The Drum lab studies new approaches to clinical trials and biomarker discovery, primarily through the use of targeted mass spectroscopy and blood microsampling techniques. Questions such as: how to use ‘natural experiments’ of pharmacogenetic variation to discover adverse and beneficial effects of pharmaceuticals? and how to efficiently assess the predictive value of a novel diagnostic by combining established and prospectively collected patient samples? are reflective of the combined capabilities of the lab, which include clinical protocol development, patient recruitment and materials and technique engineering.
Theme 1: New approaches to clinical trials. The spectrum of materials and methods being developed for disease diagnosis and monitoring is increasing exponentially. A greater efficiency of patient recruitment resources is critically needed to provide an evidence base for clinical use and regulatory approval of these remarkable, new technologies. Working through a multi-institution patient recruitment network, Dr. Drum’s lab combines bespoke clinical protocol development with novel technologies to answer real-world clinical questions of disease prognosis and diagnosis.
Theme 2: Biomarker discovery and validation. Omics technologies have radically altered the information content of sample analysis, measuring hundreds if not thousands of analytes per assay. By virtue of the same multiplex power, the meaning of an individual qualitative marker will often require further validation as a quantified, validated assay. Quantitative mass spectroscopy represents a rapidly adaptable, highly quantitative method for rapid hypothesis testing in clinical samples. Results can be interpreted in the context of the literature and patient characteristics, derivative hypothesis made and iterated against the same samples without the time intensive need of developing new affinity based assays.
Dr. Chester Drum is a faculty at the Translational Laboratories in Genetic Medicine at the Agency for Science, Technology and Research (A*STAR), and an Assistant Professor at the National University of Singapore, Yong Loo Lin School of Medicine. He is a member of the Cardiovascular Research Institute, a consultant cardiologist at National University Hospital and is board certified in both the United States and Singapore.
Mylene Pestano (NUH)
Kay Mhine (NUH)
Jonna Ernie (NUH)
Wai Kong Thang (NUH)
Vimala Sadaiyappan (NHC)
Katerina Oh (NHC)
Warren Tan (NGS)
Nihar Mazurkar (YLLSoM)
Garcia-Miralles M, Ooi J, Ferrari Bardile C, Tan LJ, George M, Drum CL, Lin RY, Hayden MR, Pouladi MA. Treatment with the MAO-A inhibitor clorgyline elevates monoamine neurotransmitter levels and improves affective phenotypes in a mouse model of Huntington disease. Exp Neurol. 2016 Apr;278:4-10. doi: 10.1016/j.expneurol.2016.01.019.
Halliwell B, Cheah IK, Drum CL. Ergothioneine, an adaptive antioxidant for the protection of injured tissues? A hypothesis.Biochem Biophys Res Commun. 2016 Feb 5;470(2):245-50. doi: 10.1016/j.bbrc.2015.12.124.
Patra A, Ding T, Engudar G, Wang Y, Dykas MM, Liedberg B, Kah JC, Venkatesan T, Drum CL. Component-Specific Analysis of Plasma Protein Corona Formation on Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance. Small. 2016 Mar 2;12(9):1174-82. doi: 10.1002/smll.201501603.
Patra A, Ding T, Engudar G, Yi W, Dykas M, Liedberg B, Kah J, Venkatesan T, Drum CL. Component Specific Analysis of Plasma Protein Corona Formation On Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance. Small. 2015 (selected for early online publication, Sept 10, 2015): doi: 10.1002/smll.201501603
Ding T, Hong M, Richards AM, It Wong Ten, Zhou X, Drum CL. Quantification of a Cardiac Biomarker in Human Serum Using Extraordinary Optical Transmission (EOT). PLoS ONE. 2015 Mar 16;10(3):e0120974.
Drum CL. System-level prescriptions for adaptive drug licensing. Science Translational Medicine. 2015 Mar 4;7(277):277ec36.
Chan JM, Wong KHK, Richards AM, Drum CL. Microengineering in cardiovascular research: new developments and translational applications. cardiovascular research. 2015 Feb 17. (editor’s choice)
Drum CL. TRIPOD puts prediction models on a firmer footing. Science Translational Medicine. 2015 Jan 28;7(272):272ec14–4.
Drum CL. Watch your leftovers: Remnant cholesterol brings oversized risk to cardiovascular health. Science Translational Medicine. 2014 Dec 3;6(265):205.
Drum CL. Short circuiting mitochondria leads to improvement in mouse models of diabetes. Science Translational Medicine. 2014 Oct 22;6(259):179.
