Chew Wei Leong

RESEARCH

We build technologies to rewrite the genome | Our research broadly covers synthetic biology and technology development, with focus on genome-editing, immunology, and gene therapy. To invent genome engineering technologies, we capitalize on nucleic acid manipulation, protein engineering, directed evolution, and high-throughput methodologies. Our overarching motivation is to establish safe and efficacious genome therapeutics for routine human use.

Lab Website
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* We are recruiting; email and come chat!

Selected Publications

• A.M. Moreno, N. Palmer, F. Aleman, G. Chen, A. Pla, W. L. Chew, M. Law, P. Mali. Exploring protein orthogonality in immune space: a case study with AAV and Cas9 orthologs. bioRxiv 245985. Abstract
• G. M. Church and W. L. Chew. AAV split Cas9 genome editing and transcriptional regulation. PCT/US2017/032362, WO/2017/197238. Abstract
• W. L. Chew. Immunity to CRISPR Cas9 and Cas12a therapeutics. Wiley Interdisciplinary Reviews Systems Biology and Medicine 10.1002/wsbm.1408. (2017) Abstract
• L. Yang, A. Briggs, W. L. Chew, P. Mali, M. Guell, J. Aach, D. Goodman, D. Cox, Y. Kan, E. Lesha, V. Soundararajan, F. Zhang, G. M. Church. Engineering and optimising deaminase fusions for genome editing. Nature communications 7, 13330 (2016); published online Epub 2016 Nov 02 (10.1038/ncomms13330). bioRxiv 066597. Abstract
• L. Zangi, M. S. Oliveira, L. Y. Ye, Q. Ma, N. Sultana, Y. Hadas, E. Chepurko, D. Später, B. Zhou, W. L. Chew, W. Ebina, M. Abrial, Q. D. Wang, W. T. Pu, and K. R. Chien. An IGF1R-Dependent Pathway Drives Epicardial Adipose Tissue Formation After Myocardial Injury. Circulation 135, 59-72 (2017); published online Epub 2016 Nov 01 (10.1161/CIRCULATIONAHA.116.022064). Abstract
• W. L. Chew, M. Tabebordbar, J. K. Cheng, P. Mali, E. Y. Wu, A. H. Ng, K. Zhu, A. J. Wagers, G. M. Church, A multifunctional AAV-CRISPR-Cas9 and its host response. Nature methods 13, 868-874 (2016); published online Epub 2016 Sep 05 (10.1038/nmeth.3993). Abstract
• G. M. Church and W. L. Chew. Split Cas9 proteins. PCT/US2016/012570, WO/2016/112242. Abstract
• A. J. Wagers, M. Tabebordbar, W. L. Chew, and G. M. Church. RNA-guided systems for in vivo gene editing. PCT/US2015/063181, WO/2016/089866. Abstract
• W.L. Chew. Postnatal Genome Editing With CRISPR. (2016). Abstract
• M. Tabebordbar, K. Zhu, J. K. Cheng, W. L. Chew, J. J. Widrick, W. X. Yan, C. Maesner, E. Y. Wu, R. Xiao, F. A. Ran, L. Cong, F. Zhang, L. H. Vandenberghe, G. M. Church, A. J. Wagers, In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Science 351, 407-411 (2016); published online Epub 2015 Dec 31 (10.1126/science.aad5177). Abstract