Cellular Immunotherapies
![DEVELOPMENT OF PRE-GMP PROCESSES DEVELOPMENT OF PRE-GMP PROCESSES](/images/librariesprovider7/default-album/development-of-pre-gmp-processes7e13f106913d45e390c41c282ffcce90.png?sfvrsn=f32eb475_0)
DEVELOPMENT OF PRE-GMP PROCESSES
![CAR Engineering CAR Engineering](/images/librariesprovider7/default-album/car-engineering9e30563a908047389cd1dbacb2e3e848.png?sfvrsn=d5b0114a_0)
Car Engineering
![FEEDER CELL LINE ENGINEERING FEEDER CELL LINE ENGINEERING](/images/librariesprovider7/default-album/feeder-cell-line-engineering543ed85d71594218959b21d961c28d60.png?sfvrsn=4679a25_0)
FEEDER CELL LINE ENGINEERING
![ALLOGENEIC CELLULAR IMMUNOTHERAPIES ALLOGENEIC CELLULAR IMMUNOTHERAPIES](/images/librariesprovider7/default-album/allogeneic-cellular-immunotherapies6442b0a1092b41bea384bd9746843584.png?sfvrsn=d069f19b_0)
ALLOGENEIC CELLULAR IMMUNOTHERAPIES
![VIRAL VECTOR PRODUCTION VIRAL VECTOR PRODUCTION](/images/librariesprovider7/default-album/viral-vector-production6863ead4b2b64852a36a8dc520adb5cc.png?sfvrsn=57d5a24a_0)
VIRAL VECTOR PRODUCTION
![COMPREHENSIVE INSIGHTS NK T CELL PRODUCTION COMPREHENSIVE INSIGHTS NK T CELL PRODUCTION](/images/librariesprovider7/default-album/comprehensive-insights-nk-t-cell-productioncd2e6db6ad53417195bf596d500e60c7.png?sfvrsn=397d2c00_0)
COMPREHENSIVE INSIGHTS: NK/T CELL PRODUCTION
Manufacturing of Cellular Immunotherapies
![PD of Manufactg of Autologous CAR-T Immunotherapies PD of Manufactg of Autologous CAR-T Immunotherapies](/images/librariesprovider7/default-album/pd-of-manufactg-of-autologous-car-t-immunotherapies.jpg?sfvrsn=545ac66e_0)
Autologous alpha beta T cells
- Platform for CAR engineering and expansion: CAR engineering of ab T cells and their expansion utilising suitable bioreactors to generate pre-GMP bank from different donors.
- Immunophenotypic and functional characterisation: To investigate anti-tumour function and functional persistence or exhaustion of cells via flow cytometric and bioluminescence methods.
![Allogeneic Allogeneic](/images/librariesprovider7/default-album/allogeneic-v2.jpg?sfvrsn=f9dfd872_0)
Allogeneic NK or gamma delta T cells
- Allogeneic Production: HLA-Unrestricted: NK / gd T cells derived from cord / peripheral blood, modified or not with CAR and expanded
- Enhanced expansion and anti-tumour potency: Engineering of feeder cells to provide specific signals that stimulate expansion of NK / gd T cells co-cultured with irradiated feeder cells
- Tumour-targeting efficiency: Transduction of NK / gd T cells with rationally designed CARs
- Clinically relevant numbers: GMP-compliant processes used to generate allogeneic cell products
- Efficient product assessment: Collaboration with other teams to establish CQAs of final product that positively correlate with its functional efficacy
- Clinical translation: Pre-GMP banking of CAR VPCs / RVVs, engineered feeder cells, CAR-NK / T cells
Cultivated Meat
![Isolation and in vitro culture of stem cells for cultivated seafood Isolation and in vitro culture of stem cells for cultivated seafood](/images/librariesprovider7/default-album/isolation-and-in-vitro-culture-of-stem-cells-for-cultivated-seafood.png?sfvrsn=4e3f2f12_0)
Our work is part of CRISP Meats, an ongoing multi-institutional programme funded by the Singapore Food Story R&D Programme to develop novel technologies for the production of cultivated meat through public-private partnerships.
