Research

The mission of the TLGM is the application of clinical genetics-based biomedical research to the discovery and development of novel diagnostics and therapeutics.

Research Programmes:

Research efforts within TLGM are currently focused in three areas:

Cardiometabolic Disease Program

 

Neurodegenerative Diseases Program

 

Cardiometabolic diseases are a leading cause of morbidity and mortality in both the developing and developed countries. Led by Dr. Roshni Singaraja, our group is interested in translational research with a focus on identification and  functional characterization of pathways involved in cardiometabolic disease. Our aim is to identify novel genes, validate the novel genes utilising human genetic association studies, and bridge the gap between the human association studies and functional mechanisms utilizing human investigative medicine studies, animal models and in vitro studies relevant to the disease states of diabetes mellitus, atherogenic dyslipidemia and cardiovascular disease.

 

The neurodegenerative diseases (NDD) laboratory, led by Dr. Mahmoud Pouladi, focuses on understanding how discrete genetic factors contribute to the development of NDD. Our aim is to define key disease processes underlying neurodegenerative disorders and to develop novel therapeutic approaches for intervention.

     

Pharmacogenomics & Personalized Medicine Program

 

Integrative Omics Program

 

Led by Dr. Liam Brunham, the PGx laboratory focuses on understanding how genetic information can be used to predict, diagnosis and treat disease. We are particularly focused on identifying genetic variation that predicts response to medications and understanding molecular mechanisms of drug toxicity.

 

Led by Dr Marie Loh, , the Integrative Omics group focuses on the identification of biomarkers and mechanisms underlying cardiometabolic diseases and associated disturbances, with a specific interest in transethnic studies. The group aims to develop new strategies for disease prediction, prevention and treatment through the integrative analysis of high-throughput omics data across different platforms (array- and NGS- based).

     

Clinical Trial Innovation Lab

 

 

Led by Dr.Chester Drum, the clinical trial innovation lab focuses on new ways of scientific engagement with real world patients. Treating patients as partners in discovery, the lab uses cutting edge technology and protocol design to maximize the information from clinical investigations. Of particular interest is how small volume biosampling of nonrandomised, case-control cohorts can aid in more efficient regulatory approval and more rapid clinical acceptance of new diagnostics. Biomarkers are both discovered and validated using a dedicated quantitative LCMS facility, while incorporating novel materials and devices sourced from the broader Singapore research community.


TLGM Publications

2017

Chen MZ, Moily NS, Bridgford J, Wood RJ, Radwan M, Bridgford J, Smith TA, Song Z, Tang BZ, Tilley L, Xu X, Reid GE, Pouladi MA, Hong Y, Hatters DM. A thiol probe for measuring unfolded protein load and proteostasis in cells. Nature Communications. 2017. In Press.

Takata K, Kozaki T, Lee CZW, Thion MS, Otsuka M, Lim S, Utami KH, Fidan K, Park DS, Malleret B, Chakarov S, See P, Low D, Low G, Garcia-Miralles M, Zeng R, Zhang J, et al. Garel S, Pouladi MA, Ginhoux F. Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function. Immunity. 2017 Jul 18;47(1):183-198.e6. doi: 10.1016/j.immuni.2017.06.017.

Xu X, Tay YL, Huang Y, Yoon SI, Sim B, Ooi J, Utami KH, Ziaei A, Ng B, Radulescu C, Ng A, Low D, Loh M, Venkatesh B, Ginhoux F, Augustine GJ, Pouladi MA. Reversal of phenotypic abnormalities by CRISPR/Cas9-mediated gene correction in Huntington disease patient-derived induced pluripotent stem cells. Stem Cell Reports. 2017 Mar 14;8(3):619-633. doi: 10.1016/j.stemcr.2017.01.022.

Julien SG, Kim SY, Brunmeir R, Sinnakannu JR, Ge X, Li H, Ma W, Yaligar J, Prakash B, Velan SS, Röder PV, Zhang Q, Sim CK, Wu J, Garcia-Miralles M, Pouladi MA, Xie W, McFarlane C, Han W, Xu F. Narciclasine attenuates diet-induced obesity by promoting oxidative metabolism in skeletal muscle. PLoS Biology. 2017 Feb 16;15(2):e1002597. doi: 10.1371/journal.pbio.1002597.

Southwell AL, Skotte NH, Villanueva EB, Østergaard ME, Gu X, Kordasiewicz HB, Kay C, Cheung D, Xie Y, Waltl S, Dal Cengio L, Findlay-Black H, Doty CN, Petoukhov E, Iworima D, Slama R, Ooi J, Pouladi MA, Yang WX, Swayze EE, Seth PP, Hayden MR. A novel humanized mouse model of Huntington disease for preclinical development of therapeutics targeting mutant huntingtin alleles. Hum Mol Genet. 2017 Jan 18. pii: ddx021. doi: 10.1093/hmg/ddx021.

Warren HR, Evangelou E, Cabrera CP, Gao H, Ren M, Mifsud B, Ntalla I, Surendran P, Liu C, Cook JP, Kraja AT, Drenos F, Loh M, Verweij N, Marten J, Karaman I, Lepe MP, O'Reilly PF, Knight J, Snieder H, Kato N, He J, Tai ES, Said MA, Porteous D, Alver M, Poulter N, Farrall M, Gansevoort RT, Padmanabhan S, Mägi R, Stanton A, Connell J, Bakker SJ, Metspalu A, Shields DC, Thom S, Brown M, Sever P, Esko T, Hayward C, van der Harst P, Saleheen D, Chowdhury R, Chambers JC, Chasman DI, Chakravarti A, Newton-Cheh C, Lindgren CM, Levy D, Kooner JS, Keavney B, Tomaszewski M, Samani NJ, Howson JM, Tobin MD, Munroe PB, Ehret GB, Wain LV; International Consortium of Blood Pressure (ICBP) 1000G Analyses.; BIOS Consortium.; Lifelines Cohort Study.; Understanding Society Scientific group.; CHD Exome+ Consortium.; ExomeBP Consortium.; T2D-GENES Consortium.; GoT2DGenes Consortium.; Cohorts for Heart and Ageing Research in Genome Epidemiology (CHARGE) BP Exome Consortium.; International Genomics of Blood Pressure (iGEN-BP) Consortium.; UK Biobank CardioMetabolic Consortium BP working group. Genome-wide association analysis identifies novel blood pressure loci and offers biological insights into cardiovascular risk. Nat Genet. 2017 Mar;49(3):403-415. doi: 10.1038/ng.3768.

11. Manning A, Highland HM, Gasser J, …, Loh M, …, McCarthy MI, Glyon AL, Lindgren CM. A Low-Frequency Inactivating Akt2 Variant Enriched in the Finnish Population is Associated With Fasting Insulin Levels and Type 2 Diabetes Risk. Diabetes. 2017 Mar 24. pii: db161329. doi: 10.2337/db16-1329.

Böger CA, Gorski M, McMahon GM, …, Loh M, …, Snieder H, Parsa A, Cohen DM. NFAT5 and SLC4A10 are genetic loci regulating plasma osmolality. JASN. 2017 Mar 30. pii: ASN.2016080892. doi: 10.1681/ASN.2016080892..

Justice AE, Winkler TW, Feitosa MF, …, Loh M, …, Borecki IB, North KE, Cupples LA. Genome-Wide Meta-Analysis of 241,258 Adults Accounting for Smoking Behavior Identifies Novel Loci for Obesity Traits. Nature Communications. 2017 Apr 26;8:14977. doi: 10.1038/ncomms14977.

Yoon-Sim Yap, MD; Li-Lian Kwok, MSc; Nicholas Syn, MD; Wen Yee Chay, MD; John Whay Kuang Chia, MD; Chee Kian Tham, MD; Nan Soon Wong, MD; Soo Kien Lo, MD; Rebecca Alexandra Dent, MD; Sili Tan, BSc; Zuan Yu Mok, BSc; King Xin Koh, MSc; Han Chong Toh, MD; Wen Hsin Koo, MD; Marie Loh, PhD; Raymond Chee Hui Ng, MD; Su Pin Choo, MD; Richie Chuan Teck Soong, PhD. Predictors of Hand-Foot Syndrome and Pyridoxine for Prevention of Capecitabine–Induced Hand-Foot Syndrome. JAMA Oncology. 2017. Accepted.

Drögemöller, B. Monzon, J. Bhavsar, A. Borrie, A. Brooks, B. Wright, G. Liu, G. Fadhel, E. Renouf, D. Kollmannsberger, C. Bedard, P. Aminkeng, F. Hildebrand, C.,Gunaretnam, E. Critchley, C. Chen, Z. Brunham, LR. Hayden, M. Ross, C. Gelmon, K., Carleton, B. Genetic variation in SLC16A5 confers protection from cisplatin-induced ototoxicity in adult testicular cancer patients. Jama Oncology. 2017 Apr 27. doi: 10.1001/jamaoncol.2017.0502.

