Mass Cytometry (CyTOF^®) Platform

PI/Head: Olaf ROTZSCHKE, Ph.D.
Email: SIgN_IMP@Immunol.a-star.edu.sg

Mass cytometry instruments represent an alternative technology to flow cytometry, utilizing heavy metals conjugated to antibodies instead of fluorescent molecules for cell phenotyping with minimal spillover. With ongoing advancements in mass cytometry technology, up to 50 antibodies can now be interrogated on millions of individual cells. At SIgN, two Helios^TM Mass Cytometers and a Hyperion^TM Imager are available, which enable immunoprofiling of suspension cells and tissues. We enable comprehensive immunophenotyping, immuno-monitoring, and specific cellular analyses of immune responses.

Helios- Suspension Mass Cytometry

Our predefined Suspension Mass Cytometry panels are not limited to:

• Immunophenotyping & Exploration of Novel Cell Populations
• Discovery of Antigenic CD8+ T cells with our customized MHC-I Tetramer panel
• Disease orientated such as T cell oncology, COVID and Allergy

For more detailed information, please refer HERE.

If your targets of interest are not covered by the SIgN predefined panels or if you would like to integrate specific probes other than antibodies, we can work with you to customize a panel that suits your specific research needs.

For more details, contact SIgN_IMP@Immunol.a-star.edu.sg

Hyperion- Imaging Mass Cytometry

When used alongside Helios^TM, the Hyperion^TM Imaging System uses a one-step staining and detection approach that enables samples to be simultaneously stained, acquired, and analyzed. Standardized Panel kits are available HERE.

Feel free to reach out to us if you have any enquiries about customizing your panel.

Selected Publications

1. Lee, W.W.L., et al. (2024). Counterproductive effects of anti-CD38 and checkpoint inhibitor for the treatment of NK/T cell lymphoma. Frontiers in Immunology, 15, 2024. doi: 10.3389/fimmu.2024.1346178
2. Lee, W.W.L, et al. (2023). Eosinophilic allergic rhinitis is strongly associated with the CD45RBlo subset of CD161+ Th2 cells that secretes IL-2, IL-3, IL-4, IL-5, IL-9, and IL-13. Allergy, 2023, August 7. doi: 10.1111/all.15846
3. Lim, J., et al. (2021). Data-driven analysis of COVID-19 reveals persistent immune abnormalities in convalescent severe individuals. Frontiers in Immunology, 12, 710217. doi: 10.3389/fimmu.2021.710217
4. Chan, Y.H., et al. (2021). Asymptomatic COVID-19: disease tolerance with efficient anti-viral immunity against SARS-CoV-2. EMBO Molecular Medicine, 13(6), e14045. doi: 10.15252/emmm.202114045
5. Lim, J. (2020). Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysis. Scientific Reports, 10(1), 14897. doi: 10.1038/s41598-020-71785-2
6. Newell, E.W. et al. (2009) Simultaneous detection of many T-cell specificities using combinatorial tetramer staining. Nature Methods, 6(7): 497-499. doi: 10.1038/nmeth.1344