Wong, Qingfeng Chen, Eng Eong Ooi, Steven Tannenbaum, Jianzhu Chen, and Choon Nam Ong.
Serum Metabolomics Investigation of Humanized Mouse Model with Dengue Infection.
Journal of Virology, 2017.

Dahai Zheng, Boon-Seng Soh, Lu Yin, Guangan Hu, Qingfeng Chen, Hyungwon Choi, Jongyoon Han, Vincent T. K. Chow, Jianzhu Chen.
Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro.
Scientific Reports, 2017, doi:10.1038/srep41661. (IF= 5.525)

Chong SZ, Evrard M, Devi S, Chen J, Lim JY, See P, Zhang Y, Adrover JM, Lee B, Tan L, Li J, Liong KH, Phua C, Balachander A, Boey A, Liebl D, Tan SM, Chan J, Balabanian K, Harris JE, Bianchini M, Weber C, Duchene J, Lum J, Poidinger M, Chen Q, Rénia L, Wang CI, Larbi A, Randolph GJ, Weninger W, Looney MR, Krummel MF, Biswas SK, Ginhoux F, Hidalgo A, Bachelerie F, Ng LG.
CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses.
J Exp Med. 2016 Oct 10. [Epub ahead of print]. (IF=11.24)

Xia, Jinxing, Hu, Zheng, Yoshihara, Satoshi, Li, Yuying, Jin, Chun-Hui, Tan, Shulian, Li, Wei, Chen, Qingfeng, Sykes, Megan, Yang, Yong-Guang,
Modeling human leukemia immunotherapy in humanized mice.
EBioMedicine (2016), doi:10.1016/j.ebiom.2016.06.028 Epub 2016 Jun 23.

Garnelo M, Tan A, Her Z, Yeong J, Lim CJ, Chen J, Lim KH, Weber A, Chow P, Chung A, Ooi LL, Toh HC, Heikenwalder M, Ng OL, Nardin A, Chen Q, Abastado JP, Chew V. Interaction between tumor-infiltrating B cells and T cells controls the progression of hepatocellular carcinoma.
Gut, 2015, Dec 2015, doi:10.1136/gutjnl-2015-310814. (IF=14.66).

Chen Q#, Ye W, Tan WJ, Yong K, Liu M, Tan SQ, Loh E, Chang KTE, Tan TC, Preiser PR, Chen J.
Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human.Scientific Reports, 2015, doi:10.1038/srep15118. (IF= 5.578)

L.-E. Wai, C.T. Keng, D. Zheng, A. Tan-Garcia, E. Ceccarello, C. Tham, A.T. Tanoto, A. Bertoletti, Q. Chen#.
P0483 : Changes in liver immunity upon HBV infection: a comparison of humanised mice and humans.
Journal of Hepatology, 04/2015; 62:S495. DOI:10.1016/S0168-8278(15)30691-7. (IF=10.40)

Amaladoss, A, Chen Q, Liu M, Dummler S, Dao M, Suresh S, Chen J, Preiser PR.
De Novo Generated Human Red Blood Cells in Humanized Mice Support Plasmodium falciparum Infection.
Plos One, 2015, DOI: 10.1371/journal.pone.0129825. (IF=3.234)

Yong K, Keng CT, Tan SQ, Loh E, Chang KTE, Tan TC, Hong W, Chen Q#.
Human CD34loCD133lo Fetal Liver Cells Support the Expansion of Human CD34hiCD133hi Hematopoietic Stem Cells.
Molecular and Cellular Immunology, 2015, doi:10.1038/cmi.2015.43. (IF=4.112)

Bae KH, Lee F, Xu KM, Keng CT, Tan SY, Tan YJ, Chen Q#, Kurisawa M.
Microstructured dextran hydrogels for burst-free sustained release of PEGylated protein drugs.
Biomaterials, 2015, doi:10.1016/j.biomaterials.2015.06.008. (IF=8.557)

Keng CT, Sze CW, Zheng DH, Zheng ZQ, Yong K, Tan SQ, Ong JY, Tan SY, Loh E, Upadya MH, Kuick CH, Hotta H, Lim SG, Tan TC, Chang KTE, Hong W, Chen J, Tan YJ, Chen Q#. Characterization of Liver Pathogenesis, Human Immune Responses and Drug Testing in a Humanized Mouse Model of Hepatitis C Virus Infection.
Gut. 2015, doi:10.1136/gutjnl-2014-307856. (IF=14.66)

Hong X, Nguyen HT, Chen Q, Zhang R, Hagman Z, Voorhoeve PM, Cohen SM.
Opposing activities of the Ras and Hippo pathways converge on regulation of YAP protein turnover.
EMBO J. 2014 Nov 3;33(21):2447-57. (IF=10.748)

Nguyen HT, Hong X, Tan S, Chen Q, Chan L, Fivaz M, Cohen SM, Voorhoeve PM.
Viral Small T Oncoproteins Transform Cells by Alleviating Hippo-Pathway-Mediated Inhibition of the YAP Proto-oncogene.
Cell Rep. 2014 Aug 7;8(3):707-13. (IF=7.215)

Xue C, Kwek KYC, Chan JKY, Chen Q, and Lim M.
The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells. Biotechnology Journal. 2014. Jul;9(7):980-9. (IF=3.446)

Chen Q, Amaladoss A, Ye W, Liu M, Dummler S, Kong F, Wong LH, Loo HL, Loh E, Tan SQ, Tan TC, Chang KTE, Dao M, Suresh S, Preiser PR, Chen J.
Human Natural Killer Cells Control Plasmodium falciparum Infection by Eliminating Infected Red Blood Cells.
Proc Natl Acad Sci U S A. 2014. Jan 28;111(4):1479-84. (IF=9.737)

