Flow Cytometry

PI/Head: Anis LARBI, Ph.D
Email: Anis_Larbi@immunol.a-star.edu.sg

Manager: Ivy LOW, CCy
Email: Ivy_Low@immunol.a-star.edu.sg

Flow Cytometry Services
Operators: Nurul A’in Binte Mohamed Kassim
Email: bmsiflowcyto@immunol.a-star.edu.sg
Booking: https://au.ppms.info/sign-astar/start/

For more information regarding the SIgN Flow Cytometry Platform and services, please download a copy of our brochure here.

The SIgN Flow Cytometry Platform is an established, state-of-the-art facility that uses cutting-edge cell sorters and analyzers to identify up to 28 parameters for a single cell culture

Background

Flow cytometry is a popular technique that is applied across many fields of biological research, thanks to its wide range of applications in clinical and research laboratories. Using this technique, various cellular properties can be readily identified by measuring the fluorescence levels of the fluorophore-labelled cells at varying wavelengths. These properties include the granularity and size of the cells, their fluorescent intensity and their cellular expression levels of a protein of interest.  A huge advantage of flow cytometry is that it permits the analysis of a large number of cells within a short period of time, allowing for samples to be easily identified, quantified and incorporated into immunophenotyping pipelines. 

Technologies and Approach

Our Flow Cytometry Platform is one of the largest in South-East Asia, with dedicated technical support staff available to provide guidance at each experimental stage, from initial study design and staining protocols, to cell sorting and data interpretation. Specifically, the Flow Cytometry Platform can achieve high-throughput analyses with its available five lasers, and deep cellular phenotyping using up to 30 different colours (Figure 1).  Our facility personnel undergo ICCE certification (International Society for Advancement of Cytometry). For more information on how the Flow Cytometry team is dedicated to improve the quality of data, please follow this link: http://www.research.a-star.edu.sg/research/7612/new-tool-to-clean-flow-cytometry-data.


Figure 1: Cell populations clustered according to phenotypic similarity using the tSNE platform. 

Whole blood stained with 25 surface markers to discriminate 38 different cellular clusters. Data obtained at SIgN using the FACS Symphony.

Regulations: Training sessions are provided to new and less-experienced users to permit independent operation of the analyzers.  All staff undergo ICCE certification (International Society for Advancement of Cytometry). Researchers must wear lab coats, gloves and covered shoes at all times. No food or drinks are allowed in the facility.  All samples must be disposed of into the provided biohazard bags after use. Only facility staff members are allowed to use the Aria II and BD Influx.  It is the responsibility of the researchers to clean the machines after every use according to the stated standard operating procedure. The sheath tank must be filled with 1X PBS and waste must be disposed of and decontaminated in 10% bleach solution.  The last user must switch off the machine and computer.