Protein Analytics

The Protein Analytics laboratory hosts a repertoire of instrumentation for protein identification, quantitative protein expression profiling and mapping of post translational modifications. 2D Gel electrophoresis is used to separate protein mixtures. Replicate gel maps are compared and differentiating proteins are isolated, digested and identified by mass spectrometry (MS). Metabolic labelling of proteins with stable isotopes and peptide isobaric tagging is used to quantitatively compare the expression levels of proteins by MS. Tandem MS is performed to obtain peptide sequence information to identify proteins and to quantify labelled peptides.

proteomics pic


Bi Xuezhi

Dr. Bi Xuezhi
Principal Scientist

PhD in Genetics (2000), Wuhan University, China

Research Focus/Interests

  • Mass spectrometry based quantitative and qualitative proteomics
  • Drug target identification and protein post translational modification (PTMs) as well as protein-protein interacting analysis
  • Proteomic profiling of upstream and downstream processes of recombinant protein production in mammalian cells
  • Biopharmaceutical multiple critical quality attributes (CQAs) analysis by mass spectrometry
kok yee jiun

Dr. Kok Yee Jiun
Senior Staff Scientist

PhD in Biochemistry (2016), National University of Singapore, Singapore

Research Focus/Interests

  • Qualitative and quantitative proteomics profiling of mammalian production cell lines
  • Mass spectrometry-based characterization of recombinant therapeutic proteins
  • Identification and characterization of disease-associated biomarkers
Tan Chee Fan

Dr. Tan Chee Fan
Associate Staff Scientist

PhD in Systems Biology and Medicine (2020), Nanyang Technological University, Singapore

Research Focus/Interests

  • Characterization of dietary protein content using mass spectrometry based techniques
  • Optimization of LC-MS/MS-based quantitative proteomics analysis for biologics development
Alok Patra

Dr. Alok Tanala Patra
Research Scientist

PhD in Chemical Biology and Drug Discovery (2021), Singapore University of Technology and Design, Singapore

Research Focus/Interests

  • MS-based Quantitative Proteomics profiling for mammalian cell lines and protein quality attributes characterization
  • Protein biomarker discovery and characterization using modern separations coupled with advanced mass spectrometry (MS)
  • Process analytical technology (PAT) in biotherapeutics and alternative proteins in the biomanufacturing process
Mauro Galli

Dr. Mauro Galli
Research Scientist

PhD in Medical and Health Sciences (2022), Medical University of Bialystok, Poland

Research Focus/Interests

  • Untargeted proteomics and multi-omics in biologics and bioprocessing field
  • Innovation and development of data-independent acquisition (DIA) proteomics
Noorhamizah Suhaimi

Dr. Noorhamizah Suhaimi
Research Scientist

PhD in Bioscience (2020), Shizuoka University, Shizuoka Prefecture, Japan

Research Focus/Interests

  • Characterization of antigen protein display on envelope and non-envelope protein platforms for nanoparticle vaccines
  • One-step affinity chromatography binding antigen protein optimization