Home >Publications

 57.

 Yeo W L, Heng E, Tan L L, Lim Y W, Lim Y H, Hoon S, Zhao H, Zhang M M, Wong F T*. Characterization of Cas Proteins for CRISPR-Cas Editing in Streptomycetes. bioRxiv Preprint 2019 Jan. doi: https://doi.org/10.1101/526996

 56. Gao X, Ran N, Dong X, Zuo B, Yang R, Zhou Q, Moulton H M, Seow Y, Yin H. Anchor Peptide Captures, Targets and Loads Exosomes of diverse Origins for Diagnostics and Therapy Sci. Transl. Med. 2018 Jun. doi: 10.1126/scitranslmed.aat0195 
 55. Alfatah M, Wong J H, Nge C E, Kong K W, Low K N, Leong C Y, Crasta S, Munusamy M, Chang A M L, Hoon S, Ng S B, Kanagasundaram Y, Arumugam P. Hypoculoside, A Sphingoid Base-like Compound From Acremonium Disrupts The Membrane Integrity of Yeast Cells Sci. Rep. 2019 Jan. doi: 10.1038/s41598-018-35979-z 
 54. Goh W L, Lee M Y, Lim T X, Chua J S, Brenner S, Ghadessy F J, Teo Y N. A Novel Molecular Rotor Facilitates Detection of p53-DNA Interactions Using The Fluorescent Intercalator Displacement Assay Sci. Rep. 2018 Aug. doi: 10.1038/s41598-018-31197-9  
 53. Makhija H, Roy S, Hoon S, Ghadessy F J, Wong D, Jaiswal R, Campana D, Droge P. A Novel lamda Integrase-Mediated Seamless Vector Transgenesis Platform for Therapeutic Protein Expression  Nucleic Acids Res. 2018 Sep. doi: 10.1093/nar/gky500. 
 52. Chin S Y*, Poh Y C, Kohler A-C, Sia S K.   An Additive Manufacturing Technique for The Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components JOVE 2018 Jul. doi: 10.3791/56727  (*corresponding author)

