Lai Guan NG

Principal Investigator

Adj. Ast/Prof NTU, NUS; KKH (Joint Scientist); Adj. Assoc/Prof University of Sydney Medical School


  • CHONG Shu Zhen, Ph.D
  • Maximilien EVRARD, Ph.D
  • TAN Yingrou, Ph.D
  • Jasmine LAU, Ph.D
  • GOH Chi Ching Visiting Scientist
  • Leonard TAN
  • LIONG Ka Hang
  • KWOK, Immaunel Ph.D Student
  • TEH Ye Chean Ph.D Student

Profile

Research Focus

The immune system consists of an extensive network of immune cells distributed among various body compartments, including lymphoid and non-lymphoid organs. A major characteristic of leukocytes is that they rely on their migratory behavior to execute their effector functions in a temporal and anatomical defined manner, and to return to a state of homeostasis after the response. To attain a comprehensive understanding of the cellular and molecular mechanisms of leukocyte function, ideally, the trafficking/migratory activities of leukocytes should be investigated within their native microenvironment in a dynamic manner. The primary goal of our laboratory is to understand how the local micro-milieu impacts on the cellular activities and function of myeloid cells. Specially, we aim to investigate the behavior of neutrophils and monocytes in a compartmentalized manner, i.e. by deciphering their interactive behavior in the bone marrow (BM), within the circulatory system and peripheral tissue/organ. To achieve this, we will make use of a combination of intravital imaging techniques, conventional cellular and molecular approaches, as well as in vivo models. 

Intravital Imaging Models established in the lab

Biography

Dr. Ng completed his Bachelor of Medical Science (Honours) degree from University of New South Wales, Australia in 2000. He was then awarded an International Postgraduate Research Scholarship to conduct his PhD work at the Garvan Institute of Medical Research in Sydney, Australia. After completing his PhD in 2004, he joined Institute of Molecular and Cell Biology in Singapore for his postdoctoral training under the supervision of Professor Lam Kong Peng. In 2006, Dr. Ng joined Professor Wolfgang Weninger’s laboratory at the Wistar Institute in Philadelphia USA. Following his postdoctoral training, Dr. Ng joined Singapore Immunology Network (SIgN) to establish his own laboratory in 2009. He is also an adjunct Assistant Professor at the National University of Singapore and Nanyang Technological University in Singapore. Dr. Ng has also been appointed as a adjunct Scientist at KK Women’s and Children’s Hospital (KKH), Singapore. The primary research focus of his group is to study how immune cells exert their function in the context of intact organs by intravital multiphoton microscopy. Using a combination of functional and in vivo imaging studies, his group aims to determine the sequence of cellular and molecular events involved in the regulation of immune cell homeostasis. Dr. Ng’s laboratory has established several intravital imaging approaches for direct visualization of immune cell behaviour in the skin, bone marrow, lung, brain and muscle. Dr. Ng’s contributions to the field of immunology research is best exemplified by his multiple publications in leading journals such as Journal of Experimental Medicine, Advanced Materials, Nature Protocols and Journal of Investigative Dermatology. He is also a review editor of Frontiers in Immunotherapies and Vaccines and an academic editor for PloS One. Dr. Ng also recently joined the editorial board of Cellular Immunology and Journal of Dermatological Research.

Projects

  • To determine the behavior of cutaneous innate cells in response to sterile injury

    The skin represents the interface between the external environment and internal tissues. Tissue damage in the skin, whether triggered by infection, ischaemia or trauma, induces the coordinated recruitment of leukocytes. While these recruited cells participate in tissue repair by clearing cell debris, they may potentially cause unwanted tissue damage. Because of these dual functions, it is imperative to better understand the regulatory cues involved in their attraction and accumulation to sites of tissue injury.

    read more
  • To determine how circulating neutrophil counts are regulated

    Neutrophils are key mediators of host defense against microbial agents. The distribution of neutrophils between different tissue compartments is a tightly regulated process to maintain stable blood neutrophil counts at resting state, to allow rapid mobilization of neutrophils in response to inflammatory stimuli, and to return to a state of homeostasis after the immune response. The dynamic regulation of circulating neutrophil numbers depends on a complex and dynamic interplay between various cellular and molecular components in multiple different tissue compartments, and is thus difficult to dissect using conventional laboratory techniques. To overcome this challenge, we studied neutrophil mobilization using a dynamic and integrative physiological approach.