Ang HX, Chan SL, Sani LL, Quah CB, Brunham LR, Tan BOP, Winther MD. Development and validation of algorithms for the detection of statin myopathy signals from electronic medical records. Clin Pharmacol Ther. 2017 May;101(5):667-674. doi: 10.1002/cpt.526. Epub 2017 Jan 21..

Cirstea M, Walley KR, Russell JA, Brunham LR, Genga KR, Boyd JH. Pharmacogenomics in Asia: a systematic review on current trends and novel discoveries. Pharmacogenomics. 2017 Jun;18(9):891-910. doi: 10.2217/pgs-2017-0009. Epub 2017 Jun 8.

Woei-Yuh Saw, Erwin Tantoso, Husna Begum, Lihan Zhou, Ruiyang Zou, Cheng He, Sze Ling Chan, Linda Wei-Lin Tan, Lai Ping Wong, Wenting Xu, Don Kyin Nwe Moong, Yenly Lim, Bowen Li, E Nisha Pillai, Trevor Peterson, Tomasz Bielawny, Peter Meikle, Piyushukumar Mundra, Wei-Yen Lim, Ma Luo, Kee-Seng Chia, Rick Twee-Hee Ong, Liam Brunham, Chiea Chuen Khor, Heng Phon Too, Richie Soong, Markus Wenk, Peter Little, and Yik-Ying Teo. Establishing multiple omics baseline for three Southeast Asian ethnic groups in the Singapore Integrative Omics Cohort. Nature Communications. In Press.

Trigueros-Motos L, van Capelleveen JC, Torta F, Castaño D, Zhang LH, Chai C, Kang M, Dimova LG, Schimmel AWM, Tietjen I, Radomski C, Tan LJ, Hwee TC, Narayanaswamy P, Wu D, Dorninger F, Yakala GK, Barhdadi A, Angeli V, Dubé MP, Berger J, Dallinga-Thie GM, Tietge UJF, Wenk MR, Hayden MR, Hovingh GK, Singaraja RR. ABCA8 Regulates Cholesterol Efflux and High-Density Lipoprotein Cholesterol Levels. Arterioscler Thromb Vasc Biol. 2017 Sep 7. pii: ATVBAHA.117.309574. doi: 10.1161/ATVBAHA.117.309574.

2016

Garcia-Miralles M, Hong X, Tan LJ, Caron NS, Huang Y, To XV, Lin RY, Franciosi S, Papapetropoulos S, Hayardeny L, Hayden MR, Chuang KH, Pouladi MA. Laquinimod rescues striatal, cortical and white matter pathology and results in modest behavioural improvements in the YAC128 model of Huntington disease. Sci Rep. 2016 Aug 16;6:31652. doi: 10.1038/srep31652.

Itahana Y, Zhang J, Göke J, Vardy LA, Han R, Iwamoto K, Cukuroglu E, Robson P, Pouladi MA, Colman A, Itahana K. Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells. Sci Rep. 2016 Jun 27;6:28112. doi: 10.1038/srep28112.

Skotte NH*, Sanders SS*, Singaraja RR*, Ehrnhoefer DE, Vaid K, Qiu X, Kannan S, Verma C, Hayden MR. Palmitoylation of caspase-6 by HIP14 regulates its activation. Cell Death Differ. 2016 Dec 2. doi: 10.1038/cdd.2016.139.

Maillet A, Tan K, Chai X, Sadananda SN, Mehta A, Ooi J, Hayden MR, Pouladi MA, Ghosh S, Shim W, Brunham LR. Modeling doxorubicin-induced cardiotoxicity in human pluripotent stem cell derived-cardiomyocytes. Scientific Reports. 2016

Teo R, Hong X, Yu-Taeger L, Huang Y, Tan LJ, Xie Y, To XV, Guo L, Rajendran R, Novati A, Calaminus C, Riess O, Hayden MR, Nguyen HP, Chuang KH, Pouladi MA. Structural and molecular myelination deficits occur prior to neuronal loss in the YAC128 and BACHD models of Huntington disease. Human Molecular Genetics. 2016. doi: 10.1093/hmg/ddw122.

Garcia-Miralles M, Ooi J, Ferrari Bardile C, Tan LJ, George M, Drum CL, Lin RY, Hayden MR, Pouladi MA. Treatment with the MAO-A inhibitor clorgyline elevates monoamine neurotransmitter levels and improves affective phenotypes in a mouse model of Huntington disease. Experimental Neurology. 2016. doi:10.1016/j.expneurol.2016.01.019

Riechers SP, Butland S, Deng Y, Skotte N, Ehrnhoefer DE, Russ J, Laine J, Laroche M, Pouladi MA, Wanker E, Hayden MR, Graham RK. Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD. Human Molecular Genetics. 2016. doi: 10.1093/hmg/ddw036.

Chan SL, Samaranayake N, Ross CJ, Toh MT, Carleton B, Hayden MR, Teo YY, Dissanayake VH, Brunham LR. Genetic diversity of variants involved in drug response and metabolism in Sri Lankan populations: implications for clinical implementation of pharmacogenomics.Pharmacogenet Genomics. 2016 Jan;26(1):28-39. doi: 10.1097/FPC.0000000000000182.

Brunham LR. HDL as a Causal Factor in Atherosclerosis: Insights from Human Genetics. Curr Atheroscler Rep. 2016 Dec;18(12):71.

Chan SL, Tham M2, Tan SH, Loke C, Foo B, Fan Y, Ang PS, Brunham LR, Sung C. Development and validation of algorithms for the detection of statin myopathy signals from electronic medical records.Clin Pharmacol Ther. 2016 Oct 5. doi: 10.1002/cpt.526.

Chan SL, Ang X, Sani LL, Ng HY, Winther M2, Liu JJ, Brunham LR, Chan A. Prevalence and characteristics of adverse drug reactions at admission to hospital: a prospective observational study. Br J Clin Pharmacol. 2016 Sep 19. doi: 10.1111/bcp.13081.

Karaman I, Ferreira D, Boulange C, Kaluarachchi M, Herrington D, Dona A, Castagné R, Moayyeri A, Lehne B, Loh M, de Vries P, Dehghan A, Franco O, Hofman A, Evangelou E, Tzoulaki I, Elliott P, Lindon J, Ebbels T. A Workflow For Integrated Processing of Multi-Cohort Untargeted NMR Metabolomics Data In Large Scale Metabolic Epidemiology. Journal of Proteome Research. 2016.

Wahl S*, Drong A*, Lehne B*, Loh M*, Scott WR*, Kunze S, Tsai P-C, Ried JS, Zhang W, Yang Y, Tan S, Fiorito G, Franke L, Guarrera S, Kasela S, Kriebel J, Richmond RC, Adamo M, Afzal U, Ala-Korpela M, Albetti B, Ammerpohl O, Apperley JF, Beekman M, Bertazzi PA, Black S.L, Blancher C, Bonder M-J, Brosch M, Carstensen-Kirberg M, De Craen AJM, de Lusignan S, Dehghan A, Elkalaawy M, Fischer K, Franco OH, Gaunt TR, Hampe J, Hashemi M, Isaacs A, Jenkinson A, Jha S, Kato N, Krogh V, Laffan M, Meisinger C, Meitinger T, Mok ZY, Motta V, Ng HK, Nikolakopoulou Z, Nteliopoulos G, Panico S, Pervjakova N, Prokisch H, Rathmann W, Roden M, Rota F, Rozario MA, Sandling JK, Schafmayer C, Schramm K, Siebert R, Slagboom PE, Soininen P, Stolk L, Strauch K, Tai E-S, Tarantini L, Thorand B, Tigchelaar EF, Tumino R, Uitterlinden AG, van Duijn C, van Meurs JBJ, Vineis P, Wickremasinghe AR, Wijmenga C, Yang T-S, Yuan W, Zhernakova A, Batterham RL, Smith GD, Deloukas P, Heijmans BT, Herder C, Hofman A, Lindgren CM, Milani L, van der Harst P, Peters A, Illig T, Relton CL, Waldenberger M, Järvelin M-R, Bollati V, Soong R, Spector TD*, Bell JT*, Scott J*, McCarthy MI*, Elliott P*, Matullo G, Gieger C*, Kooner JS*, Grallert H*, Chambers JC*. Epigenome-wide association reveals extensive perturbations in DNA methylation associated with adiposity and its adverse metabolic consequences. Nature. 2016.