 51. Lim Y H, Wong F T*, Yeo W L, Ching K C, Lim Y W, Heng E, Chen S, Tsai D-J, Lauderdale T-L, Shia K-S, Ho Y S, Hoon S, Ang E L, Zhang M.  Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation Chem BioChem. 2018 May. doi: 10.1002/cbic.201800266.  (*co-first-author)
 50. Li H*, Goh BN, Teh WK, Jiang Z, Goh JPZ, Goh A, Wu G, Hoon SS, Raida M, Camattari A, Yang L, O'Donoghue AJ, Dawson TL Jr. J.  Skin Commensal Malassezia globosa Secreted Protease Attenuates Staphylococcusaureus Biofilm Formation J. Invest. Dermatol. 2018 May. doi: 10.1016/j.jid.2017.11.034.  (*corresponding author)
 49.  Ng S. M. S., Yap J. M., Lau Q. Y., Ng F. M., Ong E. H. Q., Barkham T., Teo J. W. P., Alftah M., Kong K. W.Hoon S., Arumugam P. Hill J., Chia C. S. B. Structure-activity Relationship Studies of Ultra-short Peptides with Potent Activities against Fluconazole-resistant Candida albicans Eur. J. Med. Chem. 2018 Mar. doi:10.1016/j.ejmech. 2018.03.027  
 48.  Lim P. Y., Tan L. L., Ow D. S. W., Wong F. T. A Propeptide Toolbox for Secretion Optimization of Flavobcterium Meningosepticum Endopeptidase in Lactococcus Lactis Microbial Cell Factories 2017 Dec. doi: 10.1186/s12934-017-0836-0  
 47. Amini S., Kolle S., Petrone L., Ahanotu O., Sunny S., Sutanto C. N., Hoon S., Cohen L., Weaver J. C., Aizenberg J., Vogel N., Miserez A. Preventing Mussel Adhesion Using Lubricant-Infused Materials Science 2017 Aug. doi: 10.1126/science.aai8977
 46. Yeo C. R., Agrawal M., Hoon S., Shabbir A., Shrivastava M. K., Huang S., Khoo C. M., Chhay V., Yassin M. S., Tai E. S., Vidal-Puig A., Toh S. A. SGBS Cells As A Model of Human Adipocyte Browning: A Comprehensive Comparative Study with Primary Human White Subcutaneous Adipocytes Sci. Rep. 2017 Jun. doi: 10.1038/s41598-017-04369-2.
 45. Cutis H. J., Seow Y., Wood M. J. A., Varela M. A. Knockdown and Replacement Therapy Mediated by Artificial Mirtrons in Spinocerebellar Ataxia 7. Nucleic Acids Res. 2017 May. doi: 10.1093/nar/gkx483
 44.  Zhang M. M., Wong F. T.*, Wang, Y., Luo S., Lim Y. H., Heng E., Yeo W. L., Cobb R. E., Enghiad B., Ang E. L., Zhao H. CRISPR-Cas9 Strategy for Activation of Silent Streptomyces Biosynthetic Gene Clusters Nature Chemical Biology 2017 Apr. DOI:10.1038/nchembio.2341 (*co-first-author)
 43.  Nobusawa K., Okamota N., Chong K. S. L., Lin X., Iwahori K., Yamashita I. Dispersed Gold Nanoparticle Array Produced by Apoferritins Utilizing Biomineralization and Chemical Conversion ACS OMEGA 2017 Apr. DOI: 10.1021/acsomega.6b00550
 42.  Loke J. J., Kumar A., Hoon S., Verma C., Miserez A. Hierachical Assembly of Tough Bioelastomeric Egg Capsules is Mediated by a Budling Protein. Biomacromolecules 2017 Mar. DOI: 10.1021/acs.biomac.6b01810
 41.  Pek H. B., Lim P. Y., Liu C., Lee D. Y., Bi X., Wong F. T.*, Ow D. S. Cytoplasmic Expression of a Thermostable invertase from Thermotoga Maritima in Lactococcus Lactis. Biotechnol Lett. 2017 Feb. DOI: 10.1007/s10529-017-2295-4 (*co-corresponding-author)
 40.  Chin S. Y.*, Poh Y. C., Kohler, A-C., Compton J. T., Hsu L. L. Lau K. M., Kim S., Lee B. W., Lee F. Y., Sia S. K. Additive Manufacturing of Hydrogel-based Materials for Next-generation Implantable Medical Devices. Science Robotic 2017 Jan. DOI: 10.1126/scirobotics.aah6451(*corresponding author)
 39. Tan L. L., Hoon S., Wong F. T. Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLoS One 11(10):e0165074. doi:10.1371/journal.pone.0165074
 38.   Li H., O’Donoghue AJ, van der Linden WA, Xie SC, Yoo E, Foe IT, Tilley L, Craik CS, da Fonseca PCA and Bogyo  Structure and function based design of Plasmodium-selective proteasome inhibitors.  Nature 2016 530:233-236.  
 37.  Han G, Gu B, Cao L, Gao X, Wang Q, Seow Y, Zhang N, Wood MJ, Yin H. Hexose enhances oligonucleotide delivery and exon skipping in dystrophin-deficient mdx mice. Nat Commun. 2016 Mar 11;7:10981. doi: 10.1038/ncomms10981. 
 36.  Cao L, Han G, Lin C, Gu B, Gao X, Moulton HM, Seow Y, Yin H. Fructose Promotes Uptake and Activity of Oligonucleotides With Different Chemistries in a Context-dependent Manner in mdx Mice. Mol Ther Nucleic Acids. 2016 Jun 28;5(6):e329. doi: 10.1038/mtna.2016.46. 
 35.  Takeda K., Sriram S., Derryn Chan XH, Ong WK, Yeo CR, Tan B, Lee SA, Kong KV, Hoon S, Jiang h, Yuen JJ, Perumal J, Agrawal M, Vaz C, So J, Shabbir A, Blaner WS, Olivo M, Han W, Tanavde V, Toh SA, Sugii S. Retinoic acid mediates visceral-specific adipogenic defects of human adipose-derived stem cells. Diabetes (2016) Mar. DOI: 10.2337/db15-1315
 34.  Aw S.S., Tang M. XM, Teo Y. N., Cohen, S. M. A Conformation-induced fluorescence method for microRNA detection.  Nucl. Acids Res. (2016) Mar. DOI: 10.1093/nar/gkw108
 33.  Lee WL, Guo WM, Ho VH, Saha A. Chong HC, Tan NS, Tan EY, Loo SC. Delivery of doxorubicin and paclitaxel from double-layered microparticles: The effects of layer thickness and dual-drug vs. single-drug loading. Acta Biomater. (2015) Sep. DOI:10.1016/j.actbio.2015.08.051.
 32.  Nordin JZ, Lee Y, Vader P, Mäger I, Johansson HJ, Heusermann W, Wiklander OP, Hällbrink M, Seow Y, Bultema JJ, Gilthorpe J, Davies T, Fairchild PJ, Gabrielsson S, Meisner-Kober NC, Lehtiö J, Smith CI, Wood MJ, Andaloussi SE. Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine. (2015) May;11(4):879-83.
 31.  Wiklander OP, Nordin JZ, O'Loughlin A, Gustafsson Y, Corso G, Mäger I, Vader P, Lee Y, Sork H, Seow Y, Heldring N, Alvarez-Erviti L, Smith CE, Le Blanc K, Macchiarini P, Jungebluth P, Wood MJ, Andaloussi SE. Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting. J. Extracell Vesicles. (2015) Apr 20;4:26316.
 30.  Kock K. H., Kong K. W., Hoon S., Seow Y. Functional VEGFA Knockdown with Artificial 3'-tailed mirtrons defined by 5' splice site and branch point. Nucleic Acids. Res. (2015) 
 29.