    read more
  • Understand the role of CXCR4 in monocyte homeostasis and inflammatory diseases

    Monocytes are key players in the initiation of immune responses and maintenance of tissue integrity. Despite rapid progress in the field of monocyte research, the mechanisms that regulate their homeostasis and trafficking patterns among tissue compartments remain poorly defined. Like all leukocytes, monocytes rely heavily on their migratory behavior to execute their effector functions in a temporal and anatomical defined. Conceivably, dysregulations in their trafficking patterns have thus been implicated in multiple diseases that include cancer, neurological, metabolic and cardiovascular disorders. Here, we aim to study how monocyte compartmentalization influences their function.

    read more

Publications

2016

Goh CC, Li JL, Becker D, Weninger W, Angeli V, Ng LG. Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses. J Vis Exp. 2016 Dec 22;(118).

Fateminia SM, Wang Z, Goh CC, Manghnani PN, Wu W, Mao D, Ng LG, Zhao Z, Tang BZ, Liu B. Nanocrystallization: A Unique Approach to Yield Bright Organic Nanocrystals for Biological Applications. Adv Mater. 2016 Nov 2. [Epub ahead of print]

Chong SZ, Evrard M, Devi S, Chen J, Lim JY, See P, Zhang Y, Adrover JM, Lee B, Tan L, Li JL, Liong KH, Phua C, Balachander A, Boey A, Liebl D, Tan SM, Chan JK, Balabanian K, Harris JE, Bianchini M, Weber C, Duchene J, Lum J, Poidinger M, Chen Q, Rénia L, Wang CI, Larbi A, Randolph GJ, Weninger W, Looney MR, Krummel MF, Biswas SK, Ginhoux F, Hidalgo A, Bachelerie F, Ng LG. CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses. J Exp Med. 2016 Oct 17;213(11):2293-2314.

Lau J, Goh CC, Devi S, Keeble J, See P, Ginhoux F, Ng LG. Intravital multiphoton imaging of mouse tibialis anterior muscle. IntraVital. 2016 Apr 8; 5:2, e1156272

Ariyasu S, Too PC, Mu J, Goh CC, Ding Y, Tnay YL, Yeow EK, Yang L, Ng LG, Chiba S, Xing B. Glycopeptide antibiotic analogs for selective inactivation and two-photon imaging of vancomycin-resistant strains. Chem Commun (Camb). 2016 Mar 17;52(25):4667-70.

Chen B, Feng G, He B, Goh C, Xu S, Ramos-Ortiz G, Aparicio-Ixta L, Zhou J, Ng L, Zhao Z, Liu B, Tang BZ. Silole-Based Red Fluorescent Organic Dots for Bright Two-Photon Fluorescence In vitro Cell and In vivo Blood Vessel Imaging. Small. 2016 Feb;12(6):782-92.

Li JL, Lim CH, Tay FW, Goh CC, Devi S, Malleret B, Lee B, Bakocevic N, Chong SZ, Evrard M, Tanizaki H, Lim HY, Russell B, Renia L, Zolezzi F, Poidinger M, Angeli V, John AL, Harris JE, Tey HL, Tan SM, Kabashima K, Weninger W, Larbi A, Ng LG. Neutrophils Self-Regulate Immune Complex-Mediated Cutaneous Inflammation Through CXCL2. J Invest Dermatol. 2016 Feb;136(2):416-24.

2015

Geng J, Goh CC, Qin W, Liu R, Tomczak N, Ng LG, Tang BZ, Liu B. Silica shelled and block copolymer encapsulated red-emissive AIE nanoparticles with 50% quantum yield for two-photon excited vascular imaging. Chem Commun (Camb). 2015 Sep 7;51(69):13416-9. 

Almeida FF, Tenno M, Brzostek J, Li JL, Allies G, Hoeffel G, See P, Ng LG, Fehling HJ, Gascoigne NR, Taniuchi I, Ginhoux F. Identification of a novel lymphoid population in the murine epidermis. Sci Rep. 2015 Jul 30;5:12554. 