Lu Y, Day FR, Gustafsson S, …, Loh M, …, Lindgren C, Kilpeläinen TO, Loos RJF. New loci for body fat percentage reveal link between adiposity and cardiometabolic disease risk. Nat Commun. 2016 Feb 1;7:10495.

Scott WR, Zhang W, Loh M, Tan ST, Lehne B, Afzal U, Peralta J, Saxena R, Ralhan S, Wander GS, Bozaoglu K, Sanghera DK, Elliott P, Scott J, Chambers JC, Kooner JS. Investigation of Genetic Variation Underlying Central Obesity amongst South Asians. PLoS One. 2016 May 19;11(5):e0155478. doi: 10.1371/journal.pone.0155478.

Lehne B, Drong AW, Loh M, Zhang W, Scott WR, Tan ST, Afzal U, Schulz R, Scott J, Jarvelin MR, Elliott P, McCarthy MI, Kooner JS, Chambers JC. Erratum to: A coherent approach for analysis of the Illumina HumanMethylation450 BeadChip improves data quality and performance in epigenome-wide association studies. Genome Biol. 2016 Apr 21;17:73. doi: 10.1186/s13059-016-0934-z.

Soo RA, Syn N, Lee SC, Wang L, Lim XY, Loh M, Tan SH, Zee YK, Wong AL, Chuah B, Chan D, Lim SE, Goh BC, Soong R, Yong WP. Pharmacogenetics-Guided Phase I Study of Capecitabine on an Intermittent Schedule in Patients with Advanced or Metastatic Solid Tumours. Sci Rep. 2016 Jun 14;6:27826. doi: 10.1038/srep27826.

Halliwell B, Cheah IK, Drum CL. Ergothioneine, an adaptive antioxidant for the protection of injured tissues? A hypothesis. Biochem Biophys Res Commun. 2016 Feb 5;470(2):245-50. doi: 10.1016/j.bbrc.2015.12.124.

Patra A, Ding T, Engudar G, Wang Y, Dykas MM, Liedberg B, Kah JC, Venkatesan T, Drum CL. Component-Specific Analysis of Plasma Protein Corona Formation on Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance. Small. 2016 Mar 2;12(9):1174-82. doi: 10.1002/smll.201501603.

2015

Sanders SS, Hou J, Sutton LM, Garside VC, Mui KK, Singaraja RR, Hayden MR, Hoodless PA. Huntingtin interacting proteins 14 and 14-like are required for chorioallantoic fusion during early placental development. Dev Biol. 2015 Jan 15;397(2):257-66. 

Sadananda SN, Foo JN, Toh MT, Cermakova L, Trigueros-Motos L, Chan T, Liany H, Collins JA, Gerami S, Singaraja RR, Hayden MR, Francis GA, Frohlich J, Khor CC, Brunham LR. Targeted next-generation sequencing to diagnose disorders of HDL cholesterol. J Lipid Res. 2015 Oct;56(10):1993-2001. doi: 10.1194/jlr.P058891. Epub 2015 Aug 8.

Wong BKY, Ehrnhoefer DE, Graham RK, Martin DD, Ladha S, Uribe V, Stanek LM, Franciosi S, Qiu X, Deng Y, Kovalik V, Zhang W, Pouladi MA, Shihabuddin LS, Hayden MR. Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice.  Neurobiology of Disease. 2015. In Press.

Tan SH, Sapari NS, Miao H, Hartman M, Loh M, Chng WJ, Iau P, Buhari SA, Soong R, Lee SC. High-Throughput Mutation Profiling Changes before and 3 Weeks after Chemotherapy in Newly Diagnosed Breast Cancer Patients. PLoS One. 2015 Dec 2;10(12):e0142466.

Kato N*, Loh M*, Takeuchi F*, …, van der Harst P*, Kooner JS*, Chambers JC*. Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation. Nat Genet. 2015 Sep 21. (*: Contributed equally)

Joshi PK, Esko T, Mattsson H, …, Loh M, …, Perola M, Polašek O, Wilson JF, for ROHgen. Directional dominance on stature and cognition in diverse human populations. Nature. 2015 Jul 23;523(7561):459-62.

Chambers JC*, Loh M*, Lehne B*, …, McCarthy MI*, Elliott P*, Kooner JS*. Epigenome-wide association of DNA methylation markers in peripheral blood from Indian Asians and Europeans with incident type 2 diabetes: a nested case-control study. Lancet Diabetes Endocrinol. 2015 Jul;3(7):526-34. (*: Contributed equally)

Chan SL, Jin S, Loh M, Brunham LR. Progress in understanding the genomic basis for adverse drug reactions: a comprehensive review and focus on the role of ethnicity. Pharmacogenomics. 2015 Jul;16(10):1161-78.

Lehne B*, Drong A*, Loh M, Zhang W, Scott WR, Tan S-T, Afzal U, Blancher C, Scott J, Jarvelin M-R, Elliott P, McCarthy MI, Kooner JS, Chambers JC. A coherent approach for analysis of the Illumina HumanMethylation450 BeadChip improves data quality and performance in epigenome-wide association studies. Genome Biol. 2015 Feb 15;16(1):37.

Mahajan A, Sim X, Ng HJ, …, on behalf of the T2D-GENES consortium and GoT2D consortium. Identification and functional characterization of G6PC2 coding variants influencing glycemic traits defines effector transcript at the G6PC2-ABCB11 locus.PLoS Genet. 2015 Jan 27;11(1):e1004876. 

Patra A, Ding T, Engudar G, Yi W, Dykas M, Liedberg B, Kah J, Venkatesan T, Drum CL. Component Specific Analysis of Plasma Protein Corona Formation On Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance. Small. 2015 (selected for early online publication, Sept 10, 2015): doi: 10.1002/smll.201501603

Ding T, Hong M, Richards AM, It Wong Ten, Zhou X, Drum CL. Quantification of a Cardiac Biomarker in Human Serum Using Extraordinary Optical Transmission (EOT). PLoS ONE. 2015 Mar 16;10(3):e0120974.

Drum CL. System-level prescriptions for adaptive drug licensing. Science Translational Medicine. 2015 Mar 4;7(277):277ec36.

Chan JM, Wong KHK, Richards AM, Drum CL. Microengineering in cardiovascular research: new developments and translational applications. cardiovascular research. 2015 Feb 17. (editor’s choice)

Drum CL. TRIPOD puts prediction models on a firmer footing. Science Translational Medicine. 2015 Jan 28;7(272):272ec14–4.

2014

Ooi J, Hayden MR, Pouladi MA. Inhibition of excessive monoamine oxidase A/B activity protects against increased stress-induced neuronal death in Huntington disease. Molecular Neurobiology. 2014. doi: 10.1007/s12035-014-8974-4  PDF

Herr KJ, Tsang YH, Ong JW, Li Q, Yap LL, Yu W, Yin H, Bogorad RL, Dahlman JE, Chan YG, Bay BH, Singaraja RR, Anderson DG, Koteliansky V, Viasnoff V, Thiery JP. Loss of α-catenin elicits a cholestatic response and impairs liver regeneration. Sci Rep. 2014 Oct 30;4:6835. 

Kaur A, Patankar JV, de Haan W, Ruddle P, Wijesekara N, Groen AK, Verchere CB, Singaraja RR, Hayden MR. Loss of Cyp8b1 improves glucose homeostasis by increasing GLP-1. Diabetes. 2014 Oct 22. pii: DB_140716.


Butland SL, Sanders SS, Schmidt ME, Riechers SP, Lin DT, Martin DD, Vaid K, Graham RK, Singaraja RR, Wanker EE, Conibear E, Hayden MR. The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease. Hum Mol Genet. 2014 Aug 1;23(15):4142-60. 

Singaraja RR, Tietjen I, Hovingh GK, Franchini PL, Radomski C, Wong K, vanHeek M, Stylianou IM, Lin L, Wang L, Mitnaul L, Hubbard B, Winther M, Mattice M, Legendre A, Sherrington R, Kastelein JJ, Akinsanya K, Plump A, Hayden MR. Identification of four novel genes contributing to familial elevated plasma HDL cholesterol in humans. J Lipid Res. 2014;55(8):1693-1701.

Rinninger F, Heine M, Singaraja RR, Hayden M, Brundert M, Ramakrishnan R, Heeren J. High Density Lipoprotein Metabolism in Low Density Lipoprotein Receptor-deficient Mice. J Lipid Res. 2014. In Press

Bochem AE, van Capelleveen JC, Dallinga-Thie GM, Schimmel AW, Motazacker MM, Tietjen I, Singaraja RR, Hayden MR, Kastelein JJ, Stroes ES, Hovingh GK. Two novel mutations in apolipoprotein C3 underlie atheroprotective lipid profiles in families. Clin Genet. 2014;85(5):433-40. 

