Lih Feng CHEOW

The Microfluidics Systems Biology lab

Research

The Microfluidics Systems Biology lab

The Microfluidics Systems Biology lab was established to address challenging questions related to cancer and developmental biology, infectious disease, and other areas of cell and molecular biology using cutting-edge microfluidic devices and novel applications of high-throughput DNA sequencing. The lab is focused on several project areas, which include:

1) genotyping single cells isolated from genetically heterogeneous populations such as tumors and microbial consortia
2) characterizing transcriptional and epigenetic regulation of gene expression by automated chromatin immunoprecipitation
3) mapping regulatory networks and macromolecular assemblies using high-throughput measurements of protein-protein and protein-DNA binding affinities

Our lab collaborates closely with investigators from other A*STAR research institutes, universities, and industry.

Research in the MSB lab is directed byProf. Stephen Quakeunder an A*STAR Visting Investigatorship Programme grant, and by the lab's co-principal investigators,Dr. William F. Burkholder(IMCB) andProf. Yin Thai Chan(IMRE and NUS).

All enquiries may be directed toDr. William BurkholderandDr. Yin ThaiChan.




The Microfluidics Systems Biology lab

The Microfluidics Systems Biology lab was established to address challenging questions related to cancer and developmental biology, infectious disease, and other areas of cell and molecular biology using cutting-edge microfluidic devices and novel applications of high-throughput DNA sequencing. The lab is focused on several project areas, which include:

1) genotyping single cells isolated from genetically heterogeneous populations such as tumors and microbial consortia
2) characterizing transcriptional and epigenetic regulation of gene expression by automated chromatin immunoprecipitation 
3) mapping regulatory networks and macromolecular assemblies using high-throughput measurements of protein-protein and protein-DNA binding affinities

Our lab collaborates closely with investigators from other A*STAR research institutes, universities, and industry. 

Research in the MSB lab is directed by Prof. Stephen Quake under an A*STAR Visting Investigatorship Programme grant, and by the lab's co-principal investigators, Dr. William F. Burkholder (IMCB) and Prof. Yin Thai Chan (IMRE and NUS).

All enquiries may be directed to Dr. William Burkholder and Dr. Yin Thai Chan.

 

 
The Microfluidics Systems Biology lab

The Microfluidics Systems Biology lab was established to address challenging questions related to cancer and developmental biology, infectious disease, and other areas of cell and molecular biology using cutting-edge microfluidic devices and novel applications of high-throughput DNA sequencing. The lab is focused on several project areas, which include:

1) genotyping single cells isolated from genetically heterogeneous populations such as tumors and microbial consortia
2) characterizing transcriptional and epigenetic regulation of gene expression by automated chromatin immunoprecipitation 
3) mapping regulatory networks and macromolecular assemblies using high-throughput measurements of protein-protein and protein-DNA binding affinities

Our lab collaborates closely with investigators from other A*STAR research institutes, universities, and industry. 

Research in the MSB lab is directed by Prof. Stephen Quake under an A*STAR Visting Investigatorship Programme grant, and by the lab's co-principal investigators, Dr. William F. Burkholder (IMCB) and Prof. Yin Thai Chan (IMRE and NUS).

All enquiries may be directed to Dr. William Burkholder and Dr. Yin Thai Chan.

 

 
     
     
 
     
     
 

Staff

Department: Lih Feng CHEOW

Name: William BURKHOLDER

Designation: Visiting Principal Investigator

Email: wburkholder@imcb.a-star.edu.sg


Name: Yin Thai CHAN

Designation: Principal Investigator

Email: chanyt@imre.a-star.edu.sg


Name: Stephen QUAKE

Designation: Visting Investigator

Email: quake@stanford.edu


Name: Casslynn Wei Qian KOH

Designation: Senior Research Officer

Email: wqkoh@imcb.a-star.edu.sg


Name: Yongqiang LUO

Designation: PhD Student

Email: luoyq@imcb.a-star.edu.sg


Name: Sho Wee Siong GOH

Designation: Research Fellow

Email: wsgoh@imcb.a-star.edu.sg


Name: Yeek Teck GOH

Designation: Research Officer

Email: ytgoh@imcb.a-star.edu.sg


Publications

The following research articles from the Quake lab describe some of the applications of microfluidics to biomedical research that our lab is optimizing and making available to other investigators through research collaborations.

Automated chromatin immunoprecipitation:

Wu, A.R., Hiatt, J.B., Lu, R., Attema, J.L., Lobo, N.A., Weissman, I.L., Clarke, M.F., and Quake, S.R. (2009). Automated microfluidic chromatin immunoprecipitation from 2,000 cells.
Lab Chip 9, 1365-1370.

Single-cell genotyping:

Fan, H.C., Wang, J., Potanina, A., and Quake, S.R. (2011).
Whole-genome molecular haplotyping of single cells.
Nat Biotechnol 29, 51-57.

Blainey, P.C., Mosier, A.C., Potanina, A., Francis, C.A., and Quake, S.R. (2011).
Genome of a low-salinity ammonia-oxidizing archaeon determined by single-cell and metagenomic analysis.
PLoS One 6, e16626.

Marcy, Y., Ouverney, C., Bik, E.M., Losekann, T., Ivanova, N., Martin, H.G., Szeto, E., Platt, D., Hugenholtz, P., Relman, D.A., et al. (2007).
Dissecting biological "dark matter" with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth.
Proc Natl Acad Sci U S A 104, 11889-11894.

High-throughput quantitative assays of protein-nucleic acid and protein-protein interactions:

Transcription factors

Maerkl, S.J., and Quake, S.R. (2007).
A systems approach to measuring the binding energy landscapes of transcription factors.
Science 315, 233-237.

Maerkl, S.J., and Quake, S.R. (2009). Experimental determination of the evolvability of a transcription factor.
Proc Natl Acad Sci U S A 106, 18650-18655.

Fordyce, P.M., Gerber, D., Tran, D., Zheng, J., Li, H., DeRisi, J.L., and Quake, S.R. (2010).
De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis.
Nat Biotechnol 28, 970-975.

RNA binding proteins / small molecule screens

Einav, S., Gerber, D., Bryson, P.D., Sklan, E.H., Elazar, M., Maerkl, S.J., Glenn, J.S., and Quake, S.R. (2008). Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis.
Nat Biotechnol 26, 1019-1027.

Protein-protein interactions

Gerber, D., Maerkl, S.J., and Quake, S.R. (2009).
An in vitro microfluidic approach to generating protein-interaction networks.
Nat Methods 6, 71-74.

Bates, S.R., and Quake, S.R. (2009).
Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device.
Appl Phys Lett 95, 73705.