Huck Hui NG

Huck Hui NG

Senior Group Leader,
Laboratory of Precision Disease Therapeutics




Our ability to reprogram somatic cells to a pluripotent state (from which differentiation into various cell lineages can be induced), as well as the capability to isolate and propagate adult stem cells from human tissues, are revolutionizing both our understanding of basic stem cell biology as well as approaches to disease modeling. While the technology is still at its infancy, we have begun to witness the potential of stem cells in multiple applications ranging from regenerative therapy to disease modeling in a dish. To harness the full capacity of these stem cells, we aim to address two fundamental questions 1) how pluripotent and adult stem cells maintain their unique cell states, and 2) how we can leverage their propensities for controlled differentiation for the creation of next-generation cell and tissue models. We utilize advanced genetic approaches to identify and interrogate regulatory machineries governing the stem cell state. This is coupled with the development of novel cell culture approaches to generate 3D cellular models that demonstrate higher-order structure and a greater repertoire of relevant cell types to closely mimic the in vivo cell niche. We are particularly interested in applying these techniques to understand etiologies of neurodegenerative and metabolic diseases. Our ultimate goal is to harness the power of stem cells to derive more physiological disease models that can drive novel therapeutic discoveries and eventually actualize the goal of personalized medicine.

**We welcome talented postdoctoral fellows, computational biologists and students to join us. Interested candidates may forward their CVs to Prof Ng: cc his PA:**

Selected Publications

  • Yang L, Ng HH "Lab-grown mini-brains upgraded." Nat Cell Biol 2017 Aug 31 ; 19(9) : 1010-1012 Abstract
  • Jo J, Xiao Y, Sun AX, Cukuroglu E, Tran HD, Göke J, Tan ZY, Saw TY, Tan CP, Lokman H, Lee Y, Kim D, Ko HS, Kim SO, Park JH, Cho NJ, Hyde TM, Kleinman JE, Shin JH, Weinberger DR, Tan EK, Je HS, Ng HH "Midbrain-like Organoids from Human Pluripotent Stem Cells Contain Functional Dopaminergic and Neuromelanin-Producing Neurons." Cell Stem Cell 2016 Aug 04 ; 19(2) : 248-257 Abstract
  • Gonzales KA, Liang H, Lim YS, Chan YS, Yeo JC, Tan CP, Gao B, Le B, Tan ZY, Low KY, Liou YC, Bard F, Ng HH "Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways." Cell 2015 Jul 30 ; 162(3) : 564-79 Abstract
  • Yeo JC, Jiang J, Tan ZY, Yim GR, Ng JH, Göke J, Kraus P, Liang H, Gonzales KA, Chong HC, Tan CP, Lim YS, Tan NS, Lufkin T, Ng HH "Klf2 is an essential factor that sustains ground state pluripotency." Cell Stem Cell 2014 Jun 5 ; 14(6) : 864-72 Abstract
  • Chan YS, Göke J, Ng JH, Lu X, Gonzales KA, Tan CP, Tng WQ, Hong ZZ, Lim YS, Ng HH "Induction of a human pluripotent state with distinct regulatory circuitry that resembles preimplantation epiblast." Cell Stem Cell 2013 Dec 5 ; 13(6) : 663-75 Abstract
  • Chia NY, Chan YS, Feng B, Lu X, Orlov YL, Moreau D, Kumar P, Yang L, Jiang J, Lau MS, Huss M, Soh BS, Kraus P, Li P, Lufkin T, Lim B, Clarke ND, Bard F, Ng HH "A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity." Nature 2010 Nov 11 ; 468(7321) : 316-20 Epub 2010 Oct 17 Abstract
  • Feng Bo, Jiang Jianming, Kraus Petra, Ng Jia-Hui, Heng Jian-Chien Dominic, Chan Yun-Shen, Yaw Lai-Ping, Zhang Weiwei, Loh Yuin-Han, Han Jianyong, Vega Vinsensius B, Cacheux-Rataboul Valere, Lim Bing, Lufkin Thomas, Ng Huck-Hui "Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb." Nat. Cell Biol. 2009 Feb ; 11(2) : 197-203 Abstract
  • Chen Xi, Xu Han, Yuan Ping, Fang Fang, Huss Mikael, Vega Vinsensius B, Wong Eleanor, Orlov Yuriy L, Zhang Weiwei, Jiang Jianming, Loh Yuin-Han, Yeo Hock Chuan, Yeo Zhen Xuan, Narang Vipin, Govindarajan Kunde Ramamoorthy, Leong Bernard, Shahab Atif, Ruan Yijun, Bourque Guillaume, Sung Wing-Kin, Clarke Neil D, Wei Chia-Lin, Ng Huck-Hui "Integration of external signaling pathways with the core transcriptional network in embryonic stem cells." Cell 2008 Jun 13 ; 133(6) : 1106-17 Abstract
  • Loh Yuin-Han, Zhang Weiwei, Chen Xi, George Joshy, Ng Huck-Hui "Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells." Genes Dev. 2007 Oct 15 ; 21(20) : 2545-57 Abstract
  • Loh Yuin-Han, Wu Qiang, Chew Joon-Lin, Vega Vinsensius B, Zhang Weiwei, Chen Xi, Bourque Guillaume, George Joshy, Leong Bernard, Liu Jun, Wong Kee-Yew, Sung Ken W, Lee Charlie W H, Zhao Xiao-Dong, Chiu Kuo-Ping, Lipovich Leonard, Kuznetsov Vladimir A, Robson Paul, Stanton Lawrence W, Wei Chia-Lin, Ruan Yijun, Lim Bing, Ng Huck-Hui "The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells." Nat. Genet. 2006 Apr ; 38(4) : 431-40 Abstract