RESEARCH
Transcription and translation are the major steps in controlling gene expression. However, RNA expression abundance only has around 40% explanatory power of protein abundance, suggesting post-transcription regulation such as RNA structure-based regulation.
To date, great interest is growing in the relationship between gene function and RNA structure. For example, RNA structure has been linked to neurological disease. However, unlike DNA and protein structure, RNA is inherently flexible and hence more difficult to study due to technique limitations. One of the bottlenecks of RNA structure study is that it requires millions of cells as starting materials, causing the lagging behind of RNA structure studies. To overcome this problem, we recently developed a single-cell RNA structure sequencing approach to obtain both RNA secondary structure and RNA expression information simultaneously, at single-cell level.
We are very interested in studying the roles of RNA structure in cell developmental processes and diseases using our technologies. Our final goal is to identify RNA structure-based biomarkers in different cell types/stages or novel drug targets in diseases.
Selected Publications
- Jiaxu Wang; Tong Zhang; Yu Zhang; Wen Ting Tan; Ming Wen; Yang Shen; Finnlay R.P. Lambert; Roland G. Huber; Yue Wan ; Genome-wide RNA structure changes during human neurogenesis modulate gene regulatory networks, Molecular Cell, 2021, 81(23): 4942-4953
- Yue, Zhao; Jiaxu, Wang (co-first Author); Wai Nam, Liu; Shin Yie, Fong; Timothy Wai Ho, Shuen; Min, Liu; Sarah, Harden; Sue Yee, Tan; Jia Ying, Cheng; Wilson Wei Sheng, Tan; Jerry, Kok Yen Chan; Cheng Ean, Chee; Guan Huei, Lee; Han Chong, Toh; Seng Gee, Lim; Yue, Wan; Qingfeng, Chen; Analysis and validation of human targets and treatments using a hepatocellular carcinoma - immune humanized mouse model, Hepatology, 2021, 74(3): 1395-1410
- Aw, Jong Ghut Ashley; Lim, Shaun W.; Wang, Jiaxu (co-first Author); Lambert, Finnlay R.P.; Tan, Wen Ting; Shen, Yang; Zhang, Yu; Kaewsapsak, Pornchai; Li, Chenhao; Ng, Sarah B.; Vardy,Leah A.; Tan, Meng How; Nagarajan, Niranjan; Wan, Yue ; Determination of isoform-specific RNA structure with nanopore long reads, Nature Biotechnology, 2021, 39(3): 336-346 (Co-First Author)
- Wang Jiaxu; Jenjaroenpun Piroon; Bhinge Akshay; Angarica Vladimir Espinosa; Del Sol Antonio; Nookaew Intawat; Kuznetsov Vladimir A; Stanton Lawrence W ; Single-cell gene expression analysis reveals regulators of distinct cell subpopulations among developing human neurons, Genome Research, 2017, 27(11): 1783-1794
- Wang Jiaxu; Qiao Mengran; He Qianqian; Shi Ronghua; Loh Sharon Jia Hui; Stanton Lawrence W; Wu Mian ; Pluripotency Activity of Nanog Requires Biochemical Stabilization by Variant Histone Protein H2A.Z, Stem Cells, 2015, 33(7): 2126-2134
- Wang Jiaxu; He Qianqian; Han Chuanchun; Gu Hao; Jin Lei; Li Qun; Mei Yide; Wu Mian ; p53-Facilitated miR-199a-3p Regulates Somatic Cell Reprogramming, Stem Cells, 2012, 30(7): 1405-1413