 Teo Y., Hoon S., Guerette P. A., Wei W., Ghadban A., Hao C., Miserez A., Waite J. H. Infiltration of Chitin by Protein Coacervates Defines The Squid Beak Mechanical Gradient. Nat. Chem. Biol. (2015) doi: 10.1038/nchembio.1833

 28. 

 Teo, K.K.A., Tsuneyoshi, N., Hoon, S., Tan, E.-K., Stanton, L.W., Wright, C.V., and Dunn, N.R. PDX1 Binds and Represses Hepatic Genes to Ensure Robust Pancreatic Commitment in Differentiating Human Embryonic Stem CellsStem Cell Reports 4, 1-13. (2015)

 27.

S. K. Yen, D. P. Varma, W. M. Guo, V. H. B. Ho, V. Vijayaragavan, P. Padmanabhan , K. K. Bhakoo, S. T. Selvan, Synthesis of Small Sized, Porous and Low-toxic Magnetite Nanoparticles by Thin POSS Silica Coating. Chemistry-A European Journal (2015)

 26.

Guo WM, Kong KW, Brown CJ, Quah ST, Yeo HL, Hoon S, Seow Y.Identification and Characterization of an eIF4e DNA Aptamer That Inhibits Proliferation With High Throughput Sequencing. Mol Ther Nucleic Acids. (2014) doi: 10.1038/mtna.2014.70.

 25.

 Lee, Z. Y., Raghavan, S. S., Ghadessy, F. J. & Teo, Y. N. Rapid and sensitive detection of acrylic acid using a novel fluorescence assay. RSC Advances (2014). doi:10.1039/C4RA12495B.

 24.

Lee, W. L., Guo, W. M., Ho, V. H. B., Saha, A., Chong, H. C., Tan, N. S., Widjaja, E., Tan, E. Y. and Loo, S. C. J. (2014), Inhibition of 3-D Tumor Spheroids by Timed-Released Hydrophilic and Hydrophobic Drugs from Multilayered Polymeric Microparticles. Small 2014, 10: 3986–3996.

 23. Dawei Ding, Paul A. Guerette , Shawn Hoon , Kiat Whye Kong , Tobias Cornvik , Martina Nilsson , Akshita Kumar , Julien Lescar , and Ali Miserez., Biomimetic Production of Silk-Like Recombinant Squid Sucker Ring Teeth Proteins.  Biomacromolecules, Article ASAP

doi10.1021/bm500670r.  July 28, 2014.

22. 