Loh K, Ang LT, Zhang J, Kumar V, Ang J, Auyeong JQ, Lee KL, Nichane M, Poellinger L, Chen Q, Prabhakar S, Weissman IL, Lim B.
Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations.
Cell Stem Cell. 2014 Feb 6;14(2):237-52. (IF=27.361)

Sridharan A, Chen Q, Tang KF, Ooi EE, Hibberd ML, Chen J.
Inhibition of Megakaryocyte Development in the Bone Marrow Underlies Dengue Virus-induced Thrombocytopenia in Humanized Mice.
Journal of Virology. 2013 Nov;87(21):11648-58. (IF=5.076)

Li Y*, Chen Q*, Zheng D, Yin L, Chionh YH, Wong LH, Tan SQ, Tan TC, Chan JK, Alonso S, Dedon PC, Lim B, Chen J.
Induction of Functional Human Macrophages from Bone Marrow Promonocytes by M-CSF (CSF-1) in Humanized Mice.
Journal of Immunology. 2013 Sep 15;191(6):3192-9. (IF=5.520)

Chen, Q#. and Chen, J. (2013).
Serial Transfer of Human Hematopoietic and Hepatic Stem/progenitor Cells.
Bio-protocol 3(23): e992. http://www.bio-protocol.org/e992 .

Chen, Q#. and Chen, J. (2013).
Isolation of CD34+ Cells from Human Fetal Liver and Cord Blood.
Bio-protocol 3(23): e991. http://www.bio-protocol.org/e991 .

Chen Q, Khoury M, Limmon G, Choolani M, Chan JKY, Chen J.
Human Fetal Hepatic Stem Cells Are Distinct from, but Closely Related to, Hematopoietic Stem Cells.
Stem Cells. 2013 Jun;31(6):1160-9. (IF=7.9)

He F, Kiener TK, Lim XF, Tan Y, Raj KV, Tang M, Chow VT, Chen Q, Kwang J.
Development of A Sensitive and Specific Epitope-Blocking ELISA for Universal Detection of Antibodies to Human Enterovirus 71 Strains.
Plos One, 2013;8(1):e55517. (IF=3.730)

Chen Q, He F, Kwang J, Chan JKY, Chen J.
GM-CSF and IL-4 Stimulate Antibody Responses in Humanized Mice by Promoting T, B and Dendritic Cell Maturation.
Journal of Immunology. 2012 Dec 1;189(11):5223-9. (IF=5.520)

Drake A*, Chen Q*, Chen J.
Engineering Humanized Mice for Improved Hematopoietic Reconstitution.
Cell Mol Immunol. 2012 May;9(3):215-24. (IF= 3.419)

Khoury M, Drake A, Chen Q et al.
Mesenchymal Stem Cells Secreting Angiopoietin-Like-5 Support Efficient Expansion of Human Hematopoietic Stem Cells Without Compromising Their Repopulating Potential.
Stem Cells Dev. 2011 August; 20(8): 1371–1381. (IF= 4.670)

Chen Q, Khoury M, Chen J.
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.
Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21783-8. (IF=9.737)

Chen Q, Wei H, Sun R et al.
Therapeutic RNA silencing of CX3CL1 gene prevents mice from adenovirus vector-induced acute liver injury.
Hepatology. 2008 Feb;47(2):648-58. (IF=12.003)
Ren T*, Chen Q*, Tian Z, Wei H.
Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
Biochem Biophys Res Commun. 2007 Dec 28; 364(4):978-84. (IF=2.500)

Zhang X, Wei H, Chen Q et al. Activation of human natural killer cells by recombinant membrane expressed fractalkine on the surface of tumor cells.
Oncol Rep. 2007 Jun; 17(6):1371-5. (IF= 2.297)

Isolation and characteristics of murine hepatic innate lymphocytes. Z Dong, H Wei, J Wang, D Luo, L Zhang, Q Chen, Z Tian.
Academic journal of Second Military Medical College. 2001, 23 (10), 1095-1098.
Co-first author; # Corresponding author.

Patents filed:

1. Methods Of Producing Humanized Non-Human Mammals. WIPO Publication number: WO/2011/002727; Europe patent number: EP2449094. US patent number: US2012-0157667. Japan Patent Application No.: 2012-517837. China patent publication number: CN102725400A. Licensed in 2015 Feb 24th. MIT Case No. 14234D.

2. Methods Of Reconstituting Human Hepatocytes And Human Hematopoietic Cells In Non-Human Mammals. WIPO Publication number: WO/2012/005690; PCT/SG2011/000234

3. Humanized Non-Human Model. U.S. Application NO.: 61/221,438.

4. A Humanized Non-Human Mammal Model of Malaria and Uses Thereof. WIPO Publication number: WO/2012/051572; PCT/US2011/056425

5. Use of Humanized Mice to Determine Toxicity. US publication number US-2015-0007357 A1. : 61/567,466. China Patent Application No.: 2012800689704. Singapore Patent Application No. 11201402112R. EP Application No.:  12854884.9. Licensed in 2015 Feb 24th. MIT Case No. 15267D.

6. A Humanized Mouse Model For Study Of Bona Fide Hepatitis Virus Infection And Use Thereof. PCT Application number IMC/P/07941/01/PCT.

7. A chimeric animal comprising stably transplanted bat cells. ETPL Ref: IMC/Z/10031.