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Our Capabilities
Microcarriers
- Accelerated reprogramming
- Robust
- Scalable
- High yield
Stem Cell Media Formulation
- Serum-free
- Xeno-free
Stem Cell Differentiation
- CRISPR activation-mediated
- Microcarrier-based
- High yield
Our Technologies
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All-In-One Platform for Reprogramming on Microcarriers (RepMC)
- Speed: Accelerate reprogramming by 7 days faster than monolayer
- Robust: Ability to expand and efficiently differentiate to the three germ-layers, heart cells and blood cells
- Various Cell Sources: fibroblasts, peripheral blood mononuclear cells, T cells and CD34+ blood stem cells
- Automation Ready: Platform is also amenable to automation for processing more donor samples in a small footprint, allowing robotic processes to replace manual handling
Microcarrier-based Bioreactor System
- Robust: tested and proven in human pluripotent and mesenchymal stem cell expansion
- Scalable: up to 5L while maintaining pluripotency of stem cells
- High yield: 3 to 10-fold higher than conventional 2D cultures
Our Track Record
Featured Publications
- Alan Tin Lun Lam, Valerie Ho, Svetlan Vassilev, Shaul Reuveny and Steve Kah Weng Oh (2022) An allied reprogramming, selection, expansion and differentiation platform for creating hiPSC on microcarriers. Cell Proliferation 55:e13256
- Alan Tin-Lun Lam, Alison P. Lee, Premkumar Jayaraman, Kah Yong Tan, Deepak Raghothaman, Hsueh Lee Lim, He Cheng, Lihan Zhou, Andy Hee-Meng Tan, Shaul Reuveny and Steve Oh (2021) Multiomics analyses of cytokines, gene, miRNA, and pathway regulatory networks in human mesenchymal stem cells expanded in stirred microcarrier-spinner cultures. Stem Cell Research 53:102272
- Jaichandran Sivalingam, Yu SuE, Zhong Ri Lim, Alan T.L. Lam, Alison P. Lee, Hsueh Lee Lim, Hong Yu Chen, Hong Kee Tan, Tushar Warrier, Jing Wen Hang, Nazmi B. Nazir, Andy H.M. Tan, Laurent Renia, Yuin Han Loh, Shaul Reuveny, Benoit Malleret and Steve K.W. Oh (2020) A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells. Stem Cell Reports 16(1): 182-197
- Alan Tin-Lun Lam, Allen Kuan-Liang Chen, Sherwin Qi-Peng Ting, Shaul Reuveny and Steve Kah-Weng Oh (2016) Integrated processes for expansion and differentiation of human pluripotent stem cells in suspended microcarriers cultures. Biochemical and Biophysical Research Communications 473(3): 764-8
Featured Publications
- Microcarriers for stem cell culture (2014)
- Culture of pluripotent and multipotent cells on microcarriers (2014)
The Team
Dr Alan Lam
alan_lam@bti.a-star.edu.sg
Senior Scientist II
PhD in Biochemistry (2003), The Chinese University of Hong Kong, Hong Kong
Research Focus / Interest
- Scalable production of human embryonic stem cells, human induced pluripotent stem cells and human mesenchymal stem cells and its differentiated cells
- Medium development for large-scale expansion of mesenchymal stem cells in bioreactors
Dr Leonard Leong
leonard_leong@bti.a-star.edu.sg
Scientist
PhD in Immunology (2021), NUS Graduate School (Integrative Sciences and Engineering Programme), National University of Singapore, Singapore
Research Focus / Interest
- Development of novel chimeric antigen receptors for adoptive immunotherapy
- Scalable production and differentiation of induced pluripotent stem cells into immune cell subsets
- Development of allogeneic cell therapeutics for anti-cancer applications