Philips T, Rothstein JD, Pouladi MA. Preclinical models needed in translation? A Pro/Con debate.Movement Disorders Journal. 2014. 29: 1391-1396. 

Sapari NS, Elahi E, Wu M, Loh M, Ng HK, Han X, Yap HL, Klemm TP, Pang B, Benoukraf T, Teo YY, Iacopetta B, Lee SC, Soong R. Feasibility of low-throughput next generation sequencing for germline DNA screening. Clin Chem. 2014 Dec;60(12):1549-57.

Chambers JC, Abbott J, Zhang W, Turro E, Scott WR, Tan S-T, Loh M, Lehne B, O'Reilly P, Gaulton KJ, Pearson RD, Li X, Lavery A, Vandrovcova J, Wass MN, Miller K, Sehmi J, Oozageer L, Kooner I, Al-Hussaini A, Afzal U, Mills R, Grewal J, Panoulas V, Lewin AM, Northwood K, Wander GS, Geoghegan F, Li Y, Wang J, Aitman TJ, McCarthy MI, Scott J, Butcher S, Elliott P, Kooner JS. The South Asian Genome. PLoS ONE 2014 Aug 12;9(8):e102645. doi: 10.1371/journal.pone.0102645. 

Loh M, Liem N, Vaithilingam A, Lim PL, Sapari NS, Cheng CL, Yan B, Pang B, Salto-Tellez M, Yong WP, Iacopetta B, Soong R. DNA Methylation Subgroups and the CpG Island Methylator Phenotype in Gastric Cancer: A Comprehensive Profiling Approach. BMC Gastroenterol. 2014 Mar 28;14:55. doi: 10.1186/1471-230X-14-55.

Chong ML, Loh M, Thakkar B, Pang B, Iacopetta B, Soong R. Phosphatidylinositol-3-kinase Pathway Aberrations in Gastric and Colorectal Cancer: Meta-analysis, Co-occurrence and Ethnic Variation. Int J Cancer. 2014 Mar 1; 134(5):1232-8. doi: 10.1002/ijc.28444.

Subramaniam MM*, Loh M*, Chan JY, Liem N, Lim PL, Yong WP, Lim XY, Yeoh KG, Iacopetta B, Soong R and Salto-Tellez M. The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers. Mol Carcinog. 2014 Feb; 53(2):98-108. doi: 10.1002/mc.21951. (*: co-first author)

Drum CL. Watch your leftovers: Remnant cholesterol brings oversized risk to cardiovascular health. Science Translational Medicine. 2014 Dec 3;6(265):205.

Drum CL. Short circuiting mitochondria leads to improvement in mouse models of diabetes. Science Translational Medicine. 2014 Oct 22;6(259):179.

Drum CL. The ubiquitinase pathway takes center stage in viral myocarditis. Science Translational Medicine. 2014 Sep 10;6(253):155.

Yu J, Tao D, Ng EX, Drum CL, Liu AQ, Chen C-H. Real-time measurement of thrombin generation using continuous droplet microfluidics. Biomicrofluidics. 2014 Sep;8(5):052108.

Drum CL. Long non-coding RNAs in myocardial infarction. Science Translational Medicine. 2014 Jul 30;6(247):131.

Drum CL. Taking Cancer Cells Out of Circulation. Science Translational Medicine. 2014 Jun 18;6(241):104.

Drum CL. Magnetic Resonance: It’s a Gas! Science Translational Medicine. 2014 May 7;6(235):80.

Drum CL. Getting to the Heart of Consciousness. Science Translational Medicine. 2014 Mar 26;6(229):53.

2013

Brunham LR, Hayden MR. Hunting human disease genes: lessons from the past, challenges for the future. Human genetics. Mar 17 2013.

Milnerwood AJ, Parsons MP, Young FB, Singaraja RR, Franciosi S, Volta M, Bergeron S, Hayden MR, Raymond LA. Memory and synaptic deficits in Hip14/DHHC17 knockout mice. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20296-301. doi: 10.1073/pnas.1222384110. Epub 2013 Nov 25.

Wan J, Savas JN, Roth AF, Sanders SS, Singaraja RR, Hayden MR, Yates JR 3rd, Davis NG. Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease. Chem Biol. 2013 Nov 21;20(11):1421-34. doi: 10.1016/j.chembiol.2013.09.018. Epub 2013 Nov 7.

Wu DH, Singaraja RR. Loss-of-function mutations in CCDC114 cause primary ciliary dyskinesia. Clin Genet. 2013 Jun;83(6):526-7. doi: 10.1111/cge.12127. Epub 2013 Mar 18.

Chai EC, Singaraja RR. Opsismodysplasia: implications of mutations in the developmental gene INPPL1. Clin Genet. 2013 Jun;83(6):527-9. doi: 10.1111/cge.12136. Epub 2013 Mar 24.

Singaraja RR. TREM2: a new risk factor for Alzheimer's disease. Clin Genet. 2013 Jun;83(6):525-6. doi: 10.1111/cge.12108.

Sutton LM, Sanders SS, Butland SL, Singaraja RR, Franciosi S, Southwell AL, Doty CN, Schmidt ME, Mui KK, Kovalik V, Young FB, Zhang W, Hayden MR. Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease. Hum Mol Genet. 2013 Feb 1;22(3):452-65. doi: 10.1093/hmg/dds441. Epub 2012 Oct 16.

Singaraja RR, Sivapalaratnam S, Hovingh K, Dubé MP, Castro-Perez J, Collins HL, Adelman SJ, Riwanto M, Manz J, Hubbard B, Tietjen I, Wong K, Mitnaul LJ, van Heek M, Lin L, Roddy TA, McEwen J, Dallinge-Thie G, van Vark-van der Zee L, Verwoert G, Winther M, van Duijn C, Hofman A, Trip MD, Marais AD, Asztalos B, Landmesser U, Sijbrands E, Kastelein JJ, Hayden MR. The impact of partial and complete loss-of-function mutations in endothelial lipase on high-density lipoprotein levels and functionality in humans. Circ Cardiovasc Genet. 2013;6(1):54-62.

Pouladi MA, Morton AJ, Hayden MR. Choosing an Animal Model for the Study of Huntington Disease. Nature Reviews Neuroscience. 2013. 14: 708–721.

Marco S, Giralt A, Petrovic MM, Pouladi MA, Martínez-Turrillas R, Martínez-Hernández J, Kaltenbach LS, Torres-Peraza J, Graham RK, Watanabe M, Luján R, Nakanishi N, Lipton SA, Lo DC, Hayden MR, Alberch J, Wesseling JF, Pérez-Otaño I. Suppressing aberrant GluN3A expression rescues NMDA receptor dysfunction, synapse loss and motor and cognitive decline in Huntington’s disease models. Nature Medicine. 2013. 19: 1030-8.

2012

Brunham LR, Hayden MR. Medicine. Whole-genome sequencing: the new standard of care? Science. Jun 1 2012;336(6085):1112-1113.

Brunham LR, Lansberg PJ, Zhang L, Miao F, Carter C, Hovingh GK, Visscher H, Jukema JW, Stalenhoef AF, Ross CJ, Carleton BC, Kastelein JJ, Hayden MR. Differential effect of the rs4149056 variant in SLCO1B1 on myopathy associated with simvastatin and atorvastatin. Pharmacogenomics J. Jun 2012;12(3):233-237.

Kruit JK, Wijesekara N, Westwell-Roper C, Vanmierlo T, de Haan W, Bhattacharjee A, Tang R, Wellington CL, LütJohann D, Johnson JD, Brunham LR, Verchere CB, Hayden MR. Loss of both ABCA1 and ABCG1 results in increased disturbances in islet sterol homeostasis, inflammation, and impaired beta-cell function. Diabetes. Mar 2012;61(3):659-664.

Graham RK, Deng Y, Pouladi MA, Vaid K, Ehrnhoefer DE, Southwell AL, Bissada N, Franciosi S, Hayden MR. Caspase-6-resistant mutant huntingtin does not rescue the toxic effects of caspase-cleavable mutant huntingtin in vivo.Journal of Huntington’s disease. 2012. doi:10.3233/JHD-120038.

Southwell AL, Warby SC, Carroll JB, Doty CN, Skotte NH, Zhang WN, Villanueva EB, Kovalik V, Xie Y, Pouladi MA, Collins JA, Yang W, Franciosi S, Hayden MR. A fully humanized transgenic mouse model of Huntington disease. Human Molecular Genetics. 22: 18-34.

