John E CONNOLLY

Immuno-oncology & Cell Therapy
PhD - Immunology, Dartmouth Medical School, UK
PhD - Immunology, Dartmouth Medical School, UK
SUMMARY
Dr John E. Connolly is a Research Director for Translational Immunology at A*STAR Institute of Molecular and Cell Biology (A*STAR IMCB).
John joined A*STAR Singapore Immunology Network (A*STAR SIgN) in 2010, where he founded and ran the immunomonitoring platform, before moving to A*STAR IMCB in 2013. John’s group at A*STAR IMCB explores broad topics in translational immunology, including human studies in dendritic cell biology, immunometabolism, primary immunodeficiency, and immunomonitoring of clinical infectious disease cohorts. From 2011, he has been a Director of ASPIRE, the A*STAR Programme in Translational Research on Infectious Disease. He has also held concurrent appointments as Chief Scientific Officer at Tessa Therapeutics in Singapore from 2017 to 2020, and now as Chief Scientific Officer at the Parker Institute for Cancer Immunotherapy in San Francisco, CA, USA.
John received his PhD in Immunology from Dartmouth Medical School and studied human dendritic cell biology under Dr Michael Fanger. During this time he was involved in the development of immunotherapeutic preclinical models and clinical trials for Glioblastoma multiforme (GBM). He moved to the Baylor Institute for Immunology Research, a fully translational research institute dedicated to rationally designed vaccines against cancer and infectious disease. John served as the Director of Research Initiatives for the Baylor Research Institute, leading a large integrated translational research resource and multi-institutional programs that involved a number of international sites. During his tenure at Baylor, John was a central core facility director of the NIAID Centers for Translational Research on Human Immunology and Biodefense, an NIH funded consortium of basic, translational research and clinical trials focused on vaccine design. John is the past President of the Board of Directors of The American Cancer Society in N. Texas.
As a human immunologist, John’s research interests focus on target discovery for immune modulation.
- 2024: RIE2025 Human Health and Potential (HHP) Industry Alignment Fund Pre-Positioning (IAF-PP) (Theme PI)
- 2024: A*STAR I&E Gap Funding (PI)
- 2023: RIE2025 Human Health and Potential (HHP) Industry Alignment Fund Pre-Positioning (IAF-PP) (Co-I)
- 2023: Ministry of Health Open Fund Individual Research Grant (OF-IRG) (Co-I)
- 2022: Research Collaboration with Industry (PI)
- 2021: Research Collaboration with Industry (PI)
- 2020: National Medical Research Council (NMRC) Open Fund Large Collaborative Grant (OF-LCG) (Theme PI)
- 2020: Research Collaboration with Industry (PI)
- 2019: Industry Alignment Fund Pre-Positioning (IAF-PP) (Theme PI)
- 2018: National Medical Research Council (NMRC) Open Fund Large Collaborative Grant (OF-LCG) (Theme PI)
RESEARCH
My background is in human immunology, and the primary research focus of my lab is studying early events in antigen presenting cell biology which dictate the balance between immunity and tolerance. This research has led to the development of quantifiable improvements in clinical care including INDs in immune oncology, a primary immune deficiency service and two biotech spin-off companies (Tessa Therapeutics and Twain Therapeutics).
PUBLICATIONS
- Unanswered questions following reports of secondary malignancies after CAR-T cell therapy.
Bruce L. Levine, Marcelo C. Pasquini, John E Connolly, David L. Porter, Michael P. Gustafson, Jaap J. Boelens, Edwin M. Horwitz, Stephan A. Grupp, Marcela V. Maus, Frederick L. Locke, Fabio Ciceri, Annalisa Ruggeri, John Snowden, Helen E. Heslop, Crystal L. Mackall, Carl H. June, Anna M. Sureda & Miguel-Angel Perales (2024)
Nature Medicine, 9 Jan 2024 volume 30, pages338–341
- A novel GPI-anchored dominant-negative TGF-β receptor II renders T cells unresponsive to TGF-β signalling.
Sven H Petersen, Kays Al Badawy, 1Richard Hopkins, Dang L Vu, Mehran Rahmani , Sonia M P Maia, John E Connolly (2023)
Mol Ther Oncolytics, 2023 Sep 25:31:100730, doi: 10.1016/j.omto.2023.100730, PMID: 37829123 - Natural killer cell memory precedes HLH in monozygotic twins discordant for chronic active Epstein-Barr virus disease.
Chrissie K Lim, Youjia Zhong , Richard Hopkins, Wei-Xiang Sin, Bijin Veonice Au, Sriram Narayanan, Chiung-Hui Huang, Colin Y C Lee, Ming Liang Oon, Avisha Chowdhury , Benjamin Wong, Frances Yeap, Mariflor Villegas, Julien Pompon, Patricia P L Ng, Siok-Bian Ng , Thuan Chong Quah, Poh-Lin Tan, Keh-Chuang Chin, John E Connolly (2023)
Blood, 2023 Apr 27;141(17):2151-2155. doi: 10.1182/blood.2022018309, PMID: 36877914, DOI: 10.1182/blood.2022018309
- Dendritic cell therapy with CD137L-DC-EBV-VAX in locally recurrent or metastatic nasopharyngeal carcinoma is safe and confers clinical benefit.
