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Our Capabilities
Reduced time
Obtaining high producing clones within 9 weeks
High Titre
High titre of ~5g/L (mabs)
Stable Cell Line
Stable production >60 generations
Rapid developability screening
Time and cost savings
Customizing gene expression
Customizable vector for altering gene expression
Tailored production of recombinant proteins
CHO expression system for producing high-value protein-based materials
Performance of a biosimilar–producing CHO cell line generated using our multi-cistronic vector technology
.jpg?sfvrsn=66e341b7_0 "Titre Graph")
.jpg?sfvrsn=22ca523a_0 "High Viable Cell Density")
(Data collected in 14-day bioreactor fed-batch cultures)
Our Technologies
“Swap and switch” targeted integration technology
- Site-directed: Gene of interest inserted into active recombination sites in genome
- Precision: Control expression level of multiple genes
- Simplicity: Reduce complicated process of screening many clones
Multicistronic vector for biologics expression
- Accuracy: Minimise non-expressing clones with tight selection strategy
- Enhancement: Enhanced and optimised gene expression
.jpg?sfvrsn=de336367_0 "CHO Simultaneous Display and Secretion (CHO SDAP)")
CHO Simultaneous Display and Secretion (CHO SDAP)
- Dual functionality: Simultaneous screening and production in the same host cells
- Big library size: >10M antibodies
Precision metabolic engineering
- Modify antibody function (left): Produce antibodies with specific glycan modifications, which leads to increased ADCC or CDC activity.
- Customized Heparan sulfate (right): Produced a CHO-derived HS that can increase cell proliferation in ex-vivo human mesenchymal stem cell cultures.
Our Track Record
Featured publications:
- Tran Bich Ngan Nguyen, Jianer Lin, Shi Jie Tay, Jessna Yeo, Terry Nguyen-Khuong and Yuansheng Yang (2021) Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans. Scientific Reports 11(1): 12969.
- Jessna Yeo, Mariati and Yuansheng Yang (2018) An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High-Level Monoclonal Antibody Producing CHO DG44 Cell Lines. Methods in Molecular Biology, 1827: 335–349.
- Jessna Yeo, Steven Ho, Mariati, Esther Koh, Shi Jie Tay, Susanto Woen, Peiqing Zhang and Yuansheng Yang (2017) Optimized Selection Marker and CHO Host Cell Combinations for Generating High Monoclonal Antibody Producing Cell Lines. Biotechnology Journal 12(12): 10.1002/biot.201700175.
- Jian'er Lin, Shu Hui Neo, Steven C. L. Ho, Jessna H. M. Yeo, Tianhua Wang, Wei Zhang, Xuezhi Bi, Sheng-Hao Chao and Yuansheng Yang (2017) Impact of Signal Peptides on Furin-2A Mediated Monoclonal Antibody Secretion in CHO Cells. Biotechnology Journal 12(9): 10.1002/biot.201700268
- Steven Ho, Esther Koh, Benjamin Soo, Mariati, Sheng-Hao Chao and Yuansheng Yang (2016) Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells. BMC Biotechnology 16(1): 71
Landmark patent & IP:
- Novel promoters for high level expression (2020)
- Modified human CMV promoters that are resistant to gene silencing (2015)
- IRES mediated multicistronic vectors (2014)
The Team
Dr Yang Yuansheng
yang_yuansheng@bti.a-star.edu.sg
Senior Principal Scientist II
PhD in Chemical Engineering (2005) Vanderbilt University, USA
Research Focus / Interest
- Vector design, cell line development, cell engineering, and bioprocess development to enhance production of recombinant protein production in mammalian cells
Dr Loh Han Ping
loh_han_ping@bti.a-star.edu.sg
Senior Scientist I
PhD in Biological Sciences (2019), Nanyang Technological University, Singapore
Research Focus / Interest
- Cell line development for the production of heparan sulphate/ heparin in mammalian cells
Dr Foong Choon Pin
foong_choon_pin@bti.a-star.edu.sg
Scientist
PhD in Microbial Biotechnology (2016), Universiti Sains Malaysia, Malaysia
Research Focus / Interest
- Engineering mammalian cells for the production of recombinant antibodies
Dr Jessica Ng
jessica_ng@bti.a-star.edu.sg
Scientist
PhD in Biochemistry (2024), Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Research Focus / Interest
- TCR-based T-cell engaging bispecific antibodies
- Jurkat cell line engineering
Dr Wong Yu Qian
wong_yu_qian@bti.a-star.edu.sg
Scientist
PhD in Biochemistry (2016), National University of Singapore, Singapore
Research Focus / Interest
- Nanobody discovery, engineering and production
- Bispecific antibody engineering
