Immunogenomics

Head: Shanshan HOWLAND, Ph.D.

Manager: Josephine LUM

The Immunogenomics Platform facilitates in-house sequencing, single-cell analyses and microbiome interrogations using the latest functional genomics technologies

Overview

The SIgN Immunogenomics Platform supports all aspects of genomics research with state-of-the-art technology and high-level technical expertise. We provide research consultations and engage with SIgN scientists to ensure that they reach their experimental goals using the latest, emerging functional genomics technologies. We apply stringent quality control measures throughout the work flow to ensure accurate, reliable and reproducible results.

Technologies and Approach

Our technologies include Affymetrix and Illumina platforms for one-color microarray technology and Next Generation Sequencing technologies using the Applied Biosystems SOLiD 3 System, Illumina Genome Analyzer IIx and Illumina HiSeq 2000 platforms (Figure 1). These technologies allow us to sequence genomes and metagenomes and study transcriptomes, epigenomes and regulomes.

Applications of our technologies include:

•    Gene expression profiling
•    Gene Expression in FFPE samples
•    Exon-specific expression analysis
•    Transcript isoform discovery
•    Small RNA expression profiling
•    Small RNA discovery
•    Chromatin Immuno-precipitation studies
•    Methylation and epigenetic studies
•    Genetic variation detection


Figure 1: Equipment available in the SIgN Immunogenomics laboratory.