Stem cell biology and the developmental origins of health and disease:
Fetal exposures to an adverse intrauterine environment can increase the susceptibility to develop metabolic diseases in adult life. We have established primary Wharton’s jelly derived Mesenchymal Stem Cell (MSC) lines isolated from umbilical cords of Small for Gestational Age (SGA) neonates, as well as babies with maternal background of gestational diabetes (GDM). Our group has shown that MSCs preserve biological features of growth restricted humans and may therefore aid in the identification of molecular pathways and biomarkers for aberrant developmental programming in humans.
Capabilities and projects:
- Established more than 100 primary MSC and HUVEC cell lines with SGA and GDM background, as well as matched controls from the three major ethnic groups in Singapore.
- Lineage specific differentiation of MSCs
- Role of epigenetics (histone modifications and DNA-methylation) in developmental programming
- Next Generation Sequencing techniques such as ChIP-seq., RNA-seq., microRNA-seq.