Drum CL. The ubiquitinase pathway takes center stage in viral myocarditis. Science Translational Medicine. 2014 Sep 10;6(253):155.
Yu J, Tao D, Ng EX, Drum CL, Liu AQ, Chen C-H. Real-time measurement of thrombin generation using continuous droplet microfluidics. Biomicrofluidics. 2014 Sep;8(5):052108.
Drum CL. Long non-coding RNAs in myocardial infarction. Science Translational Medicine. 2014 Jul 30;6(247):131.
Drum CL. Taking Cancer Cells Out of Circulation. Science Translational Medicine. 2014 Jun 18;6(241):104.
Drum CL. Magnetic Resonance: It’s a Gas! Science Translational Medicine. 2014 May 7;6(235):80.
Drum CL. Getting to the Heart of Consciousness. Science Translational Medicine. 2014 Mar 26;6(229):53.
Eichler H-G, Oye K, Baird LG, Abadie E, Brown J, Drum CL, et al. Adaptive licensing: taking the next step in the evolution of drug approval. Clin Pharmacol Ther. 2012 Mar;91(3):426–37.
De Sousa FB, Lima AC, Denadai AML, Anconi CPA, De Almeida WB, Novato WTG, et al. Superstructure based on β-CD self-assembly induced by a small guest molecule. Phys Chem Chem Phys. The Royal Society of Chemistry; 2012 Feb 14;14(6):1934–44.
Chan JM, Rhee J-W, Drum CL, Bronson RT, Golomb G, Langer R, et al. In vivo prevention of arterial restenosis with paclitaxel-encapsulated targeted lipid-polymeric nanoparticles. Proc Natl Acad Sci USA. National Acad Sciences; 2011 Nov 29;108(48):19347–52.
Shen Y, Guo Q, Zhukovskaya NL, Drum CL, Bohm A, Tang W-J. Structure of anthrax edema factor-calmodulin-adenosine 5'-(alpha,beta-methylene)-triphosphate complex reveals an alternative mode of ATP binding to the catalytic site. Biochem Biophys Res Commun. 2004 Apr 30;317(2):309–14.
Drum CL, Yan S-Z, Bard J, Shen Y-Q, Lu D, Soelaiman S, et al. Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin. Nature. 2002 Jan 24;415(6870):396–402.
Drum CL, Shen Y, Rice PA, Bohm A, Tang WJ. Crystallization and preliminary X-ray study of the edema factor exotoxin adenylyl cyclase domain from Bacillus anthracis in the presence of its activator, calmodulin. Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1881–4.
Drum CL, Yan SZ, Sarac R, Mabuchi Y, Beckingham K, Bohm A, et al. An extended conformation of calmodulin induces interactions between the structural domains of adenylyl cyclase from Bacillus anthracis to promote catalysis. J Biol Chem. American Society for Biochemistry and Molecular Biology; 2000 Nov 17;275(46):36334–40.
Tang WJ, Yan S, Drum CL. Class III adenylyl cyclases: regulation and underlying mechanisms. Adv Second Messenger Phosphoprotein Res. 1998;32:137–51.
Benzinger GR, Drum CL, Chen LQ, Kallen RG, Hanck DA, Hanck D. Differences in the binding sites of two site-3 sodium channel toxins. Pflugers Arch. 1997 Nov;434(6):742–9.
Kelso GJ, Drum CL, Hanck DA, Blumenthal KM. Role for Pro-13 in directing high-affinity binding of anthopleurin B to the voltage-sensitive sodium channel. Biochemistry. 1996 Nov 12;35(45):14157–64.
Dias-Kadambi BL, Combs KA, Drum CL, Hanck DA, Blumenthal KM. The role of exposed tryptophan residues in the activity of the cardiotonic polypeptide anthopleurin B. J Biol Chem. 1996 Sep 27;271(39):23828–35.
Dias-Kadambi BL, Drum CL, Hanck DA, Blumenthal KM. Leucine 18, a hydrophobic residue essential for high affinity binding of anthopleurin B to the voltage-sensitive sodium channel. J Biol Chem. 1996 Apr 19;271(16):9422–8.
Khera PK, Benzinger GR, Lipkind G, Drum CL, Hanck DA, Blumenthal KM. Multiple cationic residues of anthopleurin B that determine high affinity and channel isoform discrimination. Biochemistry. 1995 Jul 11;34(27):8533–41.
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