p53Banner

P53 LABORATORY

 

Welcome to the p53Lab

 
ImageThe p53 lab is focusing on the development of new therapies, new diagnostics and new discoveries in the p53 pathway. 11 Million people living with cancer have specific mutations in the p53 gene leading to the production of a faulty p53 protein. This is half the cases of human cancer. In those that have an apparently normal p53 gene we believe that there are faults in the p53 pathway. We are using a variety of approaches to translate this extraordinary finding into clinical benefit. We work closely with many other groups both in Singapore and in the rest of the world to achieve this. We are using biochemical, biophysical, cell biological and genetic approaches in our work and offer an outstanding international training environment

News

2019

2020


Upcoming Conferences

2020

  • Nov - 7th Antibody Symposium, Singapore (details to follow)

2021


Current Publications


Gault J, Liko I, Landreh M, Shutin D, Bolla JR, Jefferies D, Agasid M, Yen HY, Ladds MJGW, Lane DP, Khalid S, Mullen C, Remes PM, Huguet R, McAlister G, Goodwin M, Viner R, Syka JEP, Robinson CV (2020). Combining Native and 'Omics' Mass Spectrometry to Identify Endogenous Ligands Bound to Membrane Proteins. Nat Methods. 2020 May;17(5):505-508. doi: 10.1038/s41592-020-0821-0.

Kong LR, Ong RW, Tan TZ, Mohamed Salleh NAB, Thangavelu M, Chan JV, Koh LYJ, Periyasamy G, Lau JA, Le TBU, Wang L, Lee M, Kannan S, Verma CS, Lim CM, Chng WJ, Lane DP, Venkitaraman A, Hung HT, Cheok CF, Goh BC (2020).
Targeting Codon 158 p53-mutant Cancers via the Induction of p53 Acetylation. Nat Commun. 2020 Apr 29;11(1):2086. doi: 10.1038/s41467-020-15608-y.

Sedimbi SK, Hägglöf T, Garimella MG, Wang S, Duhlin A, Coelho A, Ingelshed K, Mondoc E, Malin SG, Holmdahl R, Lane DP, Leadbetter EA, Karlsson MCI (2020). Combined Proinflammatory Cytokine and Cognate Activation of Invariant Natural Killer T Cells Enhances anti-DNA Antibody Responses. Proc Natl Acad Sci U S A. 2020 Apr 21;117(16):9054-9063. doi: 10.1073/pnas.1920463117.

Lundsten S, Hernández VA, Gedda L, Sarén T, Brown CJ, Lane DP, Edwards K, Nestor M (2020). Tumor-Targeted Delivery of the p53-Activating Peptide VIP116 With PEG-Stabilized Lipodisks. Nanomaterials (Basel). 2020 Apr 19;10(4):783. doi: 10.3390/nano10040783.

Mortensen ACL, Morin E,
Brown CJ, Lane DP, Nestor M (2020). Enhancing the Therapeutic Effects of in Vitro Targeted Radionuclide Therapy of 3D Multicellular Tumor Spheroids Using the Novel Stapled MDM2/X-p53 Antagonist PM2. EJNMMI Res. 2020 Apr 16;10(1):38. doi: 10.1186/s13550-020-0613-7.


Lim S, Khoo R, Peh KM, Teo J, Chang SC, Ng S, Beilhartz GL, Melnyk RA, Johannes CW, Brown CJ, Lane DP, Henry B, Partridge AW (2020). bioPROTACs as Versatile Modulators of Intracellular Therapeutic Targets Including Proliferating Cell Nuclear Antigen (PCNA). Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):5791-5800. doi: 10.1073/pnas.1920251117.

Bolla JR, Corey RA, Sahin C, Gault J, Hummer A, Hopper JTS, Lane DP, Drew D, Allison TM, Stansfeld PJ, Robinson CV, Landreh M (2020).
A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins. Angew Chem Int Ed Engl. 2020 Feb 24;59(9):3523-3528. doi: 10.1002/anie.201914411.

Ling WL, Ng YL, Wipat A, Lane DP, Gan SK (2020).
The Quantification of Antibody Elements and Receptors Subunit Expression Using qPCR: The Design of VH, VL, CH, CL, FcR Subunits Primers for a More Holistic View of the Immune System. J Immunol Methods. 2020 Jan;476:112683. doi: 10.1016/j.jim.2019.112683.

