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Technology Development - p53 Lab

We are primarily interested in technology development with a view to generation of biologics (peptides and miniproteins) targeting dysfunctional p53 pathway components. In particular, we develop and employ a range of directed evolution methodologies for protein engineering and selection. These approaches are also being used to engineer/re-purpose enzymes for industrially relevant applications, with current focus on development of “greener” enzyme alternatives for pharmaceutical synthesis. A further area of interest is development of novel biosensing platforms, with emphasis on modular protein biosensors for facile drug screening and diagnostic applications.

Current members

Farid J. Ghadessy - Group Leader 

Siau Jia Wei 

Chee Min Qi Sharon 

Barindra Sana 

Lau Sze Yi 

Lim Ting Xiang 

Ding Ke 

Recent publications

  1. Development and structural characterization of an engineered multi-copper oxidase reporter of protein-protein interactions. Sana B, Chee SMQ, Wongsantichon J, Raghavan S, Robinson RC, Ghadessy FJ. J Biol Chem. 2019 Feb 15.

  2. Synthetic 10FN3-based mono- and bivalent inhibitors of MDM2/X function.Lau SY, Siau JW, Sobota RM, Wang CI, Zhong P, Lane DP, Ghadessy FJ. Protein Eng Des Sel. 2018 Jul 1;31(7-8):301-312.

  3. Structure-activity studies of Mdm2/Mdm4-binding stapled peptides comprising non-natural amino acids. Chee SMQ, Wongsantichon J, Siau J, Thean D, Ferrer F, Robinson RC, Lane DP, Brown CJ, Ghadessy FJ. PLoS One. 2017 Dec 11;12(12).

  4. Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme libraries. Sana B, Chia KHB, Raghavan SS, Ramalingam B, Nagarajan N, Seayad J, Ghadessy FJ. Biotechnol Biofuels. 2017 Feb 3;10:32.