Radoslaw SOBOTA

Dr. rer. nat. Life Sciences (PhD), RWTH-Aachen, Germany
SUMMARY
- 2019: National Research Foundation (NRF) Grant (Role: Co-PI)
- 2015: National Medical Research Council (NMRC) Grant – MS-CETSA platform (Role: Co-I)
- 2015: A*STAR Biomedical Research Council (BMRC) Young Investigator Grant
RESEARCH
My long-term research interest is focused on the integration of the cell biology, immunology with quantitative mass spectrometry-based proteomics. It includes multiomics approaches in precision medicine for personalised therapies, gene therapies, antigen identification pipelines development, body fluid biomarker discovery, clinical proteomics, novel drug target deconvolution using MS-CETSA and chemical proteomics for patient-tailored therapies based on spatial-multiomics and single cell proteomics data integration. Overall, I have contributed to more than 80 publications with more than 4,300 citations (H index =33) on Google Scholar.
PUBLICATIONS
- Spatial omics techniques and data analysis for cancer immunotherapy applications.
Zhang Y, Lee RY, Tan CW, Guo X, Yim WW, Lim JC, Wee FY, Yang WU, Kharbanda M, Lee JJ, Ngo NT, Leow WQ, Loo LH, Lim TK, Sobota RM*, Lau MC, Davis MJ, Yeong J. (2024)
Curr Opin Biotechnol. Jun;87:103111. doi: 10.1016/j.copbio.2024.103111. Epub 2024 Mar 22. PMID: 38520821.
*co-senior author
- Engineering cell-free systems by chemoproteomic-assisted phenotypic screening.
Levitskaya Z, Ser Z, Koh H, Mei WS, Chee S, Sobota RM, Ghadessy JF. (2024)
RSC Chem Biol. 2024 Mar 6;5(4):372-385. doi: 10.1039/d4cb00004h. PMID: 38576719; PMCID: PMC10989505.
*co-corresponding author - Unveiling the influence of tumor microenvironment and spatial heterogeneity on temozolomide resistance in glioblastoma using an advanced human in vitro model of the blood-brain barrier and glioblastoma.
Lam MS, Aw JJ, Tan D, Vijayakumar R, Lim HYG, Yada S, Pang QY, Barker N, Tang C, Ang BT, Sobota RM, Pavesi A.(2023)
Small. 2023 Dec;19(52):e2302280. doi: 10.1002/smll.202302280. Epub 2023 Aug 30. PMID: 37649234.
- iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer.
Park DS, Kozaki T, Tiwari SK, Moreira M, Khalilnezhad A, Torta F, Olivié N, Thiam CH, Liani O, Silvin A, Phoo WW, Gao L, Triebl A, Tham WK, Gonçalves L, Kong WT, Raman S, Zhang XM, Dunsmore G, Dutertre CA, Lee S, Ong JM, Balachander A, Khalilnezhad S, Lum J, Duan K, Lim ZM, Tan L, Low I, Utami KH, Yeo XY, Di Tommaso S, Dupuy JW, Varga B, Karadottir RT, Madathummal MC, Bonne I, Malleret B, Binte ZY, Wei Da N, Tan Y, Wong WJ, Zhang J, Chen J, Sobota RM, Howland SW, Ng LG, Saltel F, Castel D, Grill J, Minard V, Albani S, Chan JKY, Thion MS, Jung SY, Wenk MR, Pouladi MA, Pasqualini C, Angeli V, Cexus ONF, Ginhoux F. (2022)
Nature. 2023 Nov;623(7986):397-405. doi: 10.1038/s41586-023-06713-1. Epub 2023 Nov 1. PMID: 37914940.
- Hybrid structural modeling of alloantibody binding to human leukocyte antigen with rapid and reproducible cross-linking mass spectrometry.
Ser Z, Gu Y, Yap J, Lim YT, Wang SM, Hamidinia M, Murali TM, Kumar R, Gascoigne NR, MacAry PA, Sobota RM. (2023)
Cell Rep Methods. 2023 Sep 25;3(9):100569. doi: 10.1016/j.crmeth.2023.100569. Epub 2023 Aug 30. PMID: 37751693; PMCID: PMC10545907. - Complete sequences of the velvet worm slime proteins reveal that slime formation is enabled by disulfide bonds and intrinsically disordered regions.
Lu Y, Sharma B, Soon WL, Shi X, Zhao T, Lim YT, Sobota RM, Hoon S, Pilloni G, Usadi A, Pervushin K, Miserez A. (2022)
Adv Sci (Weinh). 2022 Jun;9(18):e2201444. doi: 10.1002/advs.202201444. Epub 2022 May 18. PMID: 35585665; PMCID: PMC9218773. - Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome.
Dziekan JM, Wirjanata G, Dai L, Go KD, Yu H, Lim YT, Chen L, Wang LC, Puspita B, Prabhu N, Sobota RM*, Nordlund P, Bozdech Z. (2020)
Nat Protoc. 2020 Jun;15(6):1881-1921. doi: 10.1038/s41596-020-0310-z. Epub 2020 Apr 27. PMID: 32341577.
*co-corresponding author
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