Our work includes:
- Isolation of stem cells from various animal species
- Optimisation of culture conditions
- Immortalisation of cells
- Characterisation of cell lines
Messenger RNA
![Analytical testing of mRNA-based therapeutics and prophylactics Analytical testing of mRNA-based therapeutics and prophylactics](/images/librariesprovider7/default-album/analytical-testing-of-mrna-based-therapeutics-and-prophylactics.png?sfvrsn=31543b1c_0)
- Develop and validate analytical methods: For characterising the identity, purity and potency of mRNA-based therapeutics which complement capabilities in mRNA manufacturing
Our Track Record
Featured publications:
- Chelsia Qiuxia Wang, Fong Chan Choy, Arleen Sanny, Takashi Murakami, Andy Hee-Meng Tan & Kong-Peng Lam (2023) An Inhibitory Role for Human CD96 Endodomain in T Cell Anti-Tumor Responses. Cells 12: 309
- Zhenzhong Chen*, Seokgyu Han*, Arleen Sanny, Dorothy Leung-Kwan Chan, Danny van Noort, Wanyoung Lim, Andy Hee-Meng Tan & Sungsu Park (2022) 3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay. Journal of Nanobiotechnology 20: 1–14
- Andy Hee-Meng Tan, Alice Man Sze Cheung & Dieter Kabelitz (2022) Editorial: Next generation gd T cell-based tumor immunotherapy. Frontiers in Immunology 13: 1041362
- Chelsia Qiuxia Wang & Andy Hee-Meng Tan (2021) Driving CARs on the biomanufacturing road to clinical success. Cell and Gene Therapy Insights 7: 1613–1621
- Alison P Lee*, Yee Jiun Kok*, Meiyappan Lakshmanan*, Dawn Leong, Lu Zheng, Hsueh Lee Lim, Shuwen Chen, Shi Ya Mak, Kok Siong Ang, Neil Templeton, Taha Salim, Xiaona Wei, Eric Gifford, Andy Hee-Meng Tan, Xuezhi Bi, Say Kong Ng, Dong-Yup Lee, Wai Lam W Ling & Ying Swan Ho (2021) Multi-omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer, and product quality. Biotechnology and Bioengineering 118(11): 4305–4316
The Team
![Andy Tan Andy Tan](/images/librariesprovider7/default-album/dr-andy-tan.jpg?sfvrsn=89bfabb2_0)
Dr Andy Tan
andy_tan@bti.a-star.edu.sg
Principal Scientist II
PhD in Immunology (2008), National University of Singapore Graduate School for Integrative Sciences and Engineering, Singapore
Research Focus / Interest
- Process development for manufacturing of autologous and allogeneic cellular immunotherapies
- Development of cell lines from food animal species for cultivated meat applications
- Nucleic Acid- and Immuno-Analytics for characterisation of RNA prophylactics and therapeutics
![alison lee alison lee](/images/librariesprovider7/research/tra-scriptomics/alison-lee.jpg?sfvrsn=69e57d0c_2)
Dr Alison Lee
alison_lee@bti.a-star.edu.sg
Principal Scientist I
PhD in Bioinformatics (2009), NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore
Research Focus / Interest
- Transcriptomics and epigenomics of cell factories of biotechnological interest such as Chinese Hamster Ovary (CHO) cell lines to identify crucial regulatory networks influencing product quality and yield
- Cultivated meat production and safety assessment
- mRNA characterisation and analysis
![Chelsia Wang Chelsia Wang](/images/librariesprovider7/default-album/chelsia-wang.jpg?sfvrsn=836f87f6_0)
Dr Chelsia Wang
chelsia_wang@bti.a-star.edu.sg
Senior Scientist II
PhD in Biological Sciences (2012), National University of Singapore, Singapore
Research Focus / Interest
- Process development for manufacturing of autologous and allogeneic cellular immunotherapies
- Therapies targeting solid tumours and leukemias
![Yang Lei Yang Lei](/images/librariesprovider7/research/immune-engineering/yang-lei.jpg?sfvrsn=aebff710_2)
Dr Yang Lei
yang_lei@bti.a-star.edu.sg
Senior Scientist I
PhD in Immunology (2019), National University of Singapore, Singapore
Research Focus / Interest
- Cancer immunotherapy
- Translational research
![Yu Zhao Yu Zhao](/images/librariesprovider7/default-album/yu-zhao.jpg?sfvrsn=5975057f_0)
Dr Zhao Yu
yu_zhao@bti.a-star.edu.sg
Senior Scientist I
PhD in Pharmacology (2010), Academy of Military Medical Science, China
Research Focus / Interest
- Investigation and Development cellular/genetic therapeutic products for anti-tumor therapy
- The interaction and regulatory network between tumour microenvironment and immune cells in the solid tumours
- Process development and optimization of cellular/genetic therapeutic products manufacturing
![Danai Georgiadou2 Danai Georgiadou2](/images/librariesprovider7/default-album/danai-georgiadou2.jpg?sfvrsn=646a6717_0)
Dr Danai Georgiadou
danai_georgiadou@bti.a-star.edu.sg
Scientist
PhD in Human Development and Embryology (2022), University of Amsterdam, The Netherlands
Research Focus / Interest
- Investigating the influence of diverse culture conditions and growth factors on the development of high-quality cultivated meat cell lines
- Understanding the interactions between different cell types in tissues from various species for improved functionality and growth
- Exploring the viability and scalability of alternative cell sources for cultivated meat production
- Employing stem cell-based methodologies to augment the efficiency and productivity of primary cell isolation processes
![Samantha Nguee Samantha Nguee](/images/librariesprovider7/default-album/samantha-nguee.jpg?sfvrsn=835e1c8d_0)
Dr Samantha Nguee
samantha_nguee@bti.a-star.edu.sg
Scientist
PhD in Microbiology and Immunology (2021), Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Research Focus / Interest
- Process development for manufacturing of cellular immunotherapies
- Optimization and validation of cellular immunotherapies
![Toh Jia Ying Toh Jia Ying](/images/librariesprovider7/default-album/toh-jia-ying.jpg?sfvrsn=4a2ff12a_0)
Dr Toh Jia Ying
toh_jia_ying@bti.a-star.edu.sg
Scientist
PhD in Immunology (2023), Stanford University, United States of America
Research Focus / Interest
- Development and optimisation of manufacturing processes for allogeneic cellular immunotherapies against cancer
- Immunophenotyping and characterisation of cellular immunotherapy products