Pouladi MA, Brillaud E, Xie Y, Conforti P, Graham RK, Ehrnhoefer DE, Franciosi S, Zhang W, Zapala M, Poucheret P, Compte E, Maurel J-C, Zuccato C, Cattaneo E, Neri C, Hayden MR. NP03,a novel low-dose lithium formulation, is neuroprotective in the YAC128 mouse model of Huntington disease. Neurobiology of Disease. 2012. 48: 282-289.

Pouladi MA, Stanek LM, Xie Y, Franciosi S, Deng Y, Butland S, Warby S, Zhang W, Cheng SH, Shihabuddin LS, Hayden MR. Marked differences in neurochemistry and aggregates despite similar behavioural and neuropathological features of Huntington disease in the full-length BACHD and YAC128 mice. Human Molecular Genetics. 2012. 21: 2219-2232.

Huang Y, Pouladi MA. Heritability of pain sensitivity and opioid analgesia.Clin Genet. 2012. 82: 376-7.

Teo R, Pouladi MA. PS: Pain and sodium channels. Clin Genet. 2012. 82: 374-6.

Pouladi MA. Painful gains: missense mutations in SCN9A and idiopathic small nerve fibre neuropathy. Clin Genet. 2012. 82: 374.

Uribe V, Wong BKY, Graham RK, Cusack CL, Skotte NH, Pouladi MA, Xie Y, Deng Y, Ehrnhoefer DE, Franciosi S, Bissada N, Spreeuw A, Zhang W, Vaid K, Deshmukh M, Howland D, Hayden MR. Rescue from excitotoxicity and axonal degeneration accompanied by age-dependent behavioral and neuroanatomical alterations in caspase-6-deficient mice. Human Molecular Genetics. 2012. 21: 1954-67.

Tietjen I, Hovingh GK, Singaraja RR, Radomski C, Barhdadi A, McEwen J, Chan E, Mattice M, Legendre A, Franchini PL, Dubé MP, Kastelein JJ, Hayden MR. Segregation of LIPG, CETP, and GALNT2 mutations in Caucasian families with extremely high HDL cholesterol. PLoS One. 2012;7(8):e37437.

Tietjen I, Hovingh GK, Singaraja RR, Radomski C, McEwen J, Chan E, Mattice M, Legendre A, Kastelein JJ, Hayden MR. Increased risk of coronary artery disease in Caucasians with extremely low HDL cholesterol due to mutations in ABCA1, APOA1, and LCAT. Biochim Biophys Acta. 2012;1821(3):416-24.

Young FB, Franciosi S, Spreeuw A, Deng Y, Sanders S, Tam NC, Huang K, Singaraja RR, Zhang W, Bissada N, Kay C, Hayden MR. Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice. PLoS One. 2012;7(5):e36315. doi: 10.1371/journal.pone.0036315. Epub 2012 May 23.

Loh M, Chua D, Yao Y, Soo RA, Garrett K, Zeps N, Platell C, Minamoto T, Kawakami K, Iacopetta B and Soong R. Can population differences in chemotherapy outcomes be inferred from differences in pharmacogenetic frequencies? The Pharmacogenomics Journal 2012 June 26. doi: 10.1038/tpj.2012.26. 

Sapari NS, Loh M, Vaithilingam A and Soong R. Clinical Potential of DNA Methylation in Gastric Cancer: A Meta-Analysis. PLoS ONE 7(4):e36275. doi: 10.1371/journal.pone.0036275.

Soo RA, Kawaguchi T, Loh M, Ou S-HI, Shieh MP, Cho B-C and Soong R. Differences in outcome and toxicity between Asian and Caucasian patients with lung cancer treated with systemic therapy. Future Oncology 2012 Apr; 8(4):451-62. doi: 10.2217/fon.12.25. 

Eichler H-G, Oye K, Baird LG, Abadie E, Brown J, Drum CL, et al. Adaptive licensing: taking the next step in the evolution of drug approval. Clin Pharmacol Ther. 2012 Mar;91(3):426–37.

De Sousa FB, Lima AC, Denadai AML, Anconi CPA, De Almeida WB, Novato WTG, et al. Superstructure based on β-CD self-assembly induced by a small guest molecule. Phys Chem Chem Phys. The Royal Society of Chemistry; 2012 Feb 14;14(6):1934–44.

2011

Brunham LR, Tietjen I, Bochem AE, Singaraja RR, Franchini PL, Radomski C, Mattice M, Legendre A, Hovingh GK, Kastelein JJ, Hayden MR. Novel mutations in scavenger receptor BI associated with high HDL cholesterol in humans. Clin Genet. 2011;79(6):575-81.

Singaraja RR, Huang K, Sanders SS, Milnerwood AJ, Hines R, Lerch JP, Franciosi S, Drisdel RC, Vaid K, Young FB, Doty C, Wan J, Bissada N, Henkelman RM, Green WN, Davis NG, Raymond LA, Hayden MR. Altered palmitoylation and neuropathological deficits in mice lacking HIP14. Hum Mol Genet. 2011 Oct 15;20(20):3899-909. doi: 10.1093/hmg/ddr308. Epub 2011 Jul 20.

Ye D, Zhao Y, Hildebrand RB, Singaraja RR, Hayden MR, Van Berkel TJ, Van Eck M. The dynamics of macrophage infiltration into the arterial wall during atherosclerotic lesion development in low-density lipoprotein receptor knockout mice. Am J Pathol. 2011 Jan;178(1):413-22. doi: 10.1016/j.ajpath.2010.11.007. Epub 2010 Dec 23.

Song W, Chen J, Petrilli A, Liot G, Klinglmayr E, Zhou Y, Poquiz P, Tjong J, Pouladi MA, Hayden MR, Masliah E, Ellisman M, Rouiller I, Schwarzenbacher R, Bossy B, Perkins G, Bossy-Wetzel E. Mutant huntingtin binds the mitochondrial fission GTPase dynamin-related protein-1 and increases its enzymatic activity. Nature Medicine. 2011. 17 (3): 377-382.

Simpson JM, Gil-Mohapel JM, Pouladi MA, Ghilan M, Xie Y, Hayden MR, Christie BR. Altered adult hippocampal neurogenesis in the YAC128 transgenic mouse model of Huntington disease. Neurobiol Dis. 2011. 41: 249-60.

Loh M and Soong R. Challenges and Pitfalls in the Introduction of Pharmacogenetics for Cancer. Ann Acad Med Singapore 2011; 40(8):369-374.

Soo RA*, Loh M*, Mok T, Ou S-HI , Cho BC, Yeo WL, Tenen DG and Soong R. Ethnic differences in survival outcome in patients with advanced stage non-small cell lung cancer- results of a meta-analysis of randomised controlled trials. Journal of Thoracic Oncology 2011 Apr 28 Jun; 6(6):1030-1038.  doi: 10.1097/JTO.0b013e3182199c03. (*: co-first author)

Tan WL, Bhattacharya B, Loh M, Balasubramanian I, Akram M, Dong D, Wong L, Thakkar B, Salto-Tellez M, Soo RA, Fichtner I, Iacopetta B and Soong R. Low cytosine triphosphate synthase 2 expression renders resistance to 5-fluorouracil in colorectal cancer. Cancer Biology & Therapy 2011 Mar 15; 11(6):599-608.

Chan JM, Rhee J-W, Drum CL, Bronson RT, Golomb G, Langer R, et al. In vivo prevention of arterial restenosis with paclitaxel-encapsulated targeted lipid-polymeric nanoparticles. Proc Natl Acad Sci USA. National Acad Sciences; 2011 Nov 29;108(48):19347–52.

2010

Kng MH, Singaraja R, Hayden MR. Adenosine-triphosphate-binding cassette transporter-1 trafficking and function. Trends Cardiovasc Med. 2010;20(2):41-9.

Chung S, Timmins JM, Duong M, Degirolamo C, Rong S, Sawyer JK, Singaraja RR, Hayden MR, Maeda N, Rudel LL, Shelness GS, Parks JS. Targeted deletion of hepatocyte ABCA1 leads to very low density lipoprotein triglyceride overproduction and low density lipoprotein hypercatabolism. J Biol Chem. 2010;285(16):12197-209.

Vergeer M, Brunham LR, Koetsveld J, Kruit JK, Verchere CB, Kastelein JJ, Hayden MR, Stroes ES. Carriers of loss-of-function mutations in ABCA1 display pancreatic beta-cell dysfunction. Diabetes Care. Apr 2010;33(4):869-874.

Graham RK, Deng Y, Carroll J, Vaid K, Cowan C, Pouladi MA, Metzler M, Bissada N, Wang L, Faull RL, Gray M, Yang XW, Raymond LA, Hayden MR. Cleavage at the 586 amino acid caspase-6 site in mutant huntingtin influences caspase-6 activation in vivo. J Neurosci. 2010. 30: 15019-15029.