Xiang J, Cai X, Lou X, Feng G, Min X, Luo W, He B, Goh CC, Ng LG, Zhou J, Zhao Z, Liu B, Tang BZ. Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization. ACS Appl Mater Interfaces. 2015 Jul 15;7(27):14965-74.

Gunawan M, Venkatesan N, Loh JT, Wong JF, Berger H, Neo WH, Li LY, Win MK, Yau YH, Guo T, See PC, Yamazaki S, Chin KC, Gingras AR, Shochat SG, Ng LG, Sze SK, Ginhoux F, Su IH. The methyltransferase Ezh2 controls cell adhesion and migration through direct methylation of the extranuclear regulatory protein talin. Nat Immunol. 2015 May;16(5):505-16.

Goh CC, Li JL, Devi S, Bakocevic N, See P, Larbi A, Weninger W, Ginhoux F, Angeli V, Ng LG. Real-time Imaging of Dendritic Cell Responses To Sterile Tissue Injury. J Invest Dermatol. 2015 Apr;135(4):1181-4.

Hoeffel G, Chen J, Lavin Y, Low D, Almeida FF, See P, Beaudin AE, Lum J, Low I, Forsberg EC, Poidinger M, Zolezzi F, Larbi A, Ng LG, Chan JK, Greter M, Becher B, Samokhvalov IM, Merad M, Ginhoux F. C-myb(+) erythro-myeloid progenitor-derived fetal monocytes give rise to adult tissue-resident macrophages. Immunity. 2015 Apr 21;42(4):665-78. 

Evrard M, Chong SZ, Devi S, Chew WK, Lee B, Poidinger M, Ginhoux F, Tan SM, Ng LG. Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy. J Leukoc Biol. 2015 Mar;97(3):611-9.

Tan KW, Evrard M, Tham M, Hong M, Huang C, Kato M, Prevost-Blondel A, Donnadieu E, Ng LG, Abastado JP. Tumor stroma and chemokines control T-cell migration into melanoma following Temozolomide treatment. Oncoimmunology. 2015 Feb 25;4(2):e978709 

Malleret B, Li A, Zhang R, Tan KS, Suwanarusk R, Claser C, Cho JS, Koh EG, Chu CS, Pukrittayakamee S, Ng ML, Ginhoux F, Ng LG, Lim CT, Nosten F, Snounou G8, Rénia L, Russell B. Plasmodium vivax: restricted tropism and rapid remodelling of CD71 positive reticulocytes. Blood. 2015 Feb 19;125(8):1314-24.

2014

Mu J, Liu F, Rajab MS, Shi M, Li S, Goh C, Lu L, Xu QH, Liu B, Ng LG, Xing B. A Small-Molecule FRET Reporter for the Real-Time Visualization of Cell-Surface Proteolytic Enzyme Functions. Angew Chem Int Ed Engl. 2014 Dec 22;53(52):14357-62.  

Braniste V, Al-Asmakh M, Kowal C, Anuar F, Abbaspour A, Tóth M, Korecka A, Bakocevic N, Guan NL, Kundu P, Gulyás B, Halldin C, Hultenby K, Nilsson H, Hebert H, Volpe BT, Diamond B, Pettersson S. The gut microbiota influences blood-brain barrier permeability in mice. Sci Transl Med. 2014 Nov 19;6(263):263ra158. 

Natsuaki Y, Egawa G, Nakamizo S, Ono S, Hanakawa S, Okada T, Kusuba N, Otsuka A, Kitoh A, Honda T, Nakajima S, Tsuchiya S, Sugimoto Y, Ishii KJ, Tsutsui H, Yagita H, Iwakura Y, Kubo M, Ng LG, Hashimoto T, Fuentes J, Guttman-Yassky E, Miyachi Y, Kabashima K. Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin. Nat Immunol. 2014 Nov;15(11):1064-9.

Geng J, Goh CC, Tomczak N, Liu J, Liu R, Ma L, Ng LG, Gurzadyan GG, Liu B. Micelle/Silica Co-protected Conjugated Polymer Nanoparticles for Two-Photon Excited Brain Vascular Imaging. Chem Mater. 2014, 26 (5), pp 1874–1880 

Bakocevic N, Claser C, Yoshikawa S, Jones LA, Chew S, Goh CC, Malleret B, Larbi A, Ginhoux F, de Lafaille MC, Karasuyama H, Renia L, Ng LG. CD41 is a reliable identification and activation marker for murine basophils in the steady-state and during helminth and malarial infections. Eur J Immunol. 2014 Jun;44(6):1823-34.

