SEOW Yiqi

RESEARCH

Nucleic acid diagnostics has proven its worth during the Covid-19 pandemic and there are a growing number of indications that can be diagnosed with nucleic acid analytes. However, while new diagnostic biomarkers such as methylated DNA and single cell transcriptome are being shown to improve diagnostic capabilities for diseases, current methods can be cost-prohibitive for routine diagnostics. Seow Yiqi is looking at the workflow for nucleic acid diagnostics and asking if there are technologies that can make nucleic acid diagnostics more affordable and deployable, thus allowing patients to access these new biomarkers more affordably. He has developed and licenced out a nucleic acid extraction kit; an unbiased metagenomic sequencing workflow for affordable rapid identification of pathogens; and developed lyophilization nucleic acid detection reagents. He is also keen to support companies by assisting in developing in-house nucleic acid extraction, lyophilization, sequencing kit development and nucleic acid processing capabilities to enhance their product portfolio.

Selected Publications

• Koh LCW, Seow Y, Kong KW, Lau MLL, Kumar SK, Yan G, Lee CK, Yan B, Tambyah PA, Hoon S. "Sub genomic analysis of SARS-CoV-2 using short read amplicon-based sequencing." Front Genet. 2023 Feb 24;14:1086865. doi: 10.3389/fgene.2023.1086865. eCollection 2023. PMID: 36911398. Free PMC article.
• Lin C, Han G, Jia L, Zhao Y, Song J, Ran N, Yokota T, Seow Y, Yin H. "Cardio-respiratory and phenotypic rescue of dystrophin/utrophin-deficient mice by combination therapy."EMBO Rep. 2022 Jun 7;23(6):e53955. doi: 10.15252/embr.202153955. Epub 2022 Apr 8. PMID: 35393769
• Beh CW, Yew DS, Chai RJ, Chin SY, Seow Y, Hoon SS. "A fluid-supported 3D hydrogel bioprinting method."Biomaterials. 2021 Sep;276:121034. doi: 10.1016/j.biomaterials.2021.121034. Epub 2021 Jul 21. PMID: 34332372
• Gupta D, Wiklander OPB, Görgens A, Conceição M, Corso G, Liang X, Seow Y, Balusu S, Feldin U, Bostancioglu B, Jawad R, Mamand DR, Lee YXF, Hean J, Mäger I, Roberts TC, Gustafsson M, Mohammad DK, Sork H, Backlund A, Lundin P, de Fougerolles A, Smith CIE, Wood MJA, Vandenbroucke RE, Nordin JZ, El-Andaloussi S. "Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles."Nat Biomed Eng. 2021 Sep;5(9):1084-1098. doi: 10.1038/s41551-021-00792-z. Epub 2021 Oct 6. PMID: 34616047
• Fan C, El Andaloussi S, Lehto T, Kong KW, Seow Y. "Smad‑binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts."Mol Med Rep. 2020 Dec;22(6):4589-4600. doi: 10.3892/mmr.2020.11549. Epub 2020 Sep 29. PMID: 33173952. Free PMC article.
• Gao X, Ran N, Dong X, Zuo B, Yang R, Zhou Q, Moulton HM, Seow Y, Yin H. "Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy."Sci Transl Med. 2018 Jun 6;10(444):eaat0195. doi: 10.1126/scitranslmed.aat0195. PMID: 29875202
• Han G, Gu B, Cao L, Gao X, Wang Q, Seow Y, Zhang N, Wood MJ, Yin H. "Hexose enhances oligonucleotide delivery and exon skipping in dystrophin-deficient mdx mice."Nat Commun. 2016 Mar 11;7:10981. doi: 10.1038/ncomms10981. PMID: 26964641. Free PMC article.
• Kock KH, Kong KW, Hoon S, Seow Y. "Functional VEGFA knockdown with artificial 3'-tailed mirtrons defined by 5' splice site and branch point.Nucleic Acids Res. 2015 Jul 27;43(13):6568-78. doi: 10.1093/nar/gkv617. Epub 2015 Jun 18. PMID: 26089392. Free PMC article.
• Guerette PA, Hoon S, Seow Y, Raida M, Masic A, Wong FT, Ho VH, Kong KW, Demirel MC, Pena-Francesch A, Amini S, Tay GZ, Ding D, Miserez A. "Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science."Nat Biotechnol. 2013 Oct;31(10):908-15. doi: 10.1038/nbt.2671. Epub 2013 Sep 8. PMID: 24013196
• Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood MJ. "Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes."Nat Biotechnol. 2011 Apr;29(4):341-5. doi: 10.1038/nbt.1807. Epub 2011 Mar 20. PMID: 21423189