Valenza M, Leoni V, Karasinska JM, Petricca L, Fan J, Carroll J, Pouladi MA, Fossale E, Nguyen HP, Riess O, MacDonald M, Wellington C, DiDonato S, Hayden M, Cattaneo E. Cholesterol defect is marked across multiple rodent models of Huntington's disease and is manifest in astrocytes.J Neurosci. 2010. 30: 10844-10850.

Milnerwood AJ, Gladding CM, Pouladi MA, Kaufman AM, Hines RM, Boyd JD, Ko RW, Vasuta OC, Graham RK, Hayden MR, Murphy TH, Raymond LA. Early Increase in Extrasynaptic NMDA Receptor Signaling and Expression Contributes to Phenotype Onset in Huntington’s Disease Mice. Neuron. 2010. 65: 178–190.

Pouladi MA, Xie Y, Skotte NH, Ehrnhoefer DE, Graham RK, Kim JE, Bissada N, Yang XW, Paganetti P, Friedlander RM, Leavitt BR, Hayden MR. Full-length huntingtin levels modulate body weight by influencing insulin-like growth factor 1 expression.Human Molecular Genetics. 2010. 19: 1528-1538.

Becanovic K, Pouladi MA, Lim RS, Kuhn A, Pavlidis P, Luthi-Carter R, Hayden MR, Leavitt BR. Transcriptional changes in HD identified using genome-wide expression profiling and cross platform analysis.Hum Mol Genet. 2010. 19: 1438–1452.

Loh M, Liem N, Lim PL, Vaithilingam A, Cheng CL, Salto-Tellez M, Yong WP and Soong R. Impact of Sample Heterogeneity on Methylation Analysis. Diagnostic Molecular Pathology 2010 Dec; 19(4):243-7. doi: 10.1097/PDM.0b013e3181de4396.

Ang PW, Loh M, Liem N, Lim PL, Grieu F, Vaithilingam A, Platell C, Yong WP, Iacopetta B and Soong R. Comprehensive profiling of DNA methylation in colorectal cancer reveals subgroups with distinct clinicopathological and molecular features. BMC Cancer. 2010 May 21; 10:227. doi: 10.1186/1471-2407-10-227.

Lai KW, Koh KX, Loh M, Tada K, Subramaniam MM, Lim XY, Vaithilingam A, Salto-Tellez M, Iacopetta B, Ito Y, Soong R and the Singapore Gastric Cancer Consortium. MicroRNA-130b regulates the tumour suppressor RUNX3 in gastric cancer. Eur J Cancer. 2010 May; 46(8):1456-63. doi: 10.1016/j.ejca.2010.01.036. 

2009

Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 2009;105(2):138-47.

Karasinska JM, Rinninger F, Lütjohann D, Ruddle P, Franciosi S, Kruit JK, Singaraja RR, Hirsch-Reinshagen V, Fan J, Brunham LR, Bissada N, Ramakrishnan R, Wellington CL, Parks JS, Hayden MR. Specific loss of brain ABCA1 increases brain cholesterol uptake and influences neuronal structure and function. J Neurosci. 2009;29(11):3579-89.

Warby SC, Doty CN, Graham RK, Shively J, Singaraja RR, Hayden MR. Phosphorylation of huntingtin reduces the accumulation of its nuclear fragments.Mol Cell Neurosci. 2009 Feb;40(2):121-7. doi: 10.1016/j.mcn.2008.09.007. Epub 2008 Oct 18.

Brunham LR, Singaraja RR, Duong M, Timmins JM, Fievet C, Bissada N, Kang MH, Samra A, Fruchart JC, McManus B, Staels B, Parks JS, Hayden MR. Tissue-specific roles of ABCA1 influence susceptibility to atherosclerosis.  Arterioscler Thromb Vasc Biol. 2009;29(4):548-54.

Okamoto S*, Pouladi MA*, Talantova M*, Yao D, Xia P, Ehrnhoefer DE, Zaidi R, Clemente A, Kaul M, Graham RK, Zhang D, Vincent Chen HS, Tong G, Hayden MR, Lipton SA. Balance between synaptic versus extrasynaptic NMDA receptor activity influences inclusions and neurotoxicity of mutant huntingin.Nature Medicine. 2009. 15: 1407–1413. (* equal contribution)

Pouladi MA, Graham RK, Karasinska JM, Xie Y, Santos RD, Petersén A, Hayden MR. Prevention of depressive behaviour in the YAC128 mouse model of Huntington disease by mutation at residue 586 of huntingtin. Brain. 2009. 132: 919-32

Graham RK, Pouladi MA, Joshi P, Lu G, Deng Y, Wu NP, Figueroa BE, Metzler M, André VM, Slow EJ, Raymond L, Friedlander R, Levine MS, Leavitt BR, Hayden MR. Differential susceptibility to excitotoxic stress in YAC128 mouse models of HD between initiation and progression of disease. J Neurosci. 2009. 29: 2193-204.

Maconald ML, van Eck M, Hildebrand RB, Wong BW, Bissada N, Ruddle P, Kontush A, Hussein H, Pouladi MA, Chapman MJ, Fievet C, van Berkel TJ, Staels B, McManus BM, Hayden MR. Despite Antiatherogenic Metabolic Characteristics, SCD1-Deficient Mice Have Increased Inflammation and Atherosclerosis. Arterioscler Thromb Vasc Biol. 2009. 29: 341-7.

Loh M, Koh KX, Yeo BH, Song CM, Chia KS, Zhu F, Yeoh KG, Hill J, Iacopetta B and Soong R. Meta-analysis of genetic polymorphisms and gastric cancer risk: Variability in associations according to race. European Journal of Cancer 2009 Sep; 45(14):2562-8. doi: 10.1016/j.ejca.2009.03.017.

Zhu F, Loh M, Hill J, Lee S, Koh KX, Lai KW, Salto-Tellez M, Iacopetta B, Yeoh KG, Soong R and the Singapore Gastric Cancer Consortium. Genetic factors associated with intestinal metaplasia in a high risk Singapore-Chinese population: a cohort study. BMC Gastroenterology. 2009 Oct 13; 9:76. doi: 10.1186/1471-230X-9-76.

2008

Brunham LR, Kastelein JJ, Hayden MR. ABCA1 gene mutations, HDL cholesterol levels, and risk of ischemic heart disease. JAMA. Nov 5 2008;300(17):1997-1998; author reply 1998.

Brunham LR, Kruit JK, Verchere CB, Hayden MR. Cholesterol in islet dysfunction and type 2 diabetes. J Clin Invest. Feb 2008;118(2):403-408.

Brunham LR, Kruit JK, Pape TD, et al. Beta-cell ABCA1 influences insulin secretion, glucose homeostasis and response to thiazolidinedione treatment. Nat Med. Mar 2007;13(3):340-347.

Hines RM, Wu L, Hines DJ, Steenland H, Mansour S, Dahlhaus R, Singaraja RR, Cao X, Sammler E, Hormuzdi SG, Zhuo M, El-Husseini A. Synaptic imbalance, stereotypies, and impaired social interactions in mice with altered neuroligin 2 expression. J Neurosci. 2008 Jun 11;28(24):6055-67. doi: 10.1523/JNEUROSCI.0032-08.2008.

Warby SC, Doty CN, Graham RK, Carroll JB, Yang YZ, Singaraja RR, Overall CM, Hayden MR. Activated caspase-6 and caspase-6-cleaved fragments of huntingtin specifically colocalize in the nucleus. Hum Mol Genet. 2008 Aug 1;17(15):2390-404. doi: 10.1093/hmg/ddn139. Epub 2008 Apr 29.

MacDonald ML, Singaraja RR, Bissada N, Ruddle P, Watts R, Karasinska JM, Gibson WT, Fievet C, Vance JE, Staels B, Hayden MR. Absence of stearoyl-CoA desaturase-1 ameliorates features of the metabolic syndrome in LDLR-deficient mice. J Lipid Res. 2008 Jan;49(1):217-29. Epub 2007 Oct 24.

Björkqvist M, Wild EJ, Thiele J, Silvestroni A, Andre R, Lahiri N, Raibon E, Lee RV, Benn CL, Soulet D, Magnusson A, Woodman B, Landles C, Pouladi MA, Hayden MR, Khalili-Shirazi A, Lowdell MW, Brundin P, Bates GP, Leavitt BR, Möller T, Tabrizi SJ. A novel pathogenic pathway of immune activation detectable before clinical onset in Huntington’s disease. J Exp Med. 2008. 205: 1869-77.