Wang XN, McGovern N, Gunawan M, Richardson C, Windebank M, Siah TW, Lim HY, Fink K, Li JL, Ng LG, Ginhoux F, Angeli V, Collin M, Haniffa M. A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels. J Invest Dermatol. 2014 Apr;134(4):965-74.

Chong SZ, Tan KW, Wong FH, Chua YL, Tang Y, Ng LG, Angeli V, Kemeny DM. CD8 T-cells regulate allergic contact dermatitis by modulating CCR2-dependent TNF/iNOS-expressing Ly6C+CD11b+ monocytic cells. J Invest Dermatol. 2014 Mar;134(3):666-76.

Claser C, Malleret B, Peng K, Bakocevic N, Gun SY, Russell B, Ng LG, Rénia L. Rodent Plasmodium-infected red blood cells: Imaging their fates and interactions within their hosts. Parasitol Int. 2014 Feb;63(1):187-94.

Abtin A, Jain R, Mitchell AJ, Roediger B, Brzoska AJ, Tikoo S, Cheng Q, Ng LG, Cavanagh LL, von Andrian UH, Hickey MJ, Firth N, Weninger W. Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection. Nat Immunol. 2014 Jan;15(1):45-53.

2013

Ding D , Goh CC , Feng G , Zhao Z, Liu J, Liu R, Tomczak N, Geng J, Tang BZ, Ng LG, Liu B. Ultrabright organic dots with aggregation-induced emission characteristics for real-time two-photon intravital vasculature imaging. Adv Mater. 2013 Nov 13;25(42):6083-8.

Tan KW, Chong SZ, Wong FH, Evrard M, Tan SM, Keeble J, Kemeny DM, Ng LG, Abastado JP, Angeli V. Neutrophils contribute to inflammatory lymphangiogenesis by increasing VEGF-A bioavailability and secreting VEGF-D. Blood. 2013 Nov 21;122(22):3666-77.

Zhao Z, Chen B, Geng J, Chang Z, Aparicio-Ixta L, Nie H, Goh CC, Ng LG, Qin A, Ramos-Ortiz G, Liu B, Tang BZ. Red Emissive Biocompatible Nanoparticles from Tetraphenylethene-Decorated BODIPY Luminogens for Two-Photon Excited Fluorescence Cellular Imaging and Mouse Brain Blood Vascular Visualization. Part Part Syst Charact. Oct 21 2013. [Epub ahead of print]

Devi S, Wang Y, Chew WK, Lima R, A-González N, Mattar CN, Chong SZ, Schlitzer A, Bakocevic N, Chew S, Keeble JL, Goh CC, Li JL, Evrard M, Malleret B, Larbi A, Renia L, Haniffa M, Tan SM, Chan JK, Balabanian K, Nagasawa T, Bachelerie F, Hidalgo A, Ginhoux F, Kubes P, Ng LG. Neutrophil mobilization via plerixafor-mediated CXCR4 inhibition arises from lung demargination and blockade of neutrophil homing to the bone marrow. J Exp Med. 2013 Oct 21;210(11):2321-36.

Tong PL, Qin J, Cooper CL, Lowe PM, Murrell DF, Kossard S, Ng LG, Roediger B, Weninger W, Haass NK. A quantitative approach to histopathological dissection of elastin-related disorders using multiphoton microscopy. Br J Dermatol. 2013 Oct;169(4):869-879.

Chong SZ, Evrard M, Ng LG. Lights, camera and action: vertebrate skin sets the stage for immune cell interaction with arthropod-vectored pathogens. Front. Immunol. 2013; 4:286.

SchlitzerA, McGovern N, Teo P, Zelante T, Atarashi K, Low D, Ho AW, See P, Shin A, Wasan PS, Hoeffel G,  Malleret B, Heiseke A, Chew S, Jardine L, Purvis HA, Hilkens CM, John Tam J, Poidinger M, Stanley RE, Krug AB, Renia L, Sivasankar B, Ng LG, Collin M, Ricciardi-Castagnoli P, Honda K, Haniffa M, Ginhoux F. IRF4 transcription factor-dependent CD11b+ dendritic cells in human and mouse control mucosal IL-17 cytokine responses. Immunity. 2013 May 23;38(5):970-983.

