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Our Capabilities
CULTURE MEDIA DEVELOPMENT
In-house serum free basal and feed media formulations
BATCH, FED-BATCH, CONTINUOUS AND PERFUSION BIOREACTOR PROCESS DESIGN
Efficient, high-titer and scalable animal cell cultivation processes from 10 mL to 50 L
MEDIA AND PROCESS OPTIMISATION USING MULTI-OMICS ANALYSIS
In partnership with BTI’s Analytical Science & Technology (AS&T) research groups
Control & Feed Strategies
Optimized process parameter and feeding strategies to improve product yield and quality
Sampling & Monitoring
Automated/Manual sampling of bioreactor cultures for monitoring of critical process parameters
Aseptic Handling & Bioreactor Operation
Aseptic manipulation throughout seed-train and bioreactor process
These capabilities enable research translation by facilitating scalable manufacturing and commercialization of products made using animal cell culture, such as recombinant proteins, viral vectors, vaccines and cultivated meats.
Our Technologies
Our team develops technologies to enable the use of animal cells for different applications. Our in-house media component screening platform has enabled us to develop several serum-free cell culture media formulations for various cultivation modes.
Examples of our Technologies
Some technologies we have developed are listed below:
- Suspension bioreactor process for adeno-associated viral vector production
- GLUL-KO HEK293 bioproduction system
- Proprietary serum-free media formulations for CHO, HEK, NS0, chicken and fish cell lines
- Media component screening and optimization platform and know-hows
- Optimised transient gene expression processes
- Animal virus propagation processes
- Bioreactor set-up and process control strategies for fed-batch and perfusion cultures
- Method of increasing protein yield of a serum free mammalian cell culture using maltose as a carbohydrate supplement
- Production of Alpha-1-antitrypsin, Alkaline phosphatase and Erythropoietin
GLUL-KO HEK293 bioproduction system that enables the production of recombinant proteins with decent 1 g/L titer in a human cell line.
Optimised process for rAAV production using Triple plasmid transfection with PEI, that does not require media replacement after transfection, and is validated in 2L stirred tank bioreactors.
OUR TRACK RECORD
Featured Publications
- Evan Tan, Cara Sze Hui Chin, Zhi Feng Sherman Lim, Say Kong Ng (2021) HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Frontiers in Bioengineering and Biotechnology 9: 796991
- Yin Ying Ho, Hao Kim Lu, Zhi Feng Sherman Lim, Hao Wei Lim, Ying Swan Ho, Say Kong Ng (2021) Applications and Analysis of Hydrolysates in Animal Cell Culture. Bioresources and Bioprocessing 8(1): 93
- Alison P. Lee, Yee Jiun Kok, Meiyappan Lakshmanan, Dawn Leong, Lu Zheng, Hsueh Lee Lim, Shuwen Chen, Shi Ya Mak, Kok Siong Ang, Neil Templeton, Taha Salim, Xiaona Wei, Eric Gifford, Andy Hee‐Meng Tan, Xuezhi Bi, Say Kong Ng, Dong‐Yup Lee, Wai Lam W. Ling and Ying Swan Ho (2021) Multi‐omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer, and product quality. Biotechnology and Bioengineering 118(11): 4305-4316
- Christine Lin Chin, Justin Bryan Goh, Harini Srinivasan, Kaiwen Ivy Liu, Ali Gowher, Raghuvaran Shanmugam, Hsueh Lee Lim, Matthew Choo, Wen Qin Tang, Andy Hee-Meng Tan, Terry Nguyen-Khuong, Meng How Tan and Say Kong Ng (2019) A human expression system based on HEK293 for the stable production of recombinant erythropoietin. Scientific Reports 9: 16768
- Dawn Sow Zong Leong, Brian Kah Hui Teo, Janice Gek Ling Tan, Hayati Kamari, Yuan Sheng Yang, Peiqing Zhang and Say Kong Ng (2018) Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody. Scientific Reports 8: 4037
The Team
Dr Ng Say Kong
ng_say_kong@bti.a-star.edu.sg
Senior Principal Scientist I
PhD in Molecular Engineering of Biological and Chemical Systems (2006) National University of Singapore, Singapore
Research Focus / Interest
- Culture medium and bioreactor process developments to improve the bioproduction using animal cells
- Development of perfusion culture processes
- Recombinant protein expression in mammalian cells
- Production of viral vaccines and viral vectors
- Reducing upstream production costs for cultivated meats
Dr Evan Tan
evan_tan@bti.a-star.edu.sg
Senior Scientist II
PhD in Molecular and Cell Biology (2017), National University of Singapore, Singapore
Research Focus / Interest
- Recombinant adeno-associated virus (rAAV) production
- Engineering of mammalian cells for rAAV production
Dr Eunice Jiayu Leong
eunice_leong@bti.a-star.edu.sg
Senior Scientist I
PhD in Chemical Engineering (2018), University of Illinois at Urbana-Champaign, USA
Research Focus / Interest
- Bioreactor process development for producing biologics from animal cell cultures
- Recombinant protein expression in mammalian cells
Dr Quek Jun Ping
quek_jun_ping@bti.a-star.edu.sg
Senior Scientist I
PhD in Molecular and Structural Biology (2022), Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
Research Focus / Interest
- Culture medium and bioreactor process developments for cultivated meats
- Production of viral vaccines
Dr Tan Yu Xuan
tan_yu_xuan@bti.a-star.edu.sg
Scientist
PhD in Developmental Neuroscience (2023), Francis Crick Institute, King’s College London, UK
Research Focus / Interest
- Cell-type differentiation and bioreactor process development for cultivated meats
Dr Viknesvaran Selvarajan
viknesvaran_selvarajan@bti.a-star.edu.sg
Scientist
PhD in Cancer Biology (2016), National University of Singapore, Singapore
Research Focus / Interest
- Viral vector production and purification
- Allogeneic NK cell production process development