Emily Nickles, Bhushan Dharmadhikari, Li Yating, Robert J Walsh, Liang Piu Koh, Michelle Poon, Lip Kun Tan, Ling-Zhi Wang, Yvonne Ang, Yugarajah Asokumaran, Wan Qin Chong, Yiqing Huang, Kwok Seng Loh, Joshua Tay, Ross Soo, Mickey Koh, Liam Pock Ho, Marieta Chan, Madelaine Niam, Melissa Soh, Yen Hoon Luah, Chwee Ming Lim, Nivashini Kaliaperumal, Veonice B Au, Najwa Binte Said Nasir Talib, Reina Sng, John E Connolly, Boon Cher Goh, Herbert Schwarz (2022)
Cancer Immunol Immunother, 2022 Jun;71(6):1531-1543. doi: 10.1007/s00262-021-03075-3. Epub 2021 Oct 18, PMID: 34661709, PMCID: PMC10991364, DOI: 10.1007/s00262-021-03075-3
- Effective Killing of Acute Myeloid Leukemia by TIM-3 Targeted Chimeric Antigen Receptor T Cells.
Wen-Hsin Sandy Lee, Zhiyong Ye, Alice M.S. Cheung, Y.P. Sharon Goh, Hsueh Ling Janice Oh, Ravisankar Rajarethinam, Siok Ping Yeo, Mun Kuen Soh, Esther Hian Li Chan, Lip Kun Tan, Soo-Yong Tan, Charles Chuah, Wee Joo Chng, John E Connolly and Cheng-I Wang (2021)
Mol Cancer Ther, 2021 Sep;20(9):1702-1712. doi: 10.1158/1535-7163.MCT-20-0155. Epub 2021 Jun 22. PMID: 34158344, DOI: 10.1158/1535-7163.MCT-20-0155 - Monocytic Myeloid-Derived Suppressor Cells Underpin Resistance to Adoptive T Cell Therapy in Nasopharyngeal Carcinoma.
Richard Hopkins, Wenwei Xiang, Damien Marlier, Veonice Bijin Au, Qianting Ching, Lynn Xue Wu, Rujun Guan, Bernett Lee, Whay-Kuang Chia, Who-Whong Wang, Joseph Wee, Joanna Ng, Rachael Cheong, Shuting Han, Axel Chu, Chit Lai Chee, Timothy Shuen, Michael Podinger, Alexander Lezhava, Han Chong Toh, John E Connolly (2021)
Mol Ther, 2021 Feb 3;29(2):734-743. doi: 10.1016/j.ymthe.2020.09.040. Epub 2020 Sep 30. PMID: 33038324, PMCID: PMC7854281, DOI: 10.1016/j.ymthe.2020.09.040 - Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.
Patricia J. Ahl, Richard A. Hopkins, Wen Wei Xiang, Bijin Au, Nivashini Kaliaperumal, Anna-Marie Fairhurst, John E. Connolly (2020)
Communications Biology volume 3, Article number: 305 (2020) - A Dominant-Negative NFKBIA Mutation Promotes IL-1β Production Causing Hepatic Disease with Severe Immunodeficiency.
Enrica Ek Tan, Richard A Hopkins, Chrissie K Lim, Saumya S Jamuar, Christina Ong, Koh C Thoon , Mark Ja Koh , Eun Mong Shin, Derrick Wq Lian, Madhushanee Weerasooriya, Christopher Zw Lee, Andreas Alvin Pumomo Soetedjo , Chang Siang Lim, Veonice B Au, Edmond Chua, Hui Yin Lee, Leigh Ann Jones, Sharmy S James, Nivashini Kaliaperumal, Jeffery Kwok, Ee Shien Tan, Biju Thomas, Lynn Xue Wu, Lena Ho, Anna Marie Fairhurst, Florent Ginhoux, Adrian Kk Teo, Yong Liang Zhang, Kok Huar Ong, Weimiao Yu, Byrappa Venkatesh, Vinay Tergaonkar, Bruno Reversade, Keh Chuang Chin , Ah Moy Tan, Woei Kang Liew , John E Connolly (2020)
J Clin Invest, 2020 Nov 2;130(11):5817-5832, doi: 10.1172/JCI98882, PMID: 32750042, PMCID: PMC7598087, DOI: 10.1172/JCI98882
- TLR7 Protein Expression in Mild and Severe Lupus-Prone Models Is Regulated in a Leukocyte, Genetic, and IRAK4 Dependent Manner.
Teja Celhar, Hao Kim Lu, Lia Benso, Larissa Rakhilina, Hui Yin Lee, Shubhita Tripathi, Olga Zharkova, Wei Yee Ong, Hiroko Yasuga, Bijin Au, Damien Marlier, Lina Hsiu Kim Lim, Thomas Paulraj Thamboo, John S. Mudgett, Matthew F. Mackey, Dennis M. Zaller, John E Connolly, Anna-Marie Fairhurst (2019)
Front Immunol 2019 Jul 10:10:1546. doi: 10.3389/fimmu.2019.01546. eCollection 2019. PMID: 31354711, PMCID: PMC6636428, DOI: 10.3389/fimmu.2019.01546 - Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine.
David A. G. Skibinski, Leigh Ann Jones, Yuan O. Zhu, Lin Wu Xue, Bijin Au, Bernett Lee, Ahmad Nazri Mohamed Naim, Audrey Lee, Nivashini Kaliaperumal, Jenny G. H. Low, Lawrence S. Lee, Michael Poidinger, Philippe Saudan, Martin Bachmann, Eng Eong Ooi, Brendon J. Hanson, Veronica Novotny-Diermayr, Alex Matter, Anna-Marie Fairhurst, Martin L. Hibberd, John E. Connolly (2018)
Scientific Reports volume 8, Article number: 18007 (2018)
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