Wai LE, Narang V, Gouaillard A, Ng LG, Abastado JP. In silico modeling of cancer cell dissemination and metastasis. Ann N Y Acad Sci. 2013 May; 1284(1):71-74.

2012

Li JL, Ng LG. Peeking into the secret life of neutrophils. Immunol Res. 2012 Sep;53(1-3):168-81. 

Hoeffel G, Wang Y, Greter M, See P, Teo P, Malleret B, Leboeuf M, Low D, Oller G, Almeida F, Choy SH, Grisotto M, Renia L, Conway SJ, Stanley ER, Chan JK, Ng LG, Samokhvalov IM, Merad M, Ginhoux F. Adult Langerhans cells derive predominantly from embryonic fetal liver monocytes with a minor contribution of yolk sac–derived macrophages. J Exp Med. 2012 Jun 4;209(6):1167-81.

Li JL, Goh CC, Keeble JL, Qin JS, Roediger B, Jain R, Wang Y, Chew WK, Weninger W, Ng LG. Intravital multiphoton imaging of immune responses in the mouse ear skin. Nat Protoc. 2012 Jan 12;7(2):221-34.

2011

Shklovskaya E, O'Sullivan BJ, Ng LG, Roediger B, Thomas R, Weninger W, Fazekas de St Groth B. Langerhans cells are precommitted to immune tolerance induction. Proc Natl Acad Sci U S A. 2011 Oct 17. [Epub ahead of print]

Ng LG, Qin JS, Roediger B, Wang Y, Jain R, Cavanagh LL, Smith AL, Jones CA, de Veer M, Grimbaldeston MA, Meeusen EN, Weninger W. Visualizing the Neutrophil Response to Sterile Tissue Injury in Mouse Dermis Reveals a Three-Phase Cascade of Events. J Invest Dermatol. Oct 2011; 131(10):2058-2068.

Tal O, Lim HY, Gurevich I, Milo I, Shipony Z, Ng LG, Angeli V, Shakhar G. DC mobilization from the skin requires docking to immobilized CCL21 on lymphatic endothelium and intralymphatic crawling. J Exp Med. Sep 26 2011; 208(10):2141-2153. 

Sumaria N, Roediger B, Ng LG, Qin J, Pinto R, Cavanagh LL, Shklovskaya E, Fazekas de St Groth B, Triccas JA, Weninger W. Cutaneous immunosurveillance by self-renewing dermal {gamma}{delta} T cells. J Exp Med. 2011 Mar 14;208(3):505-18.

Kong CU, Ng LG, Nambiar JK, Spratt JM, Weninger W, Triccas JA. Targeted induction of antigen expression within dendritic cells modulates antigen-specific immunity afforded by recombinant BCG. Vaccine. 2011 Feb 4;29(7):1374-81.

2010

Ginhoux F, Ng LG, Merad M. Understanding the Murine Cutaneous Dendritic Cell Network to Improve Intradermal Vaccination Strategies. Curr Top Microbiol Immunol. Nov 6 2010. [E-pub ahead of print]

Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, Mehler MF, Conway SJ, Ng LG, Stanley ER, Samokhvalov IM, Merad M. Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science. Nov 5 2010; 330(6005):841-845.

2009

John B, Harris TH, Tait ED, Wilson EH, Gregg B, Ng LG, Mrass P, Roos DS, Dzierszinski F, Weninger W, and Hunter CA. Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii. PLoS Pathog. 2009 Jul;5(7):e1000505.

Hoeller C, Richardson SK, Ng LG, Valero T, Wysocka M, Rook AH, and Weninger W. In vivo imaging of cutaneous T-cell lymphoma migration to the skin. Cancer Res. 2009 Apr 1;69(7):2704-8.

2008

Mrass P, Kinjyo I, Ng LG, Reiner SL, Pure E, and Weninger W. CD44 mediates successful interstitial navigation by killer T cells and enables efficient antitumor immunity. Immunity. 2008 Dec 19;29(6):971-85.

Roediger B, Ng LG*, Smith AL, Fazekas de St Groth B, and Weninger W. Visualizing dendritic cell migration within the skin. Histochem Cell Biol. 2008 Dec;130(6):1131-46.