Paul A. Guerette, Shawn Hoon, Dawei Ding, Shahrouz Amini, Admir Masic, Vydianathan Ravi, Byrappa Venkatesh, James C. Weaver, and Ali Miserez., Nanoconfined β-Sheets Mechanically Reinforce the Supra-Biomolecular Network of Robust Squid Sucker Ring Teeth. ACS Nano Article ASAP June 09,(2014) .

21.

Guo, W. M., Loh, X. J., Tan, E. Y., Loo, S. C. J., & Ho, V. H. (2014). Development of a magnetic 3D spheroid platform with potential application for high-throughput drug screening. Molecular pharmaceutics, 11 (7), 2182-2189

20.

Walter L. Goh , Min Yen Lee , Thomas L. Joseph , Soo Tng Quah , Christopher J. Brown , Chandra Verma , Sydney Brenner , Farid John Ghadessy , and Yin Nah Teo.,“Molecular rotors as conditionally fluorescent labels for rapid detection of biomolecular interactions.” Journal of the American Chemical Society .

19.

Guerette, P. A., Z Tay, G., Hoon, S., Loke, J. J., Hermawan, A. F., Schmitt, C. N. Z., Harrington, M. J., Masic, A., Karunaratne, A., Gupta, H. S., Tan, K. S., Schwaighofer, A., Nowak, C., and Miserez, A.,“Integrative and comparative analysis of coiled-coil based marine snail egg cases – a model for biomimetic elastomers.” Biomaterials Science (2014).

18.

Venkatesh B, Lee A, Vydianathan R, Lian M, Maurya A, Swann J, Ohta Y, Flajnik M, Sutoh Y, Kasahara M, Hoon SGangu V, Roy S, Irimia M, Korzh V, Kondrychyn I, Tay B, Tohari, S, Lim, Z, Kong KWHo SF, Lorente-Galdos B, Quilez J, Marques-Bonet T, Raney B, Ingham P, Tay A, Hillier L, Minx P, Boehm T, Wilson R, Brenner S, Warren W, “ “Elephant shark genome provides unique insights into gnathostome evolution”. Nature 505, 174–179 (2014).

17.

Lovci MT, Ghanem D, Marr H, Arnold J, Gee S, Parra M, Liang TY, Stark TJ, Gehman LT, Hoon S, Massirer KB, Pratt GA, Black DL, Gray JW, Conboy JG, Yeo GWRbfox proteins regulate alternative mRNA splicing through evolutionarily conserved RNA bridges”.Nature Structural & Molecular Biology 

16.

Guerette P.A., Hoon SSeow Y, Raida M, Masic A, Wong F.THo V H.BKong K W, Demirel M.C, Pena-Francesch A, Amini S,Tay G.Z, Ding D & Ali Miserez. ““Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science”“. Nature Biotechnology 31, 908–915 .

15.

Yen S K, Janczewski D, Lakshmi J L, Dolmanan S B, Tripathy S, Ho V H.B., Vijayaragavan V, Hariharan A, Padmanabhan P, Bhakoo K, Sudhaharan T, Ahmed S, Zhang Y, Tamil Selvan S. “Design and Synthesis of Polymer Functionalized NIR Fluorescent Dyes–Magnetic Nanoparticles for Bioimaging”. ACS Nano. (2013);7(8), 6796–6805.

14.

Ho V H.B.Guo W. M., Huang C. L., Ho S. F., Chan S. Y., Tan E. Y., Ng K. W. & Loo J S.C. “Manipulating magnetic 3D spheroids in hanging drops for applications in tissue engineering and drug screening”. Advanced Healthcare Materials. (2013);2: 1430–1434.

13.

Seow Y., Sibley CR. & Wood MJ. “Artificial mirtron-mediated gene knockdown: functional DMPK silencing in mammalian cells”. RNA. (2012) Jul;18(7):1328-37. Epub 2012 May 30. PubMed PMID: 22647847; PubMed Central PMCID: PMC3383964.

12.

Sibley CR., Seow Y., Curtis H., Weinberg MS. & Wood MJ. “Silencing of Parkinson’s disease-associated genes with artificial mirtron mimics of miR-1224″. Nucleic Acids Res. 

11.