Wong WC, Loh M and Eisenhaber F. On the necessity of different statistical treatment for Illumina BeadChip and Affymetrix GeneChip data and its significance for biological interpretation. Biology Direct 2008 Jun 3;3(1):23. doi: 10.1186/1745-6150-3-23.

2007

Kuhn A, Goldstein DR, Hodges A, Strand AD, Sengstag T, Kooperberg C, Becanovic K, Pouladi MA, Sathasivam K, Cha JH, Hannan AJ, Hayden MR, Leavitt BR, Dunnett SB, Ferrante RJ, Albin R, Shelbourne P, Delorenzi M, Augood SJ, Faull RL, Olson JM, Bates GP, Jones L, Luthi-Carter R. Mutant huntingtin's effects on striatal gene expression in mice recapitulate changes observed in human Huntington's disease brain and do not differ with mutant huntingtin length or wild-type huntingtin dosage. Human Molecular Genetics. 2007. 16: 1845-61.

Brunham LR, Kruit JK, Pape TD, Timmins JM, Reuwer AQ, Vasanji Z, Marsh BJ, Rodrigues B, Johnson JD, Parks JS, Verchere CB, Hayden MR. Beta-cell ABCA1 influences insulin secretion, glucose homeostasis and response to thiazolidinedione treatment. Nat Med. Mar 2007;13(3):340-347.

Vaid KS, Guttman JA, Singaraja RR, Vogl AW. A kinesin is present at unique sertoli/spermatid adherens junctions in rat and mouse testes. Biol Reprod. 2007 Dec;77(6):1037-48. Epub 2007 Sep 12.

Valenza M, Carroll JB, Leoni V, Bertram LN, Björkhem I, Singaraja RR, Di Donato S, Lutjohann D, Hayden MR, Cattaneo E. Cholesterol biosynthesis pathway is disturbed in YAC128 mice and is modulated by huntingtin mutation. Hum Mol Genet. 2007 Sep 15;16(18):2187-98. Epub 2007 Jul 5.

Hirsch-Reinshagen V, Chan JY, Wilkinson A, Tanaka T, Fan J, Ou G, Maia LF, Singaraja RR, Hayden MR, Wellington CL. Physiologically regulated transgenic ABCA1 does not reduce amyloid burden or amyloid-beta peptide levels in vivo. J Lipid Res. 2007 Apr;48(4):914-23. Epub 2007 Jan 18.

Choong LY, Lim S, Loh MCS, Man X, Chen Y, Toy W, Pan M, Chen CS, Poonepalli A, Hande MP, Tan PH, Salto-Tellez M, Wong CY, Shah N, Druker BJ and Lim YP. Progressive loss of EGFR in a sub-population of breast cancers - implications in target-directed therapeutics. Molecular Cancer Therapeutics 2007 Nov;6(11):2828-42. 

Tan SH, Ida H, Lau QC, Goh BC, Chieng WS, Loh M and Ito Y. Detection of Promoter Hypermethylation in Serum Samples of Cancer Patients by Methylation Specific Polymerase Chain Reaction (MSP) for Tumour Suppressor Genes Including RUNX3. Oncology Reports 2007 Nov;18(5):1225-30. 

Lee BTK, Song CM, Yeo BH, Chung CW, Chan YL, Lim TT, Chua YB, Loh MCS, Ang NK, Vijayakumar P, Liew L, Lim J, Lim YP, Wong CH, Chuon D, Rajagopal G and Hill J. Gastric Cancer (Biomarkers) Knowledgebase (GCBKB): A Curated and Fully Integrated Knowledgebase of Putative Biomarkers Related to Gastric Cancer. Biomarker Insights 2007: 2; 135-141. 

2006

Singaraja RR, Van Eck M, Bissada N, Zimetti F, Collins HL, Hildebrand RB, Hayden A, Brunham LR, Kang MH, Fruchart JC, Van Berkel TJ, Parks JS, Staels B, Rothblat GH, Fiévet C, Hayden MR. Both hepatic and extrahepatic ABCA1 have discrete and essential functions in the maintenance of plasma high-density lipoprotein cholesterol levels in vivo. Circulation. 2006;114(12):1301-9.

Singaraja RR, Visscher H, James ER, Chroni A, Coutinho JM, Brunham LR, Kang MH, Zannis VI, Chimini G, Hayden MR. Specific mutations in ABCA1 have discrete effects on ABCA1 function and lipid phenotypes both in vivo and in vitro. Circ Res. 2006;99(4):389-97.

Singaraja RR, Stahmer B, Brundert M, Merkel M, Heeren J, Bissada N, Kang M, Timmins JM, Ramakrishnan R, Parks JS, Hayden MR, Rinninger F. Hepatic ATP-binding cassette transporter A1 is a key molecule in high-density lipoprotein cholesteryl ester metabolism in mice.Arterioscler Thromb Vasc Biol. 2006;26(8):1821-7.

Brunham LR, Singaraja RR, Hayden MR. Variations on a gene: rare and common variants in ABCA1 and their impact on HDL cholesterol levels and atherosclerosis. Annu Rev Nutr. 2006;26:105-129.

Yanai A, Huang K, Kang R, Singaraja RR, Arstikaitis P, Gan L, Orban PC, Mullard A, Cowan CM, Raymond LA, Drisdel RC, Green WN, Ravikumar B, Rubinsztein DC, El-Husseini A, Hayden MR. Palmitoylation of huntingtin by HIP14 is essential for its trafficking and function. Nat Neurosci. 2006 Jun;9(6):824-31. Epub 2006 May 14.

Van Eck M, Singaraja RR, Ye D, Hildebrand RB, James ER, Hayden MR, Van Berkel TJ. Macrophage ATP-binding cassette transporter A1 overexpression inhibits atherosclerotic lesion progression in low-density lipoprotein receptor knockout mice. Arterioscler Thromb Vasc Biol. 2006 Apr;26(4):929-34. Epub 2006 Feb 2.

Brunham LR, Kruit JK, Iqbal J, Fievet C, Timmins JM, Pape TD, Coburn BA, Bissada N, Staels B, Groen AK, Hussain MM, Parks JS, Kuipers F, Hayden MR. Intestinal ABCA1 directly contributes to HDL biogenesis in vivo. J Clin Invest. Apr 2006;116(4):1052-1062.

Brunham LR, Kruit JK, Pape TD, Parks JS, Kuipers F, Hayden MR. Tissue-specific induction of intestinal ABCA1 expression with a liver X receptor agonist raises plasma HDL cholesterol levels. Circ Res. Sep 29 2006;99(7):672-674.

Tan SH, Ida H, Goh BC, Hsieh W, Loh M and Ito Y. Analyses of Promoter Hypermethylation for RUNX3 and other Tumor Suppressor Genes in Nasopharyngeal Carcinoma. Anticancer Research 2006; 26(6); 4287-4292.  

Lau QC, Raja E, Salto-Tellez M, Liu Q, Ito K, Inoue M, Putti TC, Loh M, Ko TK, Huang C, Ito Y and Sukumar S. RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer. Cancer Research 2006 Jul 1;66(13):6512-20. 

2005

Brunham LR, Singaraja RR, Pape TD, Kejariwal A, Thomas PD, Hayden MR. Accurate prediction of the functional significance of single nucleotide polymorphisms and mutations in the ABCA1 gene. PLoS Genet. Dec 2005;1(6):e83.

Singaraja RR, James ER, Crim J, Visscher H, Chatterjee A, Hayden MR. Alternate transcripts expressed in response to diet reflect tissue-specific regulation of ABCA1. J Lipid Res. 2005 Oct;46(10):2061-71. Epub 2005 Jul 16.

Warby SC, Chan EY, Metzler M, Gan L, Singaraja RR, Crocker SF, Robertson HA, Hayden MR. Huntingtin phosphorylation on serine 421 is significantly reduced in the striatum and by polyglutamine expansion in vivo.Hum Mol Genet. 2005 Jun 1;14(11):1569-77. Epub 2005 Apr 20.

Coutinho JM, Singaraja RR, Kang M, Arenillas DJ, Bertram LN, Bissada N, Staels B, Fruchart JC, Fievet C, Joseph-George AM, Wasserman WW, Hayden MR. Complete functional rescue of the ABCA1-/- mouse by human BAC transgenesis. J Lipid Res. 2005 Jun;46(6):1113-23. Epub 2005 Mar 16.

Robbins CS*, Pouladi MA*, Fattouh R, Dawe DE, Vujicic N, Richards CD, Jordana M, Inman MD, Stampfli MR. Mainstream cigarette smoke exposure attenuates airway immune inflammatory responses to surrogate and common environmental allergens in mice, despite evidence of increased systemic sensitization. J Immunol. 2005. 175: 2834-42. (* equal contribution)

Fattouh R, Pouladi MA, Alvarez D, Johnson JR, Walker TD, Goncharova S, Inman MD, Jordana M. House Dust Mite Facilitates Ovalbumin-specific Allergic Sensitization and Airways Inflammation.Am J Respir Crit Care Med. 2005. 172: 314-21.