Ng LG, Hsu A, Mandell MA, Roediger B, Hoeller C, Mrass P, Iparraguirre A, Cavanagh LL, Triccas JA, Beverley SM, Scott P, and Weninger W. Migratory dermal dendritic cells act as rapid sensors of protozoan parasites. PLoS Pathog. 2008 Nov;4(11):e1000222.

Ng LG*, Mrass P, Kinjyo I, Reiner SL, and Weninger W. Two-photon imaging of effector T-cell behavior: lessons from a tumor model. Immunol Rev. 2008 Feb;221147-62.

2006

Mrass P, Takano H, Ng LG, Daxini S, Lasaro MO, Iparraguirre A, Cavanagh LL, von Andrian UH, Ertl HC, Haydon PG, and Weninger W. Random migration precedes stable target cell interactions of tumor-infiltrating T cells. J Exp Med. 2006 Nov 27;203(12):2749-61.

Fletcher CA, Sutherland AP, Groom JR, Batten ML, Ng LG, Gommerman J, and Mackay F. Development of nephritis but not sialadenitis in autoimmune-prone BAFF transgenic mice lacking marginal zone B cells. Eur J Immunol. 2006 Sep;36(9):2504-14.

Ng LG, Ng CH, Woehl B, Sutherland AP, Huo J, Xu S, Mackay F, and Lam KP. BAFF costimulation of Toll-like receptor-activated B-1 cells. Eur J Immunol. 2006 Jul;36(7):1837-46.

2005

Sutherland AP, Ng LG*, Fletcher CA, Shum B, Newton RA, Grey ST, Rolph MS, Mackay F, and Mackay CR. BAFF augments certain Th1-associated inflammatory responses. J Immunol. 2005 May 1;174(9):5537-44.

Ng LG, Mackay CR, and Mackay F. The BAFF/APRIL system: life beyond B lymphocytes. Mol Immunol. 2005 May;42(7):763-72.

2004

Shulga-Morskaya S, Dobles M, Walsh ME, Ng LG, MacKay F, Rao SP, Kalled SL, and Scott ML. B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation. J Immunol. 2004 Aug 15;173(4):2331-41.

Ng LG*, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, and Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol. 2004 Jul 15;173(2):807-17.

Batten M, Fletcher C, Ng LG, Groom J, Wheway J, Laabi Y, Xin X, Schneider P, Tschopp J, Mackay CR, and Mackay F. TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma. J Immunol. 2004 Jan 15;172(2):812-22.

CV

Education & Professional Training

2014 – Present  Adjunct Scientist – KK Women’s and Children’s Hospital (KKH), Singapore 

2012 – Present  Adjunct Assistant Professor – National University of Singapore, Department of Microbiology, Singapore

2011 – Present  Adjunct Assistant Professor – Nanyang Technological University, School of Biological Sciences, Singapore

2009 – Present  Principal Investigator – Singapore Immunology Network, Singapore

2007 – 2009  Research Fellow – The Centenary Institute, University of Sydney, Australia
Supervisor: Prof. Wolfgang Weninger

2006 – 2007  Postdoctoral Fellow – The Wistar Institute, Philadelphia, Pennsylvania, USA 
Supervisor: Prof. Wolfgang Weninger

2004 – 2006 Research Fellow – Institute of Molecular and Cell Biology, Singapore
Supervised by A/Prof. Kong-Peng Lam

2001 – 2004 PhD Scholar – The Garvan Institute of Medical Research, University of New South Wales, Faculty of Medicine, Australia
Supervised by Prof. Fabienne Mackay and Prof. Charles Mackay

2000 Bachelor of Medical Science Honours (1st Class) – The Garvan Institute of Medical Research, University of New South Wales, School of Physiology and Pharmacology, Australia
Supervised by A/Prof. Kenneth Williams and Dr Garry Corthals

1997-1999  Bachelor of Medical Science – University of Sydney, Australia
Major in Pathology and Pharmacology.