Ferrer-Gago FJ, Koh LQ, Lane DP (2020). Functionalized Resins for the Synthesis of Peptide Alcohols. Chemistry. 2020 Jan 7;26(2):379-383. doi: 10.1002/chem.201903965.

Diller DJ, Swanson J, Bayden AS, Brown CJ, Thean D, Lane DP, Partridge AW, Sawyer TK, Audie J (2019). Rigorous Computational and Experimental Investigations on MDM2/MDMX-Targeted Linear and Macrocyclic Peptides. Molecules. Dec 14; 24(24). doi: 10.3390/molecules24244586  

Gago FJF, Koh LQ, Lane DP (2019). New Functionalized Resins for the Synthesis of Peptide Alcohols. 14 Oct. Chemistry - a European Journal.

Frosi Y, Inoue K, Ramlan SR, Lane DP, Watanabe T and Brown CJ (2019). Simultaneous measurement of p53:Mdm2 and p53:Mdm4 protein-protein interactions in whole cells using fluorescence labelled foci. Scientific Reports volume 9, Article number: 17933

Pradhan MR, Siau JW, Kannan S, Nguyen MN, Ouaray Z, Kwoh CK, Lane DP, Ghadessy F, Verma CS (2019). Simulations of mutant p53 DNA binding domains reveal a novel druggable pocket. Nucleic Acids Research, 47(4), 1637–1652.

Xue Y, Barker N, Hoon S, He P, Thakur T, Abdeen SR, Maruthappan P, Ghadessy FJ, Lane DP (2019). Bortezomib Stabilizes and Activates p53 in Proliferative Compartments of both Normal and Tumor Tissues In Vivo. Cancer Res, 79(14), 3595-3607.

Partridge AW, Kaan HYK, Juang YC, Sadruddin A, Lim S, Brown CJ, Ng S, Thean D, Ferrer F, Johannes C, Yuen TY, Kannan S, Aronica P, Tan YS, Pradhan MR, Verma CS, Hochman J, Chen S, Wan H, Ha S, Sherborne B2, Lane DP, Sawyer TK (2019). Incorporation of putative helix-breaking amino acids in the design of novel stapled peptides: Exploring biophysical and cellular permeability properties. Molecules, 24(12).

Koh XY, Koh XH, Hwang LA, Ferrer FJ, Rahmat SAB, Lama D, Lane DP (2019). Therapeutic anti-cancer activity of antibodies targeting sulfhydryl bond constrained epitopes on unglycosylated RON receptor tyrosine kinase . Oncogene. doi: 10.1038/s41388-019-0946-8. [Epub ahead of print].

Sana B, Chee SMQ, Wongsantichon J, Raghavan S, Robinson RC, Ghadessy FJ (2019). Development and structural characterization of an engineered multi-copper oxidase reporter of protein-protein interactions. J Biol Chem, 294(17), 7002-7012. doi: 10.1074/jbc.RA118.007141. Epub 2019 Feb 15.

Yuen TY, Brown CJ, Xue Y, Tan YS, Ferrer Gago FJ, Lee XE, Neo JY, Thean D, Kaan HYK, Partridge AW, Verma CS, Lane DP, Johannes CW (2019). Stereoisomerism of stapled peptide inhibitors of the p53-Mdm2 interaction: an assessment of synthetic strategies and activity profiles. Chem Sci, 10(26).

Lama D, Liberatore AM, Frosi Y, Nakhle J, Tsomaia N, Bashir T, Lane DP, Brown CJ, Verma CS, Auvin, S. (2019). Structural insights reveal arecognition feature for tailoring hydrocarbon stapled-peptides againstthe eukaryotic translation initiation factor 4E protein. S. Chem Sci., 10(8), 2489-2500.

Lundgren Mortensen AC, Spiegelberg D, Brown CJ, Lane DP, Nestor M (2019). The Stapled Peptide PM2 Stabilizes p53 Levels and Radiosensitizes Wild-Type p53 Cancer Cells. Front. Oncol., 19 September 2019. doi.org: 10.3389/fonc.2019.0092