Animal Cell Bioprocessing

    LOWERING COST OF GOODS & SCALING-UP FOR BIOMANUFACTURING

    The market demand for animal cell culture remains strong. This is driven by the high dosage requirements of biotherapeutic drugs such as antibodies for immunotherapies and viral vector for gene therapies. The worldwide quest for cruelty-free, low-cost and environmentally friendly cultivated meats recently adds a new perspective to animal cell culture. These applications have created a need for efficient, high-titer processes which dramatically reduce the cost of goods during manufacturing.

    The Animal Cell Bioprocessing Group develops efficient, high-titer and scalable animal cell cultivation processes to enable scale-up manufacturing for these applications to reduce cost of goods. Using our capabilities in upstream animal cell culture processing with bioreactors of different make, we develop scalable batch, fed-batch, continuous and perfusion bioreactor cell culture processes from 10 mL to 50 L. To further improve protein productivity and product yields, chemically defined protein-free media and optimized feeding strategies for recombinant therapeutic protein production using Chinese Hamster Ovary (CHO) and other mammalian cell lines have also been established. Using new technologies such as Metabolomics, intra- and extra-cellular metabolites will be tracked in high density cultures, which can guide media design for improved culture performance. These capabilities enable research translations by facilitating scalable manufacturing and commercialization of products made using animal cell culture, such as recombinant proteins, viral vectors, vaccines and cultivated meats.

    The group is actively researching in continuous bioreactor processes for antibody production, process development for AAV and heparan sulfate production, development of HEK293 bioproduction system, and production of cultivated meats.

Eqpt used by Animal Cell Bioprocessing

People

Ng Say Kong

Dr. Ng Say Kong

ng_say_kong@bti.a-star.edu.sg
Principal Scientist

PhD in Molecular Engineering of Biological and Chemical Systems (2006) National University of Singapore, Singapore

Research Focus/Interests

  • Culture medium and bioreactor process developments to improve the bioproduction using animal cells
  • Development of perfusion culture processes
  • Recombinant protein expression in mammalian cells
  • Production of viral vaccines and viral vectors
  • Reducing upstream production costs for cultivated meats
Eunice Leong

Dr. Eunice Jiayu Leong

eunice_leong@bti.a-star.edu.sg
Associate Staff Scientist

PhD in Chemical Engineering (2018), University of Illinois at Urbana-Champaign, USA

Research Focus/Interests

  • Bioreactor process development for producing biologics from animal cell cultures
  • Recombinant protein expression in mammalian cells
Evan Tan

Dr. Evan Tan

evan_tan@bti.a-star.edu.sg
Associate Staff Scientist

PhD in Molecular and Cell Biology (2017), National University of Singapore, Singapore

Research Focus/Interests

  • Recombinant adeno-associated virus (rAAV) production
  • Engineering of mammalian cells for rAAV production
Quek Jun Ping

Dr. Quek Jun Ping

quek_jun_ping@bti.a-star.edu.sg
Research Scientist

PhD in Molecular and Structural Biology (2022), Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore

Research Focus/Interests

  • Culture medium and bioreactor process developments for cultivated meats
  • Production of viral vaccines
Viknesvaran Selvarajan

Dr. Viknesvaran Selvarajan

viknesvaran_selvarajan@bti.a-star.edu.sg
Research Scientist

PhD in Cancer Biology (2016), National University of Singapore, Singapore

Research Focus/Interests

  • Viral vector production and purification
  • Allogeneic NK cell production process development