Scholarships

2001-2004 International Postgraduate Research Scholarship – University of New South Wales, Sydney, Australia

Research Grants

1. NHMRC Project Grants: Dynamics and mechanisms of immune complex-mediated skin inflammation. 2016-18 ($AUD526,466.50/3 yrs). CIA: W. Weninger and CIB: L.G. Ng

2. NHG Small Innovative Grant: Genomic Profiling of Type III hypersensitivity in Cutaneous Vasculitis. 2015-17 ($76,000/2 yrs) Clinical PI: H.L. Tay, Non-Clinical PI: L.G. Ng

3. A-STAR-NHG-NTU Skin Research Grant: Pathophysiology of itch in atomic dermatitis and psoriasis (SRG/14019). 2014-17 ($S500,000/3 yrs). Clinical PI: H.L. Tay, Non-Clinical PI: L.G. Ng

4. 12th JCO Grants: Development of cross-platform imaging probes for the study of neutrophil function in vivo (1431AFG108). 2013-14 ($S194,160). Project Director: L.G. Ng, Lead Investigators: B Liu and K Li.

5. A*STAR-MINDEF Grants: The development of a broadly protective influenza vaccine and visualization of immunological changes mediated by an immuno-modulating agent as mechanistic basis for effective medical countermeasures with military applications (10/1/50/19/669). 2011-14 ($S397,790/3 yrs). Lead Investigator: Gary Lau and Co-Investigator: L.G. Ng

6. Cancer Institute NSW Research Innovation Grants: Intravital cell-cycle imaging as a novel approach to design combined targeting strategies of independent signaling pathways in melanoma. 2009 ($AUD50,000). CIA: N. Haass and CIB: L.G. Ng

7. NHMRC Project Grants: Real-time visualisation of dendritic cell responses during cutaneous Leishmania infection. 2009-11 ($AUD491,250/3 yrs). CIA: W. Weninger and CIB: L.G. Ng

8. The Clive And Vera Ramaciotti Foundations Establishment Grants: Visualising the structural changes of the lung during inflammatory responses by multiphoton microscopy. 2009 ($AUD30,000). CIA: L.G. Ng

9. ARC FABL Network Support Grants: Development and optimisation of OPO-based multiphoton fluorescence imaging. 2008 ($AUD10,500). CIA: A.L. Smith and CIB: L.G. Ng

10. The University of Sydney Bridging Support Grants: Real-time visualisation of dendritic cell responses in the skin. 2008 ($AUD50,000). CIA: W. Weninger and CIB: L.G. Ng

Supervision & Mentoring

2014-Present  Immanuel Kwok (NTU-SIgN, PhD student), Chun Hwee Lim (NTU, PhD student)

2009-Present  Maximilien Evrard (NTU-SIgN, PhD student), Chi Ching Goh (NUS, PhD Student)

2009-2015  Jackson Li (NTU, PhD student, graduated)

2015  Tracy Thanh Ngo (University of California attachment student, USA)

2015  Valerie Le Bouteiller (Ecole Normale Suprieure student, France)

2014  Chun Hwee Lim and Jia Wang Chua (NTU, FYP)

2013  Wee LiXuan Armanda (NTU, FYP), Ekaterina Kinnear (Imperial College, PhD Summer Attachment)

2012  Fen Wei Tay, Bi Xia Kow, May Qi Chew (NTU, FYP)

2011 Kajsa Novan (Karolinska Institute, FYP student)

2010-2011  Vanessa Manoharan (NUS, Honours student)

2006-2007  Steve Suway (Summer student, The Wistar Institute, USA)

2005-2006  Huibin Zhang (Attachment student, IMCB, Singapore)

Professional Societies

2008-Present  Singapore Society For Immunology

2002-Present  Australasian Society For Immunology

2007-2010  Fluorescence Applications In Biotechnology & Life Sciences (FABLS) ARC Network

Professional Activities

2015-present  Assistant Treasurer for Singapore Society For Immunology

2014-present  Editorial Board Member for Cellular Immunology

2014-present  Academic Editor for PLOS ONE open access journal

2011-Present  Review Editor of Frontiers in Immunotherapies and Vaccines

2014-2015  Program Board Member for NTU-SIgN Immunology PhD Program

2013-2015  Executive Committee Member for Singapore Society For Immunology

Lab

alt

from left: Jackson LI (Attachment student), CHEW Weng Keong (RO), NG Lai Guan, Teoh Kim Tat (RF), Samantha CHEW (RO), WANG Yilin (RF)

alt

Back to Principle Investigators List