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Our Capabilities
SYNTHETIC & PROTEIN ENGINEERING
Strain and toolbox development for protein production
MICROBIAL FERMENTATION FOR VARIOUS HOSTS
Competency in working with various microbial hosts such as E. coli, LAB & Yeast
MICROBIAL PRODUCTION OF RECOMBINANT PROTEINS
Production of recombinant growth factors, vaccines & other proteins
EVALUATION OF COMMERCIAL SYSTEMS & MEDIA
Bioreactor cultivation in batch, fed-batch and perfusion modes up to 50L
VECTOR DEVELOPMENT
Stringent control of vector expression with optimal secretion & productivity
SUPPORTING PARTNERS IN PROOF-OF-CONCEPT STUDIES
Collaborate in the production of molecules for proof-of-concept studies and research projects.
With our main focus in microbial biomanufacturing of protein biologics, MCB has developed a wide range of end-to-end capabilities and technologies. This ranges from microbial host engineering, in-silico models, fermentation options and bioprocesses down to purification and product characterisation. We also seek to accelerate personalized microbiome therapeutics as well as to expand the knowledge of microbial biomanufacturing capability.
Our Technologies
Production of recombinant growth factors for cultivated meat
Our team is developing an alternate source of scalable serum-substitute for animal-derived serum, such as FBS (Fetal Bovine Serum). This is part of our growing efforts to make cultured meat commercially viable and ethical for consumption.
Propeptide toolbox for improved secretion of recombinant proteins
Lactic acid bacteria (LAB) are a promising family of food-grade organisms for recombinant protein manufacturing. Our team have in silico-mined novel propeptides, which enhanced secretion yield and enzyme activity, making this toolbox useful for secretion optimisation of other recombinant proteins found in LAB.
Cell anchoring domain for heterologous protein display on LAB
Our team taps on the advantage of LAB as a useful adjuvant and delivery vector for vaccine. We have engineered a novel anchoring domain (CAD4a) that enables therapeutic proteins to be coated on the surface of LAB, employing them as drug delivery vehicles.
Our Track Record
Featured Publications
- Bryan Nicholas Chua, Wei Mei Guo, Han Teng Wong, Dave Siak-Wei Ow, Pooi Leng Ho, Winston Koh, Ann Koay and Fong Tian Wong (2023) A sweeter future: Using protein language models for exploring sweeter brazzein homologs. Food Chemistry 426:136580
- Lokanand Koduru, Meiyappan Lakshmanan, Yi Qing Lee, Pooi-Leng Ho, Pei-Yu Lim, Wei Xuan Ler, Say Kong Ng, Dongseok Kim, Doo-Sang Park, Mazlina Banu, Dave Siak Wei Ow and Dong-Yup Lee (2022) Systematic evaluation of genome-wide metabolic landscapes in lactic acid bacteria reveals diet-induced and strain-specific probiotic idiosyncrasies. Cell Reports 41: 111735
- Dave Siak Wei Ow, Min-Kyu Oh, Chung-Jen Chiang and Yun-Peng Chao (2021) Technological Advances Improving Recombinant Protein Production in Bacteria. Frontiers in Microbiology 12: 729472
- Pei Kun Richie Tay, Pei-Yu Lim and Dave Siak Wei Ow (2021) A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria. Frontiers in Bioengineering and Biotechnology 8: 614498
- Pooi-Leng Ho, Han Kee Ong, Jeanette Teo, Dave Siak Wei Ow and Sheng-Hao Chao (2019) HEXIM1 peptide exhibits antimicrobial activity against antibiotic resistant bacteria through guidance of cell penetrating peptide. Frontiers in Microbiology 10: 203
The Team
Dr Dave Ow
dave_ow@bti.a-star.edu.sg
Principal Scientist I
PhD in Integrative Sciences and Engineering (2007), National University of Singapore, Singapore
Research Focus / Interest
- Analysis of cellular phospho-signaling and metabolic networks using a systems-level proteomics and transcriptomics approach
- Development of acoustically driven microfluidic devices for biological applications
- Metabolic engineering and production of recombinant proteins and DNA plasmids from microbial cells
Dr Prashant Mainali
prashant_mainali@bti.a-star.edu.sg
Scientist
PhD in Biomedical Engineering (2023), National University of Singapore, Singapore
Research Focus / Interest
- Process optimization for the production of metabolites, and proteins from E. coli and Lactic Acid bacteria
- Development of structured bioprocess model for E. coli and Lactic Acid bacteria
- Study of metabolic pathway reorganization after induction in an engineered microorganism
