Small G-protein Signalling and Kinases


Lab Location: #8-15   Email: mcbmanser@imcb.a-star.edu.sg   Tel: 65869555

Ed Manser obtained his PhD (Biophysics) at National Institute for Medical Research in London working on the microtubule cytoskeleton under the supervision of Dr Peter M Bayley. He secured a postdoctoral position at Institute of Neurology, London and began looking at aspects of molecular neurobiology. As a Research Fellow at IMCB (1992) he developed an interest in the biochemical pathways downstream of the newly discovered Rho small GTPases and in 1999 was promoted to Principle Investigator, working under the GSK-IMCB Trust. He also headed the IMCB Proteomic facility 2003-2006. Currently, he holds an Associate Professor appointment, and is a Senior Principal Investigator in IMCB.


Small G-protein Signalling Kinases

Ed Manser has been working in the area of Rho Signal transduction since 1992. His team in the GSK-IMCB Research Fund has made ground-breaking discoveries in the field of signal transduction, including the first identification and isolation of effector proteins ACK, PAK, ROK, MRCK, and other associated proteins such as PIX and GIT. These have been shown to play key roles in diverse activities including shape changes, motility, differentiation and cytokinesis in many cell types, including neurons


 Recent Publications

Chan PM and Manser E. (2012)
PAKs in Human Disease
Prog Mol Biol Transl Sci. 2012;106:171-87

Lin PC, Chan PM, Hall C, Manser E. (2011)
CRMPs are a new class of MAP that selectively interact with assembled microtubules via a taxol-sensitive binding interaction.
J Biol Chem. 2011 Sep 27. [Epub ahead of print]

Prieto-Echagüe V, Chan PM, Craddock BP, Manser E, Miller WT. (2011)
PTB Domain-Directed Substrate Targeting in a Tyrosine Kinase from the Unicellular Choanoflagellate Monosiga brevicollis.
PLoS One. 2011 Apr 26;6(4):e19296.

Sit ST, Manser E. (2011)
Rho GTPases and their role in organizing the actin cytoskeleton.
J Cell Sci. 2011 Mar 1;124(Pt 5):679-83.

Chan PM (2011)
Differential signaling of Flt3 activating mutations in acute myeloid leukemia: a working mode.
Protein Cell. 2011 Feb;2(2):108-15. Epub 2011 Feb 25. Review

Chan W, Sit ST, Manser E. (2011)
The Cdc42-associated kinase ACK1 is not auto-inhibited but requires Src for activation.
Biochem J. Feb 2011

Chan PM, Ng YW and Manser E. (2010)
A robust protocol to map binding sites of the 14-3-3 interactome: Cdc25C requires phosphorylation of both S216 and S263 to bind 14-3-3.
Mol Cell Proteomics. 2010 Dec 28. [Epub ahead of print]

Ang SF, Zhao ZS, Lim L and Manser E (2010)
DAAM1 is a formin required for centrosome re-orientation during cell migration.
PLoS One. 2010 Sep 29;5(9). pii: e13064.

Zhao ZS and Manser E (2010)
Do PAKs make good drug targets?
F1000 Biology Report Published 23 Sep 2010

Ng YW, Raghunathan D, Chan PM, Baskaran Y, Smith DJ, Lee CH, Verma C, Manser E.
Why an A-loop phospho-mimetic fails to activate PAK1: understanding an inaccessible kinase state by molecular dynamics simulations.


Department: Edward MANSER


Designation: ARAP Student

Email: raftinuo@student.imcb.a-star.edu.sg

Name: Zhuoshen ZHAO

Designation: Research Assistant Professor

Email: zszhao@imcb.a-star.edu.sg

Name: Yohendran BASKARAN

Designation: Research Fellow

Email: yohendranb@imcb.a-star.edu.sg

Name: Elsa Yuen Wai NG

Designation: Senior Research Officer

Email: ywng@imcb.a-star.edu.sg

Name: Naila Omar Khayyam ALIEVA

Designation: Research Scientist

Email: alievanok@imcb.a-star.edu.sg