El-Andaloussi S., Lee Y., Lakhal-Littleton S., Li J., Seow Y., Gardiner C., Alvarez-Erviti L., Sargent IL. & Wood MJ. “Exosome-mediated delivery of siRNA in vitro and in vivo”. Nat Protoc. (2012) Nov 15;7(12):2112-26. Epub 2012 Nov 15. PubMed PMID: 23154783.

10.

Lagier-Tourenne C., Polymenidou M., Hutt KR., Vu AQ., Baughn M., Huelga SC., Clutario KM., Ling SC., Liang TY., Mazur C., Wancewicz E., Kim AS., Watt A., Freier S., Hicks GG., Donohue JP., Shiue L., Bennett CF., Ravits J., Cleveland DW. & Yeo GW. “Divergent roles of ALS-linked proteins FUS/TLS and TDP-43 intersect in processing long pre-mRNAs”. Nat Neurosci.

9.

Song L., Ho V H.B., Chen C., Yang Z., Liu D., Chen R. and Zhou D.“Efficient, pH-Triggered Drug Delivery Using a pH-Responsive DNA-Conjugated Gold Nanoparticle”. Advanced Healthcare Materials.(2012) Highlighted on the back cover of Advanced Healthcare Materials (Feb 2013).

8.

Wilbert M., Huelga S., Kapeli K., Stark T., Liang T., Chen S., Yan B., Nathanson J., Hutt K., Lovci M., Kazan H., Vu A., Massirer K., Morris Q., Hoon S. & Yeo G“LIN28 Binds Messenger RNAs at GGAGA Motifs and Regulates Splicing Factor Abundance”. Mol. Cell (2012).

7.

Wong K., Chan JM., Kamm RD. & Tien J. “Microfluidic Models of Vascular Functions”. Annual Review Biomedical Engineering(2012). 14:205–30.

6.

 Huelga S., Vu A., Arnold J., Liang T., Liu P., Yan B., Donohue J., Shiue L., Hoon S., Brenner S., Area M. & Yeo GY“Integrative Genome-wide Analysis Reveals Cooperative Regulation of Alternative Splicing by hnRNP proteins”. Cell Reports.

5.

Hoon S., Zhou B., Janda KD., Brenner S.& Scolnick J“Aptamer selection by high-throughput sequencing andinformatic analysis”. Biotechniques;51(6):413-614.

4.

Lissina E., Young B., Urbanus M.L., Guan X.L., Lowenson J., Hoon S., Baryshnikova A., Riezman I., Michaut M., Riezman H., Cowen L.E., Wenk M.R., Clarke S.G., Giaever G. & Nislow C. “A Systems Biology Approach Reveals the Role of a Novel Methyltransferase in Response to Chemical Stress and Lipid Homeostasis.”PLoS Genetics (2011) 7(10).

3.

 Stark T.J., Arnold J.D., Spector D.H. & Yeo G.W., “High-resolution profiling and analysis of viral and host small RNAs during human cytomegalovirus infection”.Journal of Virology (2011).

2.

Sibley C.R., Seow Y., Saayman S., Dijkstra K.K., El Andaloussi S., Weinberg M.S. & Wood M.J., “The biogenesis and characterization of mammalian microRNAs of mirtron origin”.Nucleic Acids research (2011).

1. Pethe K., Sequeira P.C., Agarwalla S., Rhee K., Kuhen K., Phong W.Y., Patel V., Beer D., Walker J.R., Duraiswamy J., Jiricek J., Keller T.H., Chatterjee A., Tan M.P., Ujjini M., Rao S.P., Camacho L., Bifani P., Mak P.A., Ma I., Barnes S.W., Chen Z., Plouffe D., Thayalan P., Ng S.H., Au M., Lee B.H., Tan B.H., Ravindran S., Nanjundappa M., Lin X., Goh A., Lakshminarayana S.B., Shoen C., Cynamon M., Kreiswirth B., Dartois V., Peters E.C., Glynne R., Brenner, S. & Dick T., “A chemical genetic screen in Mycobacterium tuberculosis identifies carbon-source-dependent growth inhibitors devoid of in vivo efficacy”.Nature communications, vol. 1, pp. 57 (2010).