Mondry A, Liu P, Loh M and Mongelli M. Z-velocity in screening for intrauterine growth restriction. Ultrasound in Obstetrics and Gynecology. 2005 Nov; 26(6):634-8. 

2004

Drannik AG, Pouladi MA, Robbins CS, Goncharova SI, Kianpour S, Stämpfli MR. Impact of Cigarette Smoke on Clearance and Inflammation following Pseudomonas aeruginosa Infection. Am J Respir Crit Care Med. 2004. 170: 1167.

Swirski FK, Gajewska BU, Robbins CS, D'Sa A, Johnson JR, Pouladi MA, Inman MD, Stämpfli MR. Concomitant airway expression of granulocyte-macrophage colony-stimulating factor and decorin, a natural inhibitor of transforming growth factor-beta, breaks established inhalation tolerance. Eur J Immunol. 2004. 34: 2375.

Robbins CS, Dawe DE, Goncharova SI, Pouladi MA, Drannik AG, Swirski FK, Cox G, Stämpfli MR. Cigarette smoke decreases pulmonary dendritic cells and impacts antiviral immune responsiveness. Am J Respir Cell Mol Biol. 2004. 30: 202.

Pouladi MA, Robbins CS, Swirski FK, Cundall M, McKenzie AN, Jordana M, Shapiro SD, Stämpfli MR. Interleukin-13-dependent expression of matrix metalloproteinase-12 is required for the development of airway eosinophilia in mice. Am J Respir Cell Mol Biol. 2004. 30: 84.

Huang K, Yanai A, Kang R, Arstikaitis P, Singaraja RR, Metzler M, Mullard A, Haigh B, Gauthier-Campbell C, Gutekunst CA, Hayden MR, El-Husseini A. Huntingtin-interacting protein HIP14 is a palmitoyl transferase involved in palmitoylation and trafficking of multiple neuronal proteins.Neuron. 2004 Dec 16;44(6):977-86.

Shen Y, Guo Q, Zhukovskaya NL, Drum CL, Bohm A, Tang W-J. Structure of anthrax edema factor-calmodulin-adenosine 5'-(alpha,beta-methylene)-triphosphate complex reveals an alternative mode of ATP binding to the catalytic site. Biochem Biophys Res Commun. 2004 Apr 30;317(2):309–14.

2003

Singaraja RR, Brunham LR, Visscher H, Kastelein JJ, Hayden MR. Efflux and atherosclerosis: the clinical and biochemical impact of variations in the ABCA1 gene. Arterioscler Thromb Vasc Biol. Aug 1 2003;23(8):1322-1332.

Wellington CL, Brunham LR, Zhou S, Singaraja RR, Visscher H, Gelfer A, Ross C, James E, Liu G, Huber MT, Yang YZ, Parks RJ, Groen A, Fruchart-Najib J, Hayden MR. Alterations of plasma lipids in mice via adenoviral-mediated hepatic overexpression of human ABCA1. J Lipid Res. 2003 Aug;44(8):1470-80. Epub 2003 May 1.

2002

Coppolino MG, Dierckman R, Loijens J, Collins RF, Pouladi M, Jongstra-Bilen J, Schreiber AD, Trimble WS, Anderson R, Grinstein S. Inhibition of phosphatidylinositol-4-phosphate 5-kinase Ialpha impairs localized actin remodeling and suppresses phagocytosis. J Biol Chem. 2002. 277: 43849.

Singaraja RR, Fievet C, Castro G, James ER, Hennuyer N, Clee SM, Bissada N, Choy JC, Fruchart JC, McManus BM, Staels B, Hayden MR. Increased ABCA1 activity protects against atherosclerosis. J Clin Invest. 2002;110(1):35-42.

Singaraja RR, Hadano S, Metzler M, Givan S, Wellington CL, Warby S, Yanai A, Gutekunst CA, Leavitt BR, Yi H, Fichter K, Gan L, McCutcheon K, Chopra V, Michel J, Hersch SM, Ikeda JE, Hayden MR. HIP14, a novel ankyrin domain-containing protein, links huntingtin to intracellular trafficking and endocytosis.Hum Mol Genet. 2002 Nov 1;11(23):2815-28.

Attie AD, Hamon Y, Brooks-Wilson AR, Gray-Keller MP, MacDonald ML, Rigot V, Tebon A, Zhang LH, Mulligan JD, Singaraja RR, Bitgood JJ, Cook ME, Kastelein JJ, Chimini G, Hayden MR. Identification and functional analysis of a naturally occurring E89K mutation in the ABCA1 gene of the WHAM chicken. J Lipid Res. 2002 Oct;43(10):1610-7.

See RH, Caday-Malcolm RA, Singaraja RR, Zhou S, Silverston A, Huber MT, Moran J, James ER, Janoo R, Savill JM, Rigot V, Zhang LH, Wang M, Chimini G, Wellington CL, Tafuri SR, Hayden MR. Protein kinase A site-specific phosphorylation regulates ATP-binding cassette A1 (ABCA1)-mediated phospholipid efflux.J Biol Chem. 2002 Nov 1;277(44):41835-42. Epub 2002 Aug 23.

Robitaille J, MacDonald ML, Kaykas A, Sheldahl LC, Zeisler J, Dubé MP, Zhang LH, Singaraja RR, Guernsey DL, Zheng B, Siebert LF, Hoskin-Mott A, Trese MT, Pimstone SN, Shastry BS, Moon RT, Hayden MR, Goldberg YP, Samuels ME. Mutant frizzled-4 disrupts retinal angiogenesis in familial exudative vitreoretinopathy. Nat Genet. 2002 Oct;32(2):326-30. Epub 2002 Aug 12.

Drum CL, Yan S-Z, Bard J, Shen Y-Q, Lu D, Soelaiman S, et al. Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin. Nature. 2002 Jan 24;415(6870):396–402.

2001

Singaraja RR, Bocher V, James ER, Clee SM, Zhang LH, Leavitt BR, Tan B, Brooks-Wilson A, Kwok A, Bissada N, Yang YZ, Liu G, Tafuri SR, Fievet C, Wellington CL, Staels B, Hayden MR. Human ABCA1 BAC transgenic mice show increased high density lipoprotein cholesterol and ApoAI-dependent efflux stimulated by an internal promoter containing liver X receptor response elements in intron 1. J Biol Chem. 2001;276(36):33969-79.

Drum CL, Shen Y, Rice PA, Bohm A, Tang WJ. Crystallization and preliminary X-ray study of the edema factor exotoxin adenylyl cyclase domain from Bacillus anthracis in the presence of its activator, calmodulin. Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1881–4.

2000

Drum CL, Yan SZ, Sarac R, Mabuchi Y, Beckingham K, Bohm A, et al. An extended conformation of calmodulin induces interactions between the structural domains of adenylyl cyclase from Bacillus anthracis to promote catalysis. J Biol Chem. American Society for Biochemistry and Molecular Biology; 2000 Nov 17;275(46):36334–40.

1998

Tang WJ, Yan S, Drum CL. Class III adenylyl cyclases: regulation and underlying mechanisms. Adv Second Messenger Phosphoprotein Res. 1998;32:137–51.

1997

Benzinger GR, Drum CL, Chen LQ, Kallen RG, Hanck DA, Hanck D. Differences in the binding sites of two site-3 sodium channel toxins. Pflugers Arch. 1997 Nov;434(6):742–9.

1996

Kelso GJ, Drum CL, Hanck DA, Blumenthal KM. Role for Pro-13 in directing high-affinity binding of anthopleurin B to the voltage-sensitive sodium channel. Biochemistry. 1996 Nov 12;35(45):14157–64.

Dias-Kadambi BL, Combs KA, Drum CL, Hanck DA, Blumenthal KM. The role of exposed tryptophan residues in the activity of the cardiotonic polypeptide anthopleurin B. J Biol Chem. 1996 Sep 27;271(39):23828–35.

Dias-Kadambi BL, Drum CL, Hanck DA, Blumenthal KM. Leucine 18, a hydrophobic residue essential for high affinity binding of anthopleurin B to the voltage-sensitive sodium channel. J Biol Chem. 1996 Apr 19;271(16):9422–8.

1995

Khera PK, Benzinger GR, Lipkind G, Drum CL, Hanck DA, Blumenthal KM. Multiple cationic residues of anthopleurin B that determine high affinity and channel isoform discrimination. Biochemistry. 1995 Jul 11;34(27):8533–41.