Protein Analytics

The Protein Analytics laboratory hosts a repertoire of instrumentation for protein identification, quantitative protein expression profiling and mapping of post translational modifications. 2D Gel electrophoresis is used to separate protein mixtures. Replicate gel maps are compared and differentiating proteins are isolated, digested and identified by mass spectrometry (MS). Metabolic labelling of proteins with stable isotopes and peptide isobaric tagging is used to quantitatively compare the expression levels of proteins by MS. Tandem MS is performed to obtain peptide sequence information to identify proteins and to quantify labelled peptides.

proteomics pic

PEOPLE

Bi Xuezhi

Dr. Bi Xuezhi

bi_xuezhi@bti.a-star.edu.sg
Principal Scientist

PhD in Genetics (2000), Wuhan University, China

Research Focus/Interests

  • Mass spectrometry based quantitative and qualitative proteomics
  • Drug target identification and protein post translational modification (PTMs) as well as protein-protein interacting analysis
  • Proteomic profiling of upstream and downstream processes of recombinant protein production in mammalian cells
  • Biopharmaceutical multiple critical quality attributes (CQAs) analysis by mass spectrometry
kok yee jiun

Dr. Kok Yee Jiun

kok_yee_jiun@bti.a-star.edu.sg
Staff Scientist

PhD in Biochemistry (2016), National University of Singapore, Singapore

Research Focus/Interests

  • Qualitative and quantitative proteomics profiling of mammalian production cell lines
  • Mass spectrometry-based characterization of recombinant therapeutic proteins
  • Identification and characterization of disease-associated biomarkers
Alok Patra

Dr. Alok Tanala Patra

tanala_patra_alok@bti.a-star.edu.sg
Research Scientist

PhD in Chemical Biology and Drug Discovery (2021), Singapore University of Technology and Design, Singapore

Research Focus/Interests

  • MS-based Quantitative Proteomics profiling for mammalian cell lines and protein quality attributes characterization
  • Protein biomarker discovery and characterization using modern separations coupled with advanced mass spectrometry (MS)
  • Process analytical technology (PAT) in biotherapeutics and alternative proteins in the biomanufacturing process
Noorhamizah Suhaimi

Dr. Noorhamizah Suhaimi

noorhamizah_suhaimi@bti.a-star.edu.sg
Research Scientist

PhD in Bioscience (2020), Shizuoka University, Shizuoka Prefecture, Japan

Research Focus/Interests

  • Characterization of antigen protein display on envelope and non-envelope protein platforms for nanoparticle vaccines
  • One-step affinity chromatography binding antigen protein optimization
Tan Chee Fan

Dr. Tan Chee Fan

tan_chee_fan@bti.a-star.edu.sg
Research Scientist

PhD in Systems Biology and Medicine (2020), Nanyang Technological University, Singapore

Research Focus/Interests

  • Characterization of dietary protein content using mass spectrometry based techniques
  • Optimization of LC-MS/MS-based quantitative proteomics analysis for biologics development
Kwan Soon Hong

Dr. Sebastian Kwan

sebastian_kwan@bti.a-star.edu.sg
Project Scientist

PhD in Drug Discovery and Proteomics (2018), Universiti Sains Malaysia, Malaysia

Research Focus/Interests

  • Identification and characterisation of novel proteins in natural products
  • Elucidation of bioactive proteins using advanced LC/MS-MS mass spectrometry
  • Effective isolation and separation of proteins through